Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Il1rap

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Il1rap N/A
Sequence Length (AA) Molecular Weight (Da)
570 65741
Protein Name
Interleukin-1 receptor accessory protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLLWYLMSL SFYGILQSHA SERCDDWGLD TMRQIQVFED EPARIKCPLF
60 70 80 90 100
EHFLKYNYST AHSSGLTLIW YWTRQDRDLE EPINFRLPEN RISKEKDVLW
110 120 130 140 150
FRPTLLNDTG NYTCMLRNTT YCSKVAFPLE VVQKDSCFNS AMRFPVHKMY
160 170 180 190 200
IEHGIHKITC PNVDGYFPSS VKPSVTWYKG CTEIVDFHNV LPEGMNLSFF
210 220 230 240 250
IPLVSNNGNY TCVVTYPENG RLFHLTRTVT VKVVGSPKDA LPPQIYSPND
260 270 280 290 300
RVVYEKEPGE ELVIPCKVYF SFIMDSHNEV WWTIDGKKPD DVTVDITINE
310 320 330 340 350
SVSYSSTEDE TRTQILSIKK VTPEDLRRNY VCHARNTKGE AEQAAKVKQK
360 370 380 390 400
VIPPRYTVEL ACGFGATVFL VVVLIVVYHV YWLEMVLFYR AHFGTDETIL
410 420 430 440 450
DGKEYDIYVS YARNVEEEEF VLLTLRGVLE NEFGYKLCIF DRDSLPGGIV
460 470 480 490 500
TDETLSFIQK SRRLLVVLSP NYVLQGTQAL LELKAGLENM ASRGNINVIL
510 520 530 540 550
VQYKAVKDMK VKELKRAKTV LTVIKWKGEK SKYPQGRFWK QLQVAMPVKK
560 570
SPRWSSNDKQ GLSYSSLKNV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-2221 Collapse assay details

Data source: Panorama

Official Gene Symbol
IL1RAP
Peptide Modified Sequence
N[+1.0]TTYC[+57.0]SK
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
118, 122
Peptide - Site of Modification
1, 5
Peptide Start
118
Peptide End
124
CPTAC ID
CPTAC-2221
Peptide Molecular Mass
873.3538
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.5 10 9.5 17.2 17.4 14 27.5 20.1 16.9 15 15 15
y4 (1+) 11.2 11.6 9.3 13.6 6.2 9.2 17.6 13.2 13.1 15 15 15
y5 (1+) 13.1 8.2 6.7 12.4 7.8 7.3 18 11.3 9.9 15 15 15
sum 8.2 5.5 4 8.4 5.9 4.4 11.7 8.1 5.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3320 Collapse assay details

Data source: Panorama

Official Gene Symbol
Il1rap
Peptide Sequence
VAFPLEVVQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
124
Peptide End
134
CPTAC ID
non-CPTAC-3320
Peptide Molecular Mass
1,128.6543
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 5.6 5.8 3.8 15 7.5 8.4 16 9.5 9.2 15 15 15
y8 (1+) 5.3 3.9 4.4 15 7.7 7.2 15.9 8.6 8.4 15 15 15
y7 (1+) 4.2 4.8 5.2 13.9 5.5 5.7 14.5 7.3 7.7 15 15 15
y7 (2+) 5.8 3.4 4.8 12.9 5.8 6.7 14.1 6.7 8.2 15 15 15
b3 (1+) 6.2 4.7 5.6 15 5.8 6.4 16.2 7.5 8.5 15 15 15
sum 2.9 2.2 2.7 13.7 5.1 6.4 14 5.6 6.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments