IL1RAP, CPTAC-2221 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IL1RAP	C3orf13, IL1R3

Sequence Length (AA)	Molecular Weight (Da)
570	65418

Protein Name
Interleukin-1 receptor accessory protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTLLWCVVSL	YFYGILQSDA	SERCDDWGLD	TMRQIQVFED	EPARIKCPLF
60	70	80	90	100
EHFLKFNYST	AHSAGLTLIW	YWTRQDRDLE	EPINFRLPEN	RISKEKDVLW
110	120	130	140	150
FRPTLLNDTG	NYTCMLRNTT	YCSKVAFPLE	VVQKDSCFNS	PMKLPVHKLY
160	170	180	190	200
IEYGIQRITC	PNVDGYFPSS	VKPTITWYMG	CYKIQNFNNV	IPEGMNLSFL
210	220	230	240	250
IALISNNGNY	TCVVTYPENG	RTFHLTRTLT	VKVVGSPKNA	VPPVIHSPND
260	270	280	290	300
HVVYEKEPGE	ELLIPCTVYF	SFLMDSRNEV	WWTIDGKKPD	DITIDVTINE
310	320	330	340	350
SISHSRTEDE	TRTQILSIKK	VTSEDLKRSY	VCHARSAKGE	VAKAAKVKQK
360	370	380	390	400
VPAPRYTVEL	ACGFGATVLL	VVILIVVYHV	YWLEMVLFYR	AHFGTDETIL
410	420	430	440	450
DGKEYDIYVS	YARNAEEEEF	VLLTLRGVLE	NEFGYKLCIF	DRDSLPGGIV
460	470	480	490	500
TDETLSFIQK	SRRLLVVLSP	NYVLQGTQAL	LELKAGLENM	ASRGNINVIL
510	520	530	540	550
VQYKAVKETK	VKELKRAKTV	LTVIKWKGEK	SKYPQGRFWK	QLQVAMPVKK
560	570
SPRRSSSDEQ	GLSYSSLKNV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2221 Collapse assay details

Data source: Panorama

Official Gene Symbol: IL1RAP
Peptide Modified Sequence: N[+1.0]TTYC[+57.0]SK
Modification Type: Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification: 118, 122
Peptide - Site of Modification: 1, 5
Peptide Start: 118
Peptide End: 124

CPTAC ID: CPTAC-2221
Peptide Molecular Mass: 873.3538
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	21.5	10	9.5	17.2	17.4	14	27.5	20.1	16.9	15	15	15
y4 (1+)	11.2	11.6	9.3	13.6	6.2	9.2	17.6	13.2	13.1	15	15	15
y5 (1+)	13.1	8.2	6.7	12.4	7.8	7.3	18	11.3	9.9	15	15	15
sum	8.2	5.5	4	8.4	5.9	4.4	11.7	8.1	5.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3320 Collapse assay details

Data source: Panorama

Official Gene Symbol: Il1rap
Peptide Sequence: VAFPLEVVQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 124
Peptide End: 134

CPTAC ID: non-CPTAC-3320
Peptide Molecular Mass: 1,128.6543
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.