GALNT5, CPTAC-2216 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GALNT5	N/A

Sequence Length (AA)	Molecular Weight (Da)
940	106266

Protein Name
Polypeptide N-acetylgalactosaminyltransferase 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNRIRKFFRG	SGRVLAFIFV	ASVIWLLFDM	AALRLSFSEI	NTRVIKEDIV
60	70	80	90	100
RRERIGFRVQ	PDQGKIFYSS	IKEMKPPLRG	HGKGAWGKEN	VRKTEESVLK
110	120	130	140	150
VEVDLDQTQR	ERKMQNALGR	GKVVPLWHPA	HLQTLPVTPN	KQKTDGRGTK
160	170	180	190	200
PEASSHQGTP	KQTTAQGAPK	TSFIAAKGTQ	VVKISVHMGR	VSLKQEPRKS
210	220	230	240	250
HSPSSDTSKL	AAERDLNVTI	SLSTDRPKQR	SQAVANERAH	PASTAVPKSG
260	270	280	290	300
EAMALNKTKT	QSKEVNANKH	KANTSLPFPK	FTVNSNRLRK	QSINETPLGS
310	320	330	340	350
LSKDDGARGA	HGKKLNFSES	HLVIITKEEE	QKADPKEVSN	SKTKTIFPKV
360	370	380	390	400
LGKSQSKHIS	RNRSEMSSSS	LAPHRVPLSQ	TNHALTGGLE	PAKINITAKA
410	420	430	440	450
PSTEYNQSHI	KALLPEDSGT	HQVLRIDVTL	SPRDPKAPGQ	FGRPVVVPHG
460	470	480	490	500
KEKEAERRWK	EGNFNVYLSD	LIPVDRAIED	TRPAGCAEQL	VHNNLPTTSV
510	520	530	540	550
IMCFVDEVWS	TLLRSVHSVI	NRSPPHLIKE	ILLVDDFSTK	DYLKDNLDKY
560	570	580	590	600
MSQFPKVRIL	RLKERHGLIR	ARLAGAQNAT	GDVLTFLDSH	VECNVGWLEP
610	620	630	640	650
LLERVYLSRK	KVACPVIEVI	NDKDMSYMTV	DNFQRGIFVW	PMNFGWRTIP
660	670	680	690	700
PDVIAKNRIK	ETDTIRCPVM	AGGLFSIDKS	YFFELGTYDP	GLDVWGGENM
710	720	730	740	750
ELSFKVWMCG	GEIEIIPCSR	VGHIFRNDNP	YSFPKDRMKT	VERNLVRVAE
760	770	780	790	800
VWLDEYKELF	YGHGDHLIDQ	GLDVGNLTQQ	RELRKKLKCK	SFKWYLENVF
810	820	830	840	850
PDLRAPIVRA	SGVLINVALG	KCISIENTTV	ILEDCDGSKE	LQQFNYTWLR
860	870	880	890	900
LIKCGEWCIA	PIPDKGAVRL	HPCDNRNKGL	KWLHKSTSVF	HPELVNHIVF
910	920	930	940
ENNQQLLCLE	GNFSQKILKV	AACDPVKPYQ	KWKFEKYYEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2216 Collapse assay details

Data source: Panorama

Official Gene Symbol: GALNT5
Peptide Modified Sequence: AN[+1.0]TSLPFPK
Modification Type: Deamidated (N)
Protein - Site of Modification: 273
Peptide - Site of Modification: 2
Peptide Start: 272
Peptide End: 280

CPTAC ID: CPTAC-2216
Peptide Molecular Mass: 974.5073
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	11.9	6.3	7.9	11.4	5.7	8.3	16.5	8.5	11.5	15	15	15
y6 (1+)	16.9	9.3	9.5	16.2	10.1	10.7	23.4	13.7	14.3	15	15	15
y7 (1+)	13.6	6.5	8.5	10.8	9.2	10.2	17.4	11.3	13.3	15	15	15
sum	8.6	5.3	4.9	8.6	4.4	5.8	12.2	6.9	7.6	15	15	15

GALNT5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2216 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

GALNT5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2216 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments