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Overview Data source: UniProt

Official Gene Symbol Other Aliases
GALNT5 N/A
Sequence Length (AA) Molecular Weight (Da)
940 106266
Protein Name
Polypeptide N-acetylgalactosaminyltransferase 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNRIRKFFRG SGRVLAFIFV ASVIWLLFDM AALRLSFSEI NTRVIKEDIV
60 70 80 90 100
RRERIGFRVQ PDQGKIFYSS IKEMKPPLRG HGKGAWGKEN VRKTEESVLK
110 120 130 140 150
VEVDLDQTQR ERKMQNALGR GKVVPLWHPA HLQTLPVTPN KQKTDGRGTK
160 170 180 190 200
PEASSHQGTP KQTTAQGAPK TSFIAAKGTQ VVKISVHMGR VSLKQEPRKS
210 220 230 240 250
HSPSSDTSKL AAERDLNVTI SLSTDRPKQR SQAVANERAH PASTAVPKSG
260 270 280 290 300
EAMALNKTKT QSKEVNANKH KANTSLPFPK FTVNSNRLRK QSINETPLGS
310 320 330 340 350
LSKDDGARGA HGKKLNFSES HLVIITKEEE QKADPKEVSN SKTKTIFPKV
360 370 380 390 400
LGKSQSKHIS RNRSEMSSSS LAPHRVPLSQ TNHALTGGLE PAKINITAKA
410 420 430 440 450
PSTEYNQSHI KALLPEDSGT HQVLRIDVTL SPRDPKAPGQ FGRPVVVPHG
460 470 480 490 500
KEKEAERRWK EGNFNVYLSD LIPVDRAIED TRPAGCAEQL VHNNLPTTSV
510 520 530 540 550
IMCFVDEVWS TLLRSVHSVI NRSPPHLIKE ILLVDDFSTK DYLKDNLDKY
560 570 580 590 600
MSQFPKVRIL RLKERHGLIR ARLAGAQNAT GDVLTFLDSH VECNVGWLEP
610 620 630 640 650
LLERVYLSRK KVACPVIEVI NDKDMSYMTV DNFQRGIFVW PMNFGWRTIP
660 670 680 690 700
PDVIAKNRIK ETDTIRCPVM AGGLFSIDKS YFFELGTYDP GLDVWGGENM
710 720 730 740 750
ELSFKVWMCG GEIEIIPCSR VGHIFRNDNP YSFPKDRMKT VERNLVRVAE
760 770 780 790 800
VWLDEYKELF YGHGDHLIDQ GLDVGNLTQQ RELRKKLKCK SFKWYLENVF
810 820 830 840 850
PDLRAPIVRA SGVLINVALG KCISIENTTV ILEDCDGSKE LQQFNYTWLR
860 870 880 890 900
LIKCGEWCIA PIPDKGAVRL HPCDNRNKGL KWLHKSTSVF HPELVNHIVF
910 920 930 940
ENNQQLLCLE GNFSQKILKV AACDPVKPYQ KWKFEKYYEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2216 Collapse assay details

Data source: Panorama

Official Gene Symbol
GALNT5
Peptide Modified Sequence
AN[+1.0]TSLPFPK
Modification Type
Deamidated (N)
Protein - Site of Modification
273
Peptide - Site of Modification
2
Peptide Start
272
Peptide End
280
CPTAC ID
CPTAC-2216
Peptide Molecular Mass
974.5073
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.9 6.3 7.9 11.4 5.7 8.3 16.5 8.5 11.5 15 15 15
y6 (1+) 16.9 9.3 9.5 16.2 10.1 10.7 23.4 13.7 14.3 15 15 15
y7 (1+) 13.6 6.5 8.5 10.8 9.2 10.2 17.4 11.3 13.3 15 15 15
sum 8.6 5.3 4.9 8.6 4.4 5.8 12.2 6.9 7.6 15 15 15

Additional Resources and Comments