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Overview Data source: UniProt

Official Gene Symbol Other Aliases
NAGA N/A
Sequence Length (AA) Molecular Weight (Da)
411 46565
Protein Name
Alpha-N-acetylgalactosaminidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLLKTVLLLG HVAQVLMLDN GLLQTPPMGW LAWERFRCNI NCDEDPKNCI
60 70 80 90 100
SEQLFMEMAD RMAQDGWRDM GYTYLNIDDC WIGGRDASGR LMPDPKRFPH
110 120 130 140 150
GIPFLADYVH SLGLKLGIYA DMGNFTCMGY PGTTLDKVVQ DAQTFAEWKV
160 170 180 190 200
DMLKLDGCFS TPEERAQGYP KMAAALNATG RPIAFSCSWP AYEGGLPPRV
210 220 230 240 250
NYSLLADICN LWRNYDDIQD SWWSVLSILN WFVEHQDILQ PVAGPGHWND
260 270 280 290 300
PDMLLIGNFG LSLEQSRAQM ALWTVLAAPL LMSTDLRTIS AQNMDILQNP
310 320 330 340 350
LMIKINQDPL GIQGRRIHKE KSLIEVYMRP LSNKASALVF FSCRTDMPYR
360 370 380 390 400
YHSSLGQLNF TGSVIYEAQD VYSGDIISGL RDETNFTVII NPSGVVMWYL
410 411
YPIKNLEMSQ Q

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2228 Collapse assay details

Data source: Panorama

Official Gene Symbol
NAGA
Peptide Modified Sequence
LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK
Modification Type
Oxidation (M), Deamidated (N), Carbamidomethyl (C), Oxidation (M)
Protein - Site of Modification
122, 124, 127, 128
Peptide - Site of Modification
7, 9, 12, 13
Peptide Start
116
Peptide End
137
CPTAC ID
CPTAC-2228
Peptide Molecular Mass
2,457.0538
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 50.6 17.2 16 40.8 35 23.9 65 39 28.8 15 15 15
y11 (2+) 26 18.9 12.6 36.2 19.3 11.8 44.6 27 17.3 15 15 15
y7 (1+) 12.1 6.4 9.1 13 7.3 7.6 17.8 9.7 11.9 15 15 15
sum 8.6 7.9 7.6 10.9 8.2 7.2 13.9 11.4 10.5 15 15 15

Additional Resources and Comments