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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FUCA1 N/A
Sequence Length (AA) Molecular Weight (Da)
466 53689
Protein Name
Tissue alpha-L-fucosidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRAPGMRSRP AGPALLLLLL FLGAAESVRR AQPPRRYTPD WPSLDSRPLP
60 70 80 90 100
AWFDEAKFGV FIHWGVFSVP AWGSEWFWWH WQGEGRPQYQ RFMRDNYPPG
110 120 130 140 150
FSYADFGPQF TARFFHPEEW ADLFQAAGAK YVVLTTKHHE GFTNWPSPVS
160 170 180 190 200
WNWNSKDVGP HRDLVGELGT ALRKRNIRYG LYHSLLEWFH PLYLLDKKNG
210 220 230 240 250
FKTQHFVSAK TMPELYDLVN SYKPDLIWSD GEWECPDTYW NSTNFLSWLY
260 270 280 290 300
NDSPVKDEVV VNDRWGQNCS CHHGGYYNCE DKFKPQSLPD HKWEMCTSID
310 320 330 340 350
KFSWGYRRDM ALSDVTEESE IISELVQTVS LGGNYLLNIG PTKDGLIVPI
360 370 380 390 400
FQERLLAVGK WLSINGEAIY ASKPWRVQWE KNTTSVWYTS KGSAVYAIFL
410 420 430 440 450
HWPENGVLNL ESPITTSTTK ITMLGIQGDL KWSTDPDKGL FISLPQLPPS
460 466
AVPAEFAWTI KLTGVK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2215 Collapse assay details

Data source: Panorama

Official Gene Symbol
FUCA1
Peptide Modified Sequence
N[+1.0]TTSVWYTSK
Modification Type
Deamidated (N)
Protein - Site of Modification
382
Peptide - Site of Modification
1
Peptide Start
382
Peptide End
391
CPTAC ID
CPTAC-2215
Peptide Molecular Mass
1,186.5506
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 17.3 11.2 9.1 16.1 12.2 5.9 23.6 16.6 10.8 15 15 15
y7 (1+) 16 6.7 12.6 17.6 11.6 15.6 23.8 13.4 20.1 15 15 15
y5 (1+) 9.7 6.2 6.7 11.6 7.7 8.3 15.1 9.9 10.7 15 15 15
sum 7.5 4.9 5.5 7 4.8 6.3 10.3 6.9 8.4 15 15 15

Additional Resources and Comments