FUCA1, CPTAC-2215 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FUCA1	N/A

Sequence Length (AA)	Molecular Weight (Da)
466	53689

Protein Name
Tissue alpha-L-fucosidase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRAPGMRSRP	AGPALLLLLL	FLGAAESVRR	AQPPRRYTPD	WPSLDSRPLP
60	70	80	90	100
AWFDEAKFGV	FIHWGVFSVP	AWGSEWFWWH	WQGEGRPQYQ	RFMRDNYPPG
110	120	130	140	150
FSYADFGPQF	TARFFHPEEW	ADLFQAAGAK	YVVLTTKHHE	GFTNWPSPVS
160	170	180	190	200
WNWNSKDVGP	HRDLVGELGT	ALRKRNIRYG	LYHSLLEWFH	PLYLLDKKNG
210	220	230	240	250
FKTQHFVSAK	TMPELYDLVN	SYKPDLIWSD	GEWECPDTYW	NSTNFLSWLY
260	270	280	290	300
NDSPVKDEVV	VNDRWGQNCS	CHHGGYYNCE	DKFKPQSLPD	HKWEMCTSID
310	320	330	340	350
KFSWGYRRDM	ALSDVTEESE	IISELVQTVS	LGGNYLLNIG	PTKDGLIVPI
360	370	380	390	400
FQERLLAVGK	WLSINGEAIY	ASKPWRVQWE	KNTTSVWYTS	KGSAVYAIFL
410	420	430	440	450
HWPENGVLNL	ESPITTSTTK	ITMLGIQGDL	KWSTDPDKGL	FISLPQLPPS
460	466
AVPAEFAWTI	KLTGVK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2215 Collapse assay details

Data source: Panorama

Official Gene Symbol: FUCA1
Peptide Modified Sequence: N[+1.0]TTSVWYTSK
Modification Type: Deamidated (N)
Protein - Site of Modification: 382
Peptide - Site of Modification: 1
Peptide Start: 382
Peptide End: 391

CPTAC ID: CPTAC-2215
Peptide Molecular Mass: 1,186.5506
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	17.3	11.2	9.1	16.1	12.2	5.9	23.6	16.6	10.8	15	15	15
y7 (1+)	16	6.7	12.6	17.6	11.6	15.6	23.8	13.4	20.1	15	15	15
y5 (1+)	9.7	6.2	6.7	11.6	7.7	8.3	15.1	9.9	10.7	15	15	15
sum	7.5	4.9	5.5	7	4.8	6.3	10.3	6.9	8.4	15	15	15

FUCA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2215 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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FUCA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2215 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments