BMPR2, CPTAC-2209 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BMPR2	PPH1

Sequence Length (AA)	Molecular Weight (Da)
1038	115201

Protein Name
Bone morphogenetic protein receptor type-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSSLQRPWR	VPWLPWTILL	VSTAAASQNQ	ERLCAFKDPY	QQDLGIGESR
60	70	80	90	100
ISHENGTILC	SKGSTCYGLW	EKSKGDINLV	KQGCWSHIGD	PQECHYEECV
110	120	130	140	150
VTTTPPSIQN	GTYRFCCCST	DLCNVNFTEN	FPPPDTTPLS	PPHSFNRDET
160	170	180	190	200
IIIALASVSV	LAVLIVALCF	GYRMLTGDRK	QGLHSMNMME	AAASEPSLDL
210	220	230	240	250
DNLKLLELIG	RGRYGAVYKG	SLDERPVAVK	VFSFANRQNF	INEKNIYRVP
260	270	280	290	300
LMEHDNIARF	IVGDERVTAD	GRMEYLLVME	YYPNGSLCKY	LSLHTSDWVS
310	320	330	340	350
SCRLAHSVTR	GLAYLHTELP	RGDHYKPAIS	HRDLNSRNVL	VKNDGTCVIS
360	370	380	390	400
DFGLSMRLTG	NRLVRPGEED	NAAISEVGTI	RYMAPEVLEG	AVNLRDCESA
410	420	430	440	450
LKQVDMYALG	LIYWEIFMRC	TDLFPGESVP	EYQMAFQTEV	GNHPTFEDMQ
460	470	480	490	500
VLVSREKQRP	KFPEAWKENS	LAVRSLKETI	EDCWDQDAEA	RLTAQCAEER
510	520	530	540	550
MAELMMIWER	NKSVSPTVNP	MSTAMQNERN	LSHNRRVPKI	GPYPDYSSSS
560	570	580	590	600
YIEDSIHHTD	SIVKNISSEH	SMSSTPLTIG	EKNRNSINYE	RQQAQARIPS
610	620	630	640	650
PETSVTSLST	NTTTTNTTGL	TPSTGMTTIS	EMPYPDETNL	HTTNVAQSIG
660	670	680	690	700
PTPVCLQLTE	EDLETNKLDP	KEVDKNLKES	SDENLMEHSL	KQFSGPDPLS
710	720	730	740	750
STSSSLLYPL	IKLAVEATGQ	QDFTQTANGQ	ACLIPDVLPT	QIYPLPKQQN
760	770	780	790	800
LPKRPTSLPL	NTKNSTKEPR	LKFGSKHKSN	LKQVETGVAK	MNTINAAEPH
810	820	830	840	850
VVTVTMNGVA	GRNHSVNSHA	ATTQYANGTV	LSGQTTNIVT	HRAQEMLQNQ
860	870	880	890	900
FIGEDTRLNI	NSSPDEHEPL	LRREQQAGHD	EGVLDRLVDR	RERPLEGGRT
910	920	930	940	950
NSNNNNSNPC	SEQDVLAQGV	PSTAADPGPS	KPRRAQRPNS	LDLSATNVLD
960	970	980	990	1000
GSSIQIGEST	QDGKSGSGEK	IKKRVKTPYS	LKRWRPSTWV	ISTESLDCEV
1010	1020	1030	1038
NNNGSNRAVH	SKSSTAVYLA	EGGTATTMVS	KDIGMNCL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2209 Collapse assay details

Data source: Panorama

Official Gene Symbol: BMPR2
Peptide Modified Sequence: ISHEN[+1.0]GTILC[+57.0]SK
Modification Type: Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification: 55, 60
Peptide - Site of Modification: 5, 10
Peptide Start: 51
Peptide End: 62

CPTAC ID: CPTAC-2209
Peptide Molecular Mass: 1,358.6500
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	3.8	3.4	2.5	4.7	3.5	3.4	6	4.9	4.2	15	15	15
y5 (1+)	4.1	4.5	3.4	5.4	4.6	3.8	6.8	6.4	5.1	15	15	15
y4 (1+)	4	2.7	2.5	4.2	2.6	2.6	5.8	3.7	3.6	15	15	15
sum	3.3	3	2.1	3.8	2.8	2.3	5	4.1	3.1	15	15	15

BMPR2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2209 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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BMPR2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2209 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments