GAA, CPTAC-2224 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GAA	N/A

Sequence Length (AA)	Molecular Weight (Da)
952	105324

Protein Name
Lysosomal alpha-glucosidase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGVRHPPCSH	RLLAVCALVS	LATAALLGHI	LLHDFLLVPR	ELSGSSPVLE
60	70	80	90	100
ETHPAHQQGA	SRPGPRDAQA	HPGRPRAVPT	QCDVPPNSRF	DCAPDKAITQ
110	120	130	140	150
EQCEARGCCY	IPAKQGLQGA	QMGQPWCFFP	PSYPSYKLEN	LSSSEMGYTA
160	170	180	190	200
TLTRTTPTFF	PKDILTLRLD	VMMETENRLH	FTIKDPANRR	YEVPLETPHV
210	220	230	240	250
HSRAPSPLYS	VEFSEEPFGV	IVRRQLDGRV	LLNTTVAPLF	FADQFLQLST
260	270	280	290	300
SLPSQYITGL	AEHLSPLMLS	TSWTRITLWN	RDLAPTPGAN	LYGSHPFYLA
310	320	330	340	350
LEDGGSAHGV	FLLNSNAMDV	VLQPSPALSW	RSTGGILDVY	IFLGPEPKSV
360	370	380	390	400
VQQYLDVVGY	PFMPPYWGLG	FHLCRWGYSS	TAITRQVVEN	MTRAHFPLDV
410	420	430	440	450
QWNDLDYMDS	RRDFTFNKDG	FRDFPAMVQE	LHQGGRRYMM	IVDPAISSSG
460	470	480	490	500
PAGSYRPYDE	GLRRGVFITN	ETGQPLIGKV	WPGSTAFPDF	TNPTALAWWE
510	520	530	540	550
DMVAEFHDQV	PFDGMWIDMN	EPSNFIRGSE	DGCPNNELEN	PPYVPGVVGG
560	570	580	590	600
TLQAATICAS	SHQFLSTHYN	LHNLYGLTEA	IASHRALVKA	RGTRPFVISR
610	620	630	640	650
STFAGHGRYA	GHWTGDVWSS	WEQLASSVPE	ILQFNLLGVP	LVGADVCGFL
660	670	680	690	700
GNTSEELCVR	WTQLGAFYPF	MRNHNSLLSL	PQEPYSFSEP	AQQAMRKALT
710	720	730	740	750
LRYALLPHLY	TLFHQAHVAG	ETVARPLFLE	FPKDSSTWTV	DHQLLWGEAL
760	770	780	790	800
LITPVLQAGK	AEVTGYFPLG	TWYDLQTVPV	EALGSLPPPP	AAPREPAIHS
810	820	830	840	850
EGQWVTLPAP	LDTINVHLRA	GYIIPLQGPG	LTTTESRQQP	MALAVALTKG
860	870	880	890	900
GEARGELFWD	DGESLEVLER	GAYTQVIFLA	RNNTIVNELV	RVTSEGAGLQ
910	920	930	940	950
LQKVTVLGVA	TAPQQVLSNG	VPVSNFTYSP	DTKVLDICVS	LLMGEQFLVS
952
WC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2224 Collapse assay details

Data source: Panorama

Official Gene Symbol: GAA
Peptide Modified Sequence: QVVEN[+1.0]M[+16.0]TR
Modification Type: Deamidated (N), Oxidation (M)
Protein - Site of Modification: 390, 391
Peptide - Site of Modification: 5, 6
Peptide Start: 386
Peptide End: 393

CPTAC ID: CPTAC-2224
Peptide Molecular Mass: 992.4597
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	32	10.4	18.3	38.2	14.8	22.3	49.8	18.1	28.8	15	15	15
y5 (1+)	17.8	12.6	10.9	20.8	10.3	10.6	27.4	16.3	15.2	15	15	15
y6 (1+)	31.8	5.9	14.8	30.6	13.3	15	44.1	14.5	21.1	15	15	15
sum	9.6	5.9	11.3	11.8	5.1	11.1	15.2	7.8	15.8	15	15	15

GAA

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2224 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

GAA

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2224 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments