Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FGL2 N/A
Sequence Length (AA) Molecular Weight (Da)
439 50229
Protein Name
Fibroleukin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLANWYWLS SAVLATYGFL VVANNETEEI KDERAKDVCP VRLESRGKCE
60 70 80 90 100
EAGECPYQVS LPPLTIQLPK QFSRIEEVFK EVQNLKEIVN SLKKSCQDCK
110 120 130 140 150
LQADDNGDPG RNGLLLPSTG APGEVGDNRV RELESEVNKL SSELKNAKEE
160 170 180 190 200
INVLHGRLEK LNLVNMNNIE NYVDSKVANL TFVVNSLDGK CSKCPSQEQI
210 220 230 240 250
QSRPVQHLIY KDCSDYYAIG KRSSETYRVT PDPKNSSFEV YCDMETMGGG
260 270 280 290 300
WTVLQARLDG STNFTRTWQD YKAGFGNLRR EFWLGNDKIH LLTKSKEMIL
310 320 330 340 350
RIDLEDFNGV ELYALYDQFY VANEFLKYRL HVGNYNGTAG DALRFNKHYN
360 370 380 390 400
HDLKFFTTPD KDNDRYPSGN CGLYYSSGWW FDACLSANLN GKYYHQKYRG
410 420 430 439
VRNGIFWGTW PGVSEAHPGG YKSSFKEAKM MIRPKHFKP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-2214 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGL2
Peptide Modified Sequence
LHVGNYN[+1.0]GTAGDALR
Modification Type
Deamidated (N)
Protein - Site of Modification
336
Peptide - Site of Modification
7
Peptide Start
330
Peptide End
344
CPTAC ID
CPTAC-2214
Peptide Molecular Mass
1,557.7536
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.9 7.9 5.1 11.9 5.7 4.7 16.1 9.7 6.9 15 15 15
y5 (1+) 9.3 5.4 3.7 8.7 4 5.4 12.7 6.7 6.5 15 15 15
y3 (1+) 9.9 6.2 5.4 13.3 6.4 6.5 16.6 8.9 8.5 15 15 15
sum 7.8 4 3.7 8.1 2.2 3.7 11.2 4.6 5.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-2213 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGL2
Peptide Modified Sequence
VAN[+1.0]LTFVVNSLDGK
Modification Type
Deamidated (N)
Protein - Site of Modification
179
Peptide - Site of Modification
3
Peptide Start
177
Peptide End
190
CPTAC ID
CPTAC-2213
Peptide Molecular Mass
1,476.7824
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.9 5.2 3.5 10 8.7 4.4 12.1 10.1 5.6 15 15 15
y8 (1+) 18.5 5.9 7.2 21.5 5.6 7.3 28.4 8.1 10.3 15 15 15
y10 (1+) 14.3 5.8 4.7 14 5.5 6.2 20 8 7.8 15 15 15
sum 10.8 2.3 2.9 9.3 3.2 3.8 14.3 3.9 4.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3269 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGL2
Peptide Sequence
VANLTSVVNSLDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
178
Peptide End
191
CPTAC ID
CPTAC-3269
Peptide Molecular Mass
1,415.7620
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 8.9 6.4 3.7 20.5 13.5 13.4 22.3 14.9 13.9 15 15 15
y10 (1+) 5.8 4.1 3.8 12.8 13.4 14.5 14.1 14 15 15 15 15
y7 (1+) 12.5 7.6 3.9 21.5 15.6 13.8 24.9 17.4 14.3 15 15 15
sum 5.4 2.9 2.3 15.5 12.8 13.6 16.4 13.1 13.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3274 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGL2
Peptide Sequence
VANLTSVVNSLDGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
178
Peptide End
191
CPTAC ID
CPTAC-3274
Peptide Molecular Mass
1,415.7620
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 14.7 4.6 3 14 4.6 3.5 20.3 6.5 4.6 15 15 15
y9 (1+) 13 5.5 4.9 13.9 6.6 4.9 19 8.6 6.9 15 15 15
y7 (1+) 38.8 5.4 4.3 37.9 8.2 4.8 54.2 9.8 6.4 15 15 15
sum 12 3.4 1.9 14 4.9 2.7 18.4 6 3.3 15 15 15

Additional Resources and Comments