GGT1, CPTAC-2217 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GGT1	GGT

Sequence Length (AA)	Molecular Weight (Da)
569	61410

Protein Name
Gamma-glutamyltranspeptidase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKKKLVVLGL	LAVVLVLVIV	GLCLWLPSAS	KEPDNHVYTR	AAVAADAKQC
60	70	80	90	100
SKIGRDALRD	GGSAVDAAIA	ALLCVGLMNA	HSMGIGGGLF	LTIYNSTTRK
110	120	130	140	150
AEVINAREVA	PRLAFATMFN	SSEQSQKGGL	SVAVPGEIRG	YELAHQRHGR
160	170	180	190	200
LPWARLFQPS	IQLARQGFPV	GKGLAAALEN	KRTVIEQQPV	LCEVFCRDRK
210	220	230	240	250
VLREGERLTL	PQLADTYETL	AIEGAQAFYN	GSLTAQIVKD	IQAAGGIVTA
260	270	280	290	300
EDLNNYRAEL	IEHPLNISLG	DVVLYMPSAP	LSGPVLALIL	NILKGYNFSR
310	320	330	340	350
ESVESPEQKG	LTYHRIVEAF	RFAYAKRTLL	GDPKFVDVTE	VVRNMTSEFF
360	370	380	390	400
AAQLRAQISD	DTTHPISYYK	PEFYTPDDGG	TAHLSVVAED	GSAVSATSTI
410	420	430	440	450
NLYFGSKVRS	PVSGILFNNE	MDDFSSPSIT	NEFGVPPSPA	NFIQPGKQPL
460	470	480	490	500
SSMCPTIMVG	QDGQVRMVVG	AAGGTQITTA	TALAIIYNLW	FGYDVKRAVE
510	520	530	540	550
EPRLHNQLLP	NVTTVERNID	QAVTAALETR	HHHTQIASTF	IAVVQAIVRT
560	569
AGGWAAASDS	RKGGEPAGY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2217 Collapse assay details

Data source: Panorama

Official Gene Symbol: GGT1
Peptide Modified Sequence: LHNQLLPN[+1.0]VTTVER
Modification Type: Deamidated (N)
Protein - Site of Modification: 511
Peptide - Site of Modification: 8
Peptide Start: 504
Peptide End: 517

CPTAC ID: CPTAC-2217
Peptide Molecular Mass: 1,633.8788
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	4.2	1.8	2.2	4.5	2.1	2	6.2	2.8	3	15	15	15
b5 (1+)	4.6	2.3	3	4.5	2.1	3.4	6.4	3.1	4.5	15	15	15
b6 (1+)	5.4	1.9	3.1	5	1.7	2.4	7.4	2.5	3.9	15	15	15
sum	4	1.5	2	3.6	1.6	1.5	5.4	2.2	2.5	15	15	15

GGT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2217 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GGT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2217 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments