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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ATP1B3 N/A
Sequence Length (AA) Molecular Weight (Da)
279 31513
Protein Name
Sodium/potassium-transporting ATPase subunit beta-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTKNEKKSLN QSLAEWKLFI YNPTTGEFLG RTAKSWGLIL LFYLVFYGFL
60 70 80 90 100
AALFSFTMWV MLQTLNDEVP KYRDQIPSPG LMVFPKPVTA LEYTFSRSDP
110 120 130 140 150
TSYAGYIEDL KKFLKPYTLE EQKNLTVCPD GALFEQKGPV YVACQFPISL
160 170 180 190 200
LQACSGMNDP DFGYSQGNPC ILVKMNRIIG LKPEGVPRID CVSKNEDIPN
210 220 230 240 250
VAVYPHNGMI DLKYFPYYGK KLHVGYLQPL VAVQVSFAPN NTGKEVTVEC
260 270 279
KIDGSANLKS QDDRDKFLGR VMFKITARA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2206 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATP1B3
Peptide Modified Sequence
LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
Modification Type
Deamidated (N)
Protein - Site of Modification
240
Peptide - Site of Modification
19
Peptide Start
222
Peptide End
244
CPTAC ID
CPTAC-2206
Peptide Molecular Mass
2,452.3114
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 23.5 6.6 6.3 28.8 6.1 7.8 37.2 9 10 15 15 15
y6 (1+) 16.5 4.4 3.6 19 4.1 3.6 25.2 6 5.1 15 15 15
b5 (1+) 23.7 8.4 4.5 24.7 7.5 6.2 34.2 11.3 7.7 15 15 15
sum 18.6 2.6 1.7 21.3 2.7 2 28.3 3.7 2.6 15 15 15

Additional Resources and Comments