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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LTBR D12S370, TNFCR, TNFR3, TNFRSF3
Sequence Length (AA) Molecular Weight (Da)
435 46709
Protein Name
Tumor necrosis factor receptor superfamily member 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLLPWATSAP GLAWGPLVLG LFGLLAASQP QAVPPYASEN QTCRDQEKEY
60 70 80 90 100
YEPQHRICCS RCPPGTYVSA KCSRIRDTVC ATCAENSYNE HWNYLTICQL
110 120 130 140 150
CRPCDPVMGL EEIAPCTSKR KTQCRCQPGM FCAAWALECT HCELLSDCPP
160 170 180 190 200
GTEAELKDEV GKGNNHCVPC KAGHFQNTSS PSARCQPHTR CENQGLVEAA
210 220 230 240 250
PGTAQSDTTC KNPLEPLPPE MSGTMLMLAV LLPLAFFLLL ATVFSCIWKS
260 270 280 290 300
HPSLCRKLGS LLKRRPQGEG PNPVAGSWEP PKAHPYFPDL VQPLLPISGD
310 320 330 340 350
VSPVSTGLPA APVLEAGVPQ QQSPLDLTRE PQLEPGEQSQ VAHGTNGIHV
360 370 380 390 400
TGGSMTITGN IYIYNGPVLG GPPGPGDLPA TPEPPYPIPE EGDPGPPGLS
410 420 430 435
TPHQEDGKAW HLAETEHCGA TPSNRGPRNQ FITHD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2234 Collapse assay details

Data source: Panorama

Official Gene Symbol
LTBR
Peptide Modified Sequence
AGHFQN[+1.0]TSSPSAR
Modification Type
Deamidated (N)
Protein - Site of Modification
177
Peptide - Site of Modification
6
Peptide Start
172
Peptide End
184
CPTAC ID
CPTAC-2234
Peptide Molecular Mass
1,359.6167
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.2 7.6 7 10.1 7.4 6.7 12.4 10.6 9.7 15 15 15
y4 (1+) 12 4.6 4.2 10.4 4.9 4.1 15.9 6.7 5.9 15 15 15
y6 (1+) 13.8 10.7 7.5 16.4 13.2 8 21.4 17 11 15 15 15
sum 9.4 3.5 3.6 8.6 3.9 3.9 12.7 5.2 5.3 15 15 15


Additional Resources and Comments