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Overview Data source: UniProt

Official Gene Symbol Other Aliases
B3GNT2 B3GALT7, B3GNT1
Sequence Length (AA) Molecular Weight (Da)
397 46022
Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSVGRRRIKL LGILMMANVF IYFIMEVSKS SSQEKNGKGE VIIPKEKFWK
60 70 80 90 100
ISTPPEAYWN REQEKLNRQY NPILSMLTNQ TGEAGRLSNI SHLNYCEPDL
110 120 130 140 150
RVTSVVTGFN NLPDRFKDFL LYLRCRNYSL LIDQPDKCAK KPFLLLAIKS
160 170 180 190 200
LTPHFARRQA IRESWGQESN AGNQTVVRVF LLGQTPPEDN HPDLSDMLKF
210 220 230 240 250
ESEKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDTEF VFKGDDDVFV
260 270 280 290 300
NTHHILNYLN SLSKTKAKDL FIGDVIHNAG PHRDKKLKYY IPEVVYSGLY
310 320 330 340 350
PPYAGGGGFL YSGHLALRLY HITDQVHLYP IDDVYTGMCL QKLGLVPEKH
360 370 380 390 397
KGFRTFDIEE KNKNNICSYV DLMLVHSRKP QEMIDIWSQL QSAHLKC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2208 Collapse assay details

Data source: Panorama

Official Gene Symbol
B3GNT2
Peptide Modified Sequence
DTFFN[+1.0]LSLK
Modification Type
Deamidated (N)
Protein - Site of Modification
219
Peptide - Site of Modification
5
Peptide Start
215
Peptide End
223
CPTAC ID
CPTAC-2208
Peptide Molecular Mass
1,084.5441
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 15.4 4.4 6.2 16.5 4.7 6.4 22.6 6.4 8.9 15 15 15
y5 (1+) 18 10.1 6.7 20.9 10.4 5.1 27.6 14.5 8.4 15 15 15
y7 (1+) 18.1 8.5 5.7 22.2 7.9 8.2 28.6 11.6 10 15 15 15
sum 12.3 4.5 2.6 14.9 3.8 2.8 19.3 5.9 3.8 15 15 15

Additional Resources and Comments