AOC1, CPTAC-2204 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AOC1	ABP1, DAO1

Sequence Length (AA)	Molecular Weight (Da)
751	85378

Protein Name
Amiloride-sensitive amine oxidase [copper-containing]

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPALGWAVAA	ILMLQTAMAE	PSPGTLPRKA	GVFSDLSNQE	LKAVHSFLWS
60	70	80	90	100
KKELRLQPSS	TTTMAKNTVF	LIEMLLPKKY	HVLRFLDKGE	RHPVREARAV
110	120	130	140	150
IFFGDQEHPN	VTEFAVGPLP	GPCYMRALSP	RPGYQSSWAS	RPISTAEYAL
160	170	180	190	200
LYHTLQEATK	PLHQFFLNTT	GFSFQDCHDR	CLAFTDVAPR	GVASGQRRSW
210	220	230	240	250
LIIQRYVEGY	FLHPTGLELL	VDHGSTDAGH	WAVEQVWYNG	KFYGSPEELA
260	270	280	290	300
RKYADGEVDV	VVLEDPLPGG	KGHDSTEEPP	LFSSHKPRGD	FPSPIHVSGP
310	320	330	340	350
RLVQPHGPRF	RLEGNAVLYG	GWSFAFRLRS	SSGLQVLNVH	FGGERIAYEV
360	370	380	390	400
SVQEAVALYG	GHTPAGMQTK	YLDVGWGLGS	VTHELAPGID	CPETATFLDT
410	420	430	440	450
FHYYDADDPV	HYPRALCLFE	MPTGVPLRRH	FNSNFKGGFN	FYAGLKGQVL
460	470	480	490	500
VLRTTSTVYN	YDYIWDFIFY	PNGVMEAKMH	ATGYVHATFY	TPEGLRHGTR
510	520	530	540	550
LHTHLIGNIH	THLVHYRVDL	DVAGTKNSFQ	TLQMKLENIT	NPWSPRHRVV
560	570	580	590	600
QPTLEQTQYS	WERQAAFRFK	RKLPKYLLFT	SPQENPWGHK	RTYRLQIHSM
610	620	630	640	650
ADQVLPPGWQ	EEQAITWARY	PLAVTKYRES	ELCSSSIYHQ	NDPWHPPVVF
660	670	680	690	700
EQFLHNNENI	ENEDLVAWVT	VGFLHIPHSE	DIPNTATPGN	SVGFLLRPFN
710	720	730	740	750
FFPEDPSLAS	RDTVIVWPRD	NGPNYVQRWI	PEDRDCSMPP	PFSYNGTYRP
751
V

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2204 Collapse assay details

Data source: Panorama

Official Gene Symbol: AOC1
Peptide Modified Sequence: LEN[+1.0]ITNPWSPR
Modification Type: Deamidated (N)
Protein - Site of Modification: 538
Peptide - Site of Modification: 3
Peptide Start: 536
Peptide End: 546

CPTAC ID: CPTAC-2204
Peptide Molecular Mass: 1,326.6568
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	49.6	17.2	42.3	59.9	30.2	45.8	77.8	34.8	62.3	15	15	15
y7 (1+)	24.4	21.2	17.6	29.3	19.6	21.3	38.1	28.9	27.6	15	15	15
y5 (1+)	42.7	19.9	18.4	40.9	20.2	14.7	59.1	28.4	23.6	15	15	15
sum	32.2	8.6	12.5	29.6	11.5	10.2	43.7	14.4	16.1	15	15	15

AOC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2204 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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AOC1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2204 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments