GNPTAB, CPTAC-2218 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GNPTAB	GNPTA, KIAA1208

Sequence Length (AA)	Molecular Weight (Da)
1256	143622

Protein Name
N-acetylglucosamine-1-phosphotransferase subunits alpha/beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLFKLLQRQT	YTCLSHRYGL	YVCFLGVVVT	IVSAFQFGEV	VLEWSRDQYH
60	70	80	90	100
VLFDSYRDNI	AGKSFQNRLC	LPMPIDVVYT	WVNGTDLELL	KELQQVREQM
110	120	130	140	150
EEEQKAMREI	LGKNTTEPTK	KSEKQLECLL	THCIKVPMLV	LDPALPANIT
160	170	180	190	200
LKDLPSLYPS	FHSASDIFNV	AKPKNPSTNV	SVVVFDSTKD	VEDAHSGLLK
210	220	230	240	250
GNSRQTVWRG	YLTTDKEVPG	LVLMQDLAFL	SGFPPTFKET	NQLKTKLPEN
260	270	280	290	300
LSSKVKLLQL	YSEASVALLK	LNNPKDFQEL	NKQTKKNMTI	DGKELTISPA
310	320	330	340	350
YLLWDLSAIS	QSKQDEDISA	SRFEDNEELR	YSLRSIERHA	PWVRNIFIVT
360	370	380	390	400
NGQIPSWLNL	DNPRVTIVTH	QDVFRNLSHL	PTFSSPAIES	HIHRIEGLSQ
410	420	430	440	450
KFIYLNDDVM	FGKDVWPDDF	YSHSKGQKVY	LTWPVPNCAE	GCPGSWIKDG
460	470	480	490	500
YCDKACNNSA	CDWDGGDCSG	NSGGSRYIAG	GGGTGSIGVG	QPWQFGGGIN
510	520	530	540	550
SVSYCNQGCA	NSWLADKFCD	QACNVLSCGF	DAGDCGQDHF	HELYKVILLP
560	570	580	590	600
NQTHYIIPKG	ECLPYFSFAE	VAKRGVEGAY	SDNPIIRHAS	IANKWKTIHL
610	620	630	640	650
IMHSGMNATT	IHFNLTFQNT	NDEEFKMQIT	VEVDTREGPK	LNSTAQKGYE
660	670	680	690	700
NLVSPITLLP	EAEILFEDIP	KEKRFPKFKR	HDVNSTRRAQ	EEVKIPLVNI
710	720	730	740	750
SLLPKDAQLS	LNTLDLQLEH	GDITLKGYNL	SKSALLRSFL	MNSQHAKIKN
760	770	780	790	800
QAIITDETND	SLVAPQEKQV	HKSILPNSLG	VSERLQRLTF	PAVSVKVNGH
810	820	830	840	850
DQGQNPPLDL	ETTARFRVET	HTQKTIGGNV	TKEKPPSLIV	PLESQMTKEK
860	870	880	890	900
KITGKEKENS	RMEENAENHI	GVTEVLLGRK	LQHYTDSYLG	FLPWEKKKYF
910	920	930	940	950
QDLLDEEESL	KTQLAYFTDS	KNTGRQLKDT	FADSLRYVNK	ILNSKFGFTS
960	970	980	990	1000
RKVPAHMPHM	IDRIVMQELQ	DMFPEEFDKT	SFHKVRHSED	MQFAFSYFYY
1010	1020	1030	1040	1050
LMSAVQPLNI	SQVFDEVDTD	QSGVLSDREI	RTLATRIHEL	PLSLQDLTGL
1060	1070	1080	1090	1100
EHMLINCSKM	LPADITQLNN	IPPTQESYYD	PNLPPVTKSL	VTNCKPVTDK
1110	1120	1130	1140	1150
IHKAYKDKNK	YRFEIMGEEE	IAFKMIRTNV	SHVVGQLDDI	RKNPRKFVCL
1160	1170	1180	1190	1200
NDNIDHNHKD	AQTVKAVLRD	FYESMFPIPS	QFELPREYRN	RFLHMHELQE
1210	1220	1230	1240	1250
WRAYRDKLKF	WTHCVLATLI	MFTIFSFFAE	QLIALKRKIF	PRRRIHKEAS
1256
PNRIRV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2218 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNPTAB
Peptide Modified Sequence: TN[+1.0]VSHVVGQLDDIR
Modification Type: Deamidated (N)
Protein - Site of Modification: 1129
Peptide - Site of Modification: 2
Peptide Start: 1128
Peptide End: 1141

CPTAC ID: CPTAC-2218
Peptide Molecular Mass: 1,552.7845
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	20.7	14.5	14.7	22.2	16.9	22.7	30.4	22.3	27	15	15	15
y5 (1+)	16.3	20.7	17.9	25	25.4	24.1	29.8	32.8	30	15	15	15
y7 (1+)	23.6	22	30.8	22.2	32.7	28.1	32.4	39.4	41.7	15	15	15
sum	16.3	8.7	7.5	15.2	14.2	13	22.3	16.7	15	15	15	15

GNPTAB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2218 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GNPTAB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2218 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments