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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PON1 PON
Sequence Length (AA) Molecular Weight (Da)
355 39731
Protein Name
Serum paraoxonase/arylesterase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAKLIALTLL GMGLALFRNH QSSYQTRLNA LREVQPVELP NCNLVKGIET
60 70 80 90 100
GSEDLEILPN GLAFISSGLK YPGIKSFNPN SPGKILLMDL NEEDPTVLEL
110 120 130 140 150
GITGSKFDVS SFNPHGISTF TDEDNAMYLL VVNHPDAKST VELFKFQEEE
160 170 180 190 200
KSLLHLKTIR HKLLPNLNDI VAVGPEHFYG TNDHYFLDPY LQSWEMYLGL
210 220 230 240 250
AWSYVVYYSP SEVRVVAEGF DFANGINISP DGKYVYIAEL LAHKIHVYEK
260 270 280 290 300
HANWTLTPLK SLDFNTLVDN ISVDPETGDL WVGCHPNGMK IFFYDSENPP
310 320 330 340 350
ASEVLRIQNI LTEEPKVTQV YAENGTVLQG STVASVYKGK LLIGTVFHKA
355
LYCEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2233 Collapse assay details

Data source: Panorama

Official Gene Symbol
PON1
Peptide Modified Sequence
HAN[+1.0]WTLTPLK
Modification Type
Deamidated (N)
Protein - Site of Modification
253
Peptide - Site of Modification
3
Peptide Start
251
Peptide End
260
CPTAC ID
CPTAC-2233
Peptide Molecular Mass
1,180.6241
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.3 1.9 3.1 4.5 2.5 1.9 6.2 3.1 3.6 15 15 15
y4 (1+) 4 1.7 1.2 4.8 1.8 1.6 6.2 2.5 2 15 15 15
y3 (1+) 4.4 2.3 1.3 4.8 2.1 1.6 6.5 3.1 2.1 15 15 15
sum 3.3 1.1 1.3 4.1 1.5 0.9 5.3 1.9 1.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2695 Collapse assay details

Data source: Panorama

Official Gene Symbol
PON1
Peptide Sequence
IFFYDSENPPASEVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
291
Peptide End
306
CPTAC ID
non-CPTAC-2695
Peptide Molecular Mass
1,882.9101
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.5 5.7 8.1 11.5 8.2 8 15.6 10 11.4 15 15 15
b2 (1+) 9 17.1 5.4 11.7 22 6.2 14.8 27.9 8.2 15 15 15
b8 (1+) 14.7 6.7 6.3 22.9 7.1 5.7 27.2 9.8 8.5 15 15 15
y7 (1+) 12.6 9.6 7.2 17.6 10.7 7.6 21.6 14.4 10.5 15 15 15
y9 (1+) 9.5 17.5 4.8 19.5 19.5 7.1 21.7 26.2 8.6 15 15 15
sum 6.5 5.3 4.5 10 8.7 5.1 11.9 10.2 6.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3380 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pon1
Peptide Sequence
YVYIAELLAHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
234
Peptide End
244
CPTAC ID
non-CPTAC-3380
Peptide Molecular Mass
1,318.7285
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.4 1.7 3.4 14.7 4.2 4.9 15.1 4.5 6 15 15 15
y9 (2+) 10.6 5.1 6.4 13.3 6.7 6.7 17 8.4 9.3 15 15 15
y7 (1+) 8.5 4.7 4.3 14.5 6.3 5.5 16.8 7.9 7 15 15 15
y8 (1+) 14.6 30 16.9 16 46.5 43.6 21.7 55.3 46.8 15 15 15
y9 (1+) 6.3 4.8 2.9 13 6.4 3.7 14.4 8 4.7 15 15 15
sum 3 7.9 3.7 12.9 13.6 10.7 13.2 15.7 11.3 15 15 15


Additional Resources and Comments