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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

GPLD1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GPLD1 PIGPLD1
Sequence Length (AA) Molecular Weight (Da)
840 92336
Protein Name
Phosphatidylinositol-glycan-specific phospholipase D
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSAFRLWPGL LIMLGSLCHR GSPCGLSTHV EIGHRALEFL QLHNGRVNYR
60 70 80 90 100
ELLLEHQDAY QAGIVFPDCF YPSICKGGKF HDVSESTHWT PFLNASVHYI
110 120 130 140 150
RENYPLPWEK DTEKLVAFLF GITSHMAADV SWHSLGLEQG FLRTMGAIDF
160 170 180 190 200
HGSYSEAHSA GDFGGDVLSQ FEFNFNYLAR RWYVPVKDLL GIYEKLYGRK
210 220 230 240 250
VITENVIVDC SHIQFLEMYG EMLAVSKLYP TYSTKSPFLV EQFQEYFLGG
260 270 280 290 300
LDDMAFWSTN IYHLTSFMLE NGTSDCNLPE NPLFIACGGQ QNHTQGSKMQ
310 320 330 340 350
KNDFHRNLTT SLTESVDRNI NYTERGVFFS VNSWTPDSMS FIYKALERNI
360 370 380 390 400
RTMFIGGSQL SQKHVSSPLA SYFLSFPYAR LGWAMTSADL NQDGHGDLVV
410 420 430 440 450
GAPGYSRPGH IHIGRVYLIY GNDLGLPPVD LDLDKEAHRI LEGFQPSGRF
460 470 480 490 500
GSALAVLDFN VDGVPDLAVG APSVGSEQLT YKGAVYVYFG SKQGGMSSSP
510 520 530 540 550
NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF
560 570 580 590 600
YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP
610 620 630 640 650
TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS
660 670 680 690 700
LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD
710 720 730 740 750
AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL
760 770 780 790 800
IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF
810 820 830 840
GSSLITVRSK AKNQVVIAAG RSSLGARLSG ALHVYSLGSD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2231 Collapse assay details

Data source: Panorama

Official Gene Symbol
GPLD1
Peptide Modified Sequence
N[+1.0]LTTSLTESVDR
Modification Type
Deamidated (N)
Protein - Site of Modification
307
Peptide - Site of Modification
1
Peptide Start
307
Peptide End
318
CPTAC ID
CPTAC-2231
Peptide Molecular Mass
1,335.6518
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 30.1 8 16.7 29.1 10.2 15.7 41.9 13 22.9 15 15 15
y9 (1+) 32.4 19 14.2 41.9 19.1 15.4 53 26.9 20.9 15 15 15
y8 (1+) 41.8 9.8 13.4 46.5 10.7 12.1 62.5 14.5 18.1 15 15 15
sum 18.6 4.3 9.2 18.6 6 10 26.3 7.4 13.6 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3523 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gpld1
Peptide Sequence
FGSSLVSVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
797
Peptide End
805
CPTAC ID
non-CPTAC-3523
Peptide Molecular Mass
950.5185
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 4.5 3.7 7.3 13.1 5.8 5.8 13.9 6.9 9.3 15 15 15
y4 (1+) 8.2 6.3 3 8.4 7.2 8.1 11.7 9.6 8.6 15 15 15
y7 (1+) 9 5.3 4.6 16.2 10.7 7.5 18.5 11.9 8.8 15 15 15
y8 (1+) 6.6 2 3.9 20.8 4.6 7.3 21.8 5 8.3 15 15 15
b2 (1+) 5.7 5.1 5.8 14.7 7.8 8.4 15.8 9.3 10.2 15 15 15
sum 3.7 2.1 2.1 12.6 5.5 6.3 13.1 5.9 6.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3665 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gpld1
Peptide Sequence
LGTSLSSGYVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
643
Peptide End
653
CPTAC ID
non-CPTAC-3665
Peptide Molecular Mass
1,138.5982
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.4 3.8 4.1 14.8 7.3 7.5 16.1 8.2 8.5 15 15 15
y4 (1+) 22.6 6.5 4.5 27.1 10 8 35.3 11.9 9.2 15 15 15
y5 (1+) 15.1 3.4 5.5 17.4 8.8 7.9 23 9.4 9.6 15 15 15
y6 (1+) 7.5 3.2 4.9 18.3 6.2 6.7 19.8 7 8.3 15 15 15
y7 (1+) 7.3 4.4 4.1 18.1 6.9 9 19.5 8.2 9.9 15 15 15
sum 4.7 1.7 2.3 14.5 6.1 6.3 15.2 6.3 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments