ADAMTS12, CPTAC-2207 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADAMTS12	N/A

Sequence Length (AA)	Molecular Weight (Da)
1594	177676

Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 12

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPCAQRSWLA	NLSVVAQLLN	FGALCYGRQP	QPGPVRFPDR	RQEHFIKGLP
60	70	80	90	100
EYHVVGPVRV	DASGHFLSYG	LHYPITSSRR	KRDLDGSEDW	VYYRISHEEK
110	120	130	140	150
DLFFNLTVNQ	GFLSNSYIME	KRYGNLSHVK	MMASSAPLCH	LSGTVLQQGT
160	170	180	190	200
RVGTAALSAC	HGLTGFFQLP	HGDFFIEPVK	KHPLVEGGYH	PHIVYRRQKV
210	220	230	240	250
PETKEPTCGL	KDSVNISQKQ	ELWREKWERH	NLPSRSLSRR	SISKERWVET
260	270	280	290	300
LVVADTKMIE	YHGSENVESY	ILTIMNMVTG	LFHNPSIGNA	IHIVVVRLIL
310	320	330	340	350
LEEEEQGLKI	VHHAEKTLSS	FCKWQKSINP	KSDLNPVHHD	VAVLLTRKDI
360	370	380	390	400
CAGFNRPCET	LGLSHLSGMC	QPHRSCNINE	DSGLPLAFTI	AHELGHSFGI
410	420	430	440	450
QHDGKENDCE	PVGRHPYIMS	RQLQYDPTPL	TWSKCSEEYI	TRFLDRGWGF
460	470	480	490	500
CLDDIPKKKG	LKSKVIAPGV	IYDVHHQCQL	QYGPNATFCQ	EVENVCQTLW
510	520	530	540	550
CSVKGFCRSK	LDAAADGTQC	GEKKWCMAGK	CITVGKKPES	IPGGWGRWSP
560	570	580	590	600
WSHCSRTCGA	GVQSAERLCN	NPEPKFGGKY	CTGERKRYRL	CNVHPCRSEA
610	620	630	640	650
PTFRQMQCSE	FDTVPYKNEL	YHWFPIFNPA	HPCELYCRPI	DGQFSEKMLD
660	670	680	690	700
AVIDGTPCFE	GGNSRNVCIN	GICKMVGCDY	EIDSNATEDR	CGVCLGDGSS
710	720	730	740	750
CQTVRKMFKQ	KEGSGYVDIG	LIPKGARDIR	VMEIEGAGNF	LAIRSEDPEK
760	770	780	790	800
YYLNGGFIIQ	WNGNYKLAGT	VFQYDRKGDL	EKLMATGPTN	ESVWIQLLFQ
810	820	830	840	850
VTNPGIKYEY	TIQKDGLDND	VEQQMYFWQY	GHWTECSVTC	GTGIRRQTAH
860	870	880	890	900
CIKKGRGMVK	ATFCDPETQP	NGRQKKCHEK	ACPPRWWAGE	WEACSATCGP
910	920	930	940	950
HGEKKRTVLC	IQTMVSDEQA	LPPTDCQHLL	KPKTLLSCNR	DILCPSDWTV
960	970	980	990	1000
GNWSECSVSC	GGGVRIRSVT	CAKNHDEPCD	VTRKPNSRAL	CGLQQCPSSR
1010	1020	1030	1040	1050
RVLKPNKGTI	SNGKNPPTLK	PVPPPTSRPR	MLTTPTGPES	MSTSTPAISS
1060	1070	1080	1090	1100
PSPTTASKEG	DLGGKQWQDS	STQPELSSRY	LISTGSTSQP	ILTSQSLSIQ
1110	1120	1130	1140	1150
PSEENVSSSD	TGPTSEGGLV	ATTTSGSGLS	SSRNPITWPV	TPFYNTLTKG
1160	1170	1180	1190	1200
PEMEIHSGSG	EEREQPEDKD	ESNPVIWTKI	RVPGNDAPVE	STEMPLAPPL
1210	1220	1230	1240	1250
TPDLSRESWW	PPFSTVMEGL	LPSQRPTTSE	TGTPRVEGMV	TEKPANTLLP
1260	1270	1280	1290	1300
LGGDHQPEPS	GKTANRNHLK	LPNNMNQTKS	SEPVLTEEDA	TSLITEGFLL
1310	1320	1330	1340	1350
NASNYKQLTN	GHGSAHWIVG	NWSECSTTCG	LGAYWRRVEC	STQMDSDCAA
1360	1370	1380	1390	1400
IQRPDPAKRC	HLRPCAGWKV	GNWSKCSRNC	SGGFKIREIQ	CVDSRDHRNL
1410	1420	1430	1440	1450
RPFHCQFLAG	IPPPLSMSCN	PEPCEAWQVE	PWSQCSRSCG	GGVQERGVFC
1460	1470	1480	1490	1500
PGGLCDWTKR	PTSTMSCNEH	LCCHWATGNW	DLCSTSCGGG	FQKRTVQCVP
1510	1520	1530	1540	1550
SEGNKTEDQD	QCLCDHKPRP	PEFKKCNQQA	CKKSADLLCT	KDKLSASFCQ
1560	1570	1580	1590	1594
TLKAMKKCSV	PTVRAECCFS	CPQTHITHTQ	RQRRQRLLQK	SKEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2207 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADAMTS12
Peptide Modified Sequence: VGN[+1.0]WSK
Modification Type: Deamidated (N)
Protein - Site of Modification: 1372
Peptide - Site of Modification: 3
Peptide Start: 1370
Peptide End: 1375

CPTAC ID: CPTAC-2207
Peptide Molecular Mass: 690.3337
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	12.4	15.4	16.6	15.2	18	12.4	19.6	23.7	20.7	15	15	15
y2 (1+)	13	8.2	6.4	11.6	8.7	7.9	17.4	12	10.2	15	15	15
y5 (1+)	8.5	3.2	2.2	8.8	4.8	1.9	12.2	5.8	2.9	15	15	15
sum	7.4	3.4	1.7	8.1	4.3	2.1	11	5.5	2.7	15	15	15

ADAMTS12

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2207 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ADAMTS12

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2207 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments