CDCP1, CPTAC-2211 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDCP1	TRASK

Sequence Length (AA)	Molecular Weight (Da)
836	92932

Protein Name
CUB domain-containing protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGLNCGVSI	ALLGVLLLGA	ARLPRGAEAF	EIALPRESNI	TVLIKLGTPT
60	70	80	90	100
LLAKPCYIVI	SKRHITMLSI	KSGERIVFTF	SCQSPENHFV	IEIQKNIDCM
110	120	130	140	150
SGPCPFGEVQ	LQPSTSLLPT	LNRTFIWDVK	AHKSIGLELQ	FSIPRLRQIG
160	170	180	190	200
PGESCPDGVT	HSISGRIDAT	VVRIGTFCSN	GTVSRIKMQE	GVKMALHLPW
210	220	230	240	250
FHPRNVSGFS	IANRSSIKRL	CIIESVFEGE	GSATLMSANY	PEGFPEDELM
260	270	280	290	300
TWQFVVPAHL	RASVSFLNFN	LSNCERKEER	VEYYIPGSTT	NPEVFKLEDK
310	320	330	340	350
QPGNMAGNFN	LSLQGCDQDA	QSPGILRLQF	QVLVQHPQNE	SNKIYVVDLS
360	370	380	390	400
NERAMSLTIE	PRPVKQSRKF	VPGCFVCLES	RTCSSNLTLT	SGSKHKISFL
410	420	430	440	450
CDDLTRLWMN	VEKTISCTDH	RYCQRKSYSL	QVPSDILHLP	VELHDFSWKL
460	470	480	490	500
LVPKDRLSLV	LVPAQKLQQH	THEKPCNTSF	SYLVASAIPS	QDLYFGSFCP
510	520	530	540	550
GGSIKQIQVK	QNISVTLRTF	APSFQQEASR	QGLTVSFIPY	FKEEGVFTVT
560	570	580	590	600
PDTKSKVYLR	TPNWDRGLPS	LTSVSWNISV	PRDQVACLTF	FKERSGVVCQ
610	620	630	640	650
TGRAFMIIQE	QRTRAEEIFS	LDEDVLPKPS	FHHHSFWVNI	SNCSPTSGKQ
660	670	680	690	700
LDLLFSVTLT	PRTVDLTVIL	IAAVGGGVLL	LSALGLIICC	VKKKKKKTNK
710	720	730	740	750
GPAVGIYNDN	INTEMPRQPK	KFQKGRKDND	SHVYAVIEDT	MVYGHLLQDS
760	770	780	790	800
SGSFLQPEVD	TYRPFQGTMG	VCPPSPPTIC	SRAPTAKLAT	EEPPPRSPPE
810	820	830	836
SESEPYTFSH	PNNGDVSSKD	TDIPLLNTQE	PMEPAE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-2211 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDCP1
Peptide Modified Sequence: ESN[+1.0]ITVLIK
Modification Type: Deamidated (N)
Protein - Site of Modification: 39
Peptide - Site of Modification: 3
Peptide Start: 37
Peptide End: 45

CPTAC ID: CPTAC-2211
Peptide Molecular Mass: 1,016.5754
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	19.7	7.6	3.9	27.3	6.9	4.1	33.7	10.3	5.7	15	15	15
y5 (1+)	12.3	3.6	2.6	5.9	3.8	2.8	13.6	5.2	3.8	15	15	15
y7 (1+)	30	6.8	12.3	41.6	8.8	11	51.3	11.1	16.5	15	15	15
sum	10.2	3.6	2.2	6	4.1	2.1	11.8	5.5	3	15	15	15

CDCP1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2211 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CDCP1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2211 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments