P2RX7, CPTAC-2229 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
P2RX7	N/A

Sequence Length (AA)	Molecular Weight (Da)
595	68585

Protein Name
P2X purinoceptor 7

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPACCSCSDV	FQYETNKVTR	IQSMNYGTIK	WFFHVIIFSY	VCFALVSDKL
60	70	80	90	100
YQRKEPVISS	VHTKVKGIAE	VKEEIVENGV	KKLVHSVFDT	ADYTFPLQGN
110	120	130	140	150
SFFVMTNFLK	TEGQEQRLCP	EYPTRRTLCS	SDRGCKKGWM	DPQSKGIQTG
160	170	180	190	200
RCVVYEGNQK	TCEVSAWCPI	EAVEEAPRPA	LLNSAENFTV	LIKNNIDFPG
210	220	230	240	250
HNYTTRNILP	GLNITCTFHK	TQNPQCPIFR	LGDIFRETGD	NFSDVAIQGG
260	270	280	290	300
IMGIEIYWDC	NLDRWFHHCR	PKYSFRRLDD	KTTNVSLYPG	YNFRYAKYYK
310	320	330	340	350
ENNVEKRTLI	KVFGIRFDIL	VFGTGGKFDI	IQLVVYIGST	LSYFGLAAVF
360	370	380	390	400
IDFLIDTYSS	NCCRSHIYPW	CKCCQPCVVN	EYYYRKKCES	IVEPKPTLKY
410	420	430	440	450
VSFVDESHIR	MVNQQLLGRS	LQDVKGQEVP	RPAMDFTDLS	RLPLALHDTP
460	470	480	490	500
PIPGQPEEIQ	LLRKEATPRS	RDSPVWCQCG	SCLPSQLPES	HRCLEELCCR
510	520	530	540	550
KKPGACITTS	ELFRKLVLSR	HVLQFLLLYQ	EPLLALDVDS	TNSRLRHCAY
560	570	580	590	595
RCYATWRFGS	QDMADFAILP	SCCRWRIRKE	FPKSEGQYSG	FKSPY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-2229 Collapse assay details

Data source: Panorama

Official Gene Symbol: P2RX7
Peptide Modified Sequence: TTN[+1.0]VSLYPGYNFR
Modification Type: Deamidated (N)
Protein - Site of Modification: 284
Peptide - Site of Modification: 3
Peptide Start: 282
Peptide End: 294

CPTAC ID: CPTAC-2229
Peptide Molecular Mass: 1,531.7307
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	28.8	15.5	12.6	29.7	17.2	14	41.4	23.2	18.8	15	15	15
y6 (1+)	12.1	4.3	10	17	8.5	11.6	20.9	9.5	15.3	15	15	15
y9 (1+)	27.1	14.9	15.7	27.5	21.1	18.8	38.6	25.8	24.5	15	15	15
sum	12.2	4.5	6.4	13	8.7	6.2	17.8	9.8	8.9	15	15	15

P2RX7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2229 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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P2RX7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2229 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments