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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FBLN5 DANCE
Sequence Length (AA) Molecular Weight (Da)
448 50180
Protein Name
Fibulin-5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGIKRILTV TILALCLPSP GNAQAQCTNG FDLDRQSGQC LDIDECRTIP
60 70 80 90 100
EACRGDMMCV NQNGGYLCIP RTNPVYRGPY SNPYSTPYSG PYPAAAPPLS
110 120 130 140 150
APNYPTISRP LICRFGYQMD ESNQCVDVDE CATDSHQCNP TQICINTEGG
160 170 180 190 200
YTCSCTDGYW LLEGQCLDID ECRYGYCQQL CANVPGSYSC TCNPGFTLNE
210 220 230 240 250
DGRSCQDVNE CATENPCVQT CVNTYGSFIC RCDPGYELEE DGVHCSDMDE
260 270 280 290 300
CSFSEFLCQH ECVNQPGTYF CSCPPGYILL DDNRSCQDIN ECEHRNHTCN
310 320 330 340 350
LQQTCYNLQG GFKCIDPIRC EEPYLRISDN RCMCPAENPG CRDQPFTILY
360 370 380 390 400
RDMDVVSGRS VPADIFQMQA TTRYPGAYYI FQIKSGNEGR EFYMRQTGPI
410 420 430 440 448
SATLVMTRPI KGPREIQLDL EMITVNTVIN FRGSSVIRLR IYVSQYPF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2212 Collapse assay details

Data source: Panorama

Official Gene Symbol
FBLN5
Peptide Modified Sequence
N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
Modification Type
Deamidated (N), Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
296, 299, 305
Peptide - Site of Modification
1, 4, 10
Peptide Start
296
Peptide End
313
CPTAC ID
CPTAC-2212
Peptide Molecular Mass
2,182.9524
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 32.5 9.8 5.4 29.7 14.3 8 44 17.3 9.7 15 15 15
y8 (1+) 10.1 12.2 8.2 11.8 9.8 11.7 15.5 15.6 14.3 15 15 15
y4 (1+) 20.8 5.9 6.3 21.2 7.5 7.7 29.7 9.5 9.9 15 15 15
sum 16.4 4.2 4.1 15.3 7.1 6.5 22.4 8.2 7.7 15 15 15


Additional Resources and Comments