FBLN5, CPTAC-2212 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FBLN5	DANCE

Sequence Length (AA)	Molecular Weight (Da)
448	50180

Protein Name
Fibulin-5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPGIKRILTV	TILALCLPSP	GNAQAQCTNG	FDLDRQSGQC	LDIDECRTIP
60	70	80	90	100
EACRGDMMCV	NQNGGYLCIP	RTNPVYRGPY	SNPYSTPYSG	PYPAAAPPLS
110	120	130	140	150
APNYPTISRP	LICRFGYQMD	ESNQCVDVDE	CATDSHQCNP	TQICINTEGG
160	170	180	190	200
YTCSCTDGYW	LLEGQCLDID	ECRYGYCQQL	CANVPGSYSC	TCNPGFTLNE
210	220	230	240	250
DGRSCQDVNE	CATENPCVQT	CVNTYGSFIC	RCDPGYELEE	DGVHCSDMDE
260	270	280	290	300
CSFSEFLCQH	ECVNQPGTYF	CSCPPGYILL	DDNRSCQDIN	ECEHRNHTCN
310	320	330	340	350
LQQTCYNLQG	GFKCIDPIRC	EEPYLRISDN	RCMCPAENPG	CRDQPFTILY
360	370	380	390	400
RDMDVVSGRS	VPADIFQMQA	TTRYPGAYYI	FQIKSGNEGR	EFYMRQTGPI
410	420	430	440	448
SATLVMTRPI	KGPREIQLDL	EMITVNTVIN	FRGSSVIRLR	IYVSQYPF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2212 Collapse assay details

Data source: Panorama

Official Gene Symbol: FBLN5
Peptide Modified Sequence: N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
Modification Type: Deamidated (N), Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 296, 299, 305
Peptide - Site of Modification: 1, 4, 10
Peptide Start: 296
Peptide End: 313

CPTAC ID: CPTAC-2212
Peptide Molecular Mass: 2,182.9524
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	32.5	9.8	5.4	29.7	14.3	8	44	17.3	9.7	15	15	15
y8 (1+)	10.1	12.2	8.2	11.8	9.8	11.7	15.5	15.6	14.3	15	15	15
y4 (1+)	20.8	5.9	6.3	21.2	7.5	7.7	29.7	9.5	9.9	15	15	15
sum	16.4	4.2	4.1	15.3	7.1	6.5	22.4	8.2	7.7	15	15	15

FBLN5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2212 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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FBLN5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2212 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments