TLR8, CPTAC-2235 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TLR8	N/A

Sequence Length (AA)	Molecular Weight (Da)
1041	119828

Protein Name
Toll-like receptor 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MENMFLQSSM	LTCIFLLISG	SCELCAEENF	SRSYPCDEKK	QNDSVIAECS
60	70	80	90	100
NRRLQEVPQT	VGKYVTELDL	SDNFITHITN	ESFQGLQNLT	KINLNHNPNV
110	120	130	140	150
QHQNGNPGIQ	SNGLNITDGA	FLNLKNLREL	LLEDNQLPQI	PSGLPESLTE
160	170	180	190	200
LSLIQNNIYN	ITKEGISRLI	NLKNLYLAWN	CYFNKVCEKT	NIEDGVFETL
210	220	230	240	250
TNLELLSLSF	NSLSHVPPKL	PSSLRKLFLS	NTQIKYISEE	DFKGLINLTL
260	270	280	290	300
LDLSGNCPRC	FNAPFPCVPC	DGGASINIDR	FAFQNLTQLR	YLNLSSTSLR
310	320	330	340	350
KINAAWFKNM	PHLKVLDLEF	NYLVGEIASG	AFLTMLPRLE	ILDLSFNYIK
360	370	380	390	400
GSYPQHINIS	RNFSKLLSLR	ALHLRGYVFQ	ELREDDFQPL	MQLPNLSTIN
410	420	430	440	450
LGINFIKQID	FKLFQNFSNL	EIIYLSENRI	SPLVKDTRQS	YANSSSFQRH
460	470	480	490	500
IRKRRSTDFE	FDPHSNFYHF	TRPLIKPQCA	AYGKALDLSL	NSIFFIGPNQ
510	520	530	540	550
FENLPDIACL	NLSANSNAQV	LSGTEFSAIP	HVKYLDLTNN	RLDFDNASAL
560	570	580	590	600
TELSDLEVLD	LSYNSHYFRI	AGVTHHLEFI	QNFTNLKVLN	LSHNNIYTLT
610	620	630	640	650
DKYNLESKSL	VELVFSGNRL	DILWNDDDNR	YISIFKGLKN	LTRLDLSLNR
660	670	680	690	700
LKHIPNEAFL	NLPASLTELH	INDNMLKFFN	WTLLQQFPRL	ELLDLRGNKL
710	720	730	740	750
LFLTDSLSDF	TSSLRTLLLS	HNRISHLPSG	FLSEVSSLKH	LDLSSNLLKT
760	770	780	790	800
INKSALETKT	TTKLSMLELH	GNPFECTCDI	GDFRRWMDEH	LNVKIPRLVD
810	820	830	840	850
VICASPGDQR	GKSIVSLELT	TCVSDVTAVI	LFFFTFFITT	MVMLAALAHH
860	870	880	890	900
LFYWDVWFIY	NVCLAKVKGY	RSLSTSQTFY	DAYISYDTKD	ASVTDWVINE
910	920	930	940	950
LRYHLEESRD	KNVLLCLEER	DWDPGLAIID	NLMQSINQSK	KTVFVLTKKY
960	970	980	990	1000
AKSWNFKTAF	YLALQRLMDE	NMDVIIFILL	EPVLQHSQYL	RLRQRICKSS
1010	1020	1030	1040	1041
ILQWPDNPKA	EGLFWQTLRN	VVLTENDSRY	NNMYVDSIKQ	Y

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2235 Collapse assay details

Data source: Panorama

Official Gene Symbol: TLR8
Peptide Modified Sequence: GSYPQHIN[+1.0]ISR
Modification Type: Deamidated (N)
Protein - Site of Modification: 358
Peptide - Site of Modification: 8
Peptide Start: 351
Peptide End: 361

CPTAC ID: CPTAC-2235
Peptide Molecular Mass: 1,271.6259
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	3.6	1.3	1.6	4.2	1.4	1.5	5.5	1.9	2.2	15	15	15
y6 (2+)	4.3	3.6	3.9	4.9	3.2	4.7	6.5	4.8	6.1	15	15	15
y9 (2+)	3.9	1.6	1.5	5.1	1.3	1.5	6.4	2.1	2.1	15	15	15
sum	3.3	1.2	1.1	4	1.2	1.2	5.2	1.7	1.6	15	15	15

TLR8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2235 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TLR8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2235 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments