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Overview Data source: UniProt

Official Gene Symbol Other Aliases
TLR8 N/A
Sequence Length (AA) Molecular Weight (Da)
1041 119828
Protein Name
Toll-like receptor 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENMFLQSSM LTCIFLLISG SCELCAEENF SRSYPCDEKK QNDSVIAECS
60 70 80 90 100
NRRLQEVPQT VGKYVTELDL SDNFITHITN ESFQGLQNLT KINLNHNPNV
110 120 130 140 150
QHQNGNPGIQ SNGLNITDGA FLNLKNLREL LLEDNQLPQI PSGLPESLTE
160 170 180 190 200
LSLIQNNIYN ITKEGISRLI NLKNLYLAWN CYFNKVCEKT NIEDGVFETL
210 220 230 240 250
TNLELLSLSF NSLSHVPPKL PSSLRKLFLS NTQIKYISEE DFKGLINLTL
260 270 280 290 300
LDLSGNCPRC FNAPFPCVPC DGGASINIDR FAFQNLTQLR YLNLSSTSLR
310 320 330 340 350
KINAAWFKNM PHLKVLDLEF NYLVGEIASG AFLTMLPRLE ILDLSFNYIK
360 370 380 390 400
GSYPQHINIS RNFSKLLSLR ALHLRGYVFQ ELREDDFQPL MQLPNLSTIN
410 420 430 440 450
LGINFIKQID FKLFQNFSNL EIIYLSENRI SPLVKDTRQS YANSSSFQRH
460 470 480 490 500
IRKRRSTDFE FDPHSNFYHF TRPLIKPQCA AYGKALDLSL NSIFFIGPNQ
510 520 530 540 550
FENLPDIACL NLSANSNAQV LSGTEFSAIP HVKYLDLTNN RLDFDNASAL
560 570 580 590 600
TELSDLEVLD LSYNSHYFRI AGVTHHLEFI QNFTNLKVLN LSHNNIYTLT
610 620 630 640 650
DKYNLESKSL VELVFSGNRL DILWNDDDNR YISIFKGLKN LTRLDLSLNR
660 670 680 690 700
LKHIPNEAFL NLPASLTELH INDNMLKFFN WTLLQQFPRL ELLDLRGNKL
710 720 730 740 750
LFLTDSLSDF TSSLRTLLLS HNRISHLPSG FLSEVSSLKH LDLSSNLLKT
760 770 780 790 800
INKSALETKT TTKLSMLELH GNPFECTCDI GDFRRWMDEH LNVKIPRLVD
810 820 830 840 850
VICASPGDQR GKSIVSLELT TCVSDVTAVI LFFFTFFITT MVMLAALAHH
860 870 880 890 900
LFYWDVWFIY NVCLAKVKGY RSLSTSQTFY DAYISYDTKD ASVTDWVINE
910 920 930 940 950
LRYHLEESRD KNVLLCLEER DWDPGLAIID NLMQSINQSK KTVFVLTKKY
960 970 980 990 1000
AKSWNFKTAF YLALQRLMDE NMDVIIFILL EPVLQHSQYL RLRQRICKSS
1010 1020 1030 1040 1041
ILQWPDNPKA EGLFWQTLRN VVLTENDSRY NNMYVDSIKQ Y

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2235 Collapse assay details

Data source: Panorama

Official Gene Symbol
TLR8
Peptide Modified Sequence
GSYPQHIN[+1.0]ISR
Modification Type
Deamidated (N)
Protein - Site of Modification
358
Peptide - Site of Modification
8
Peptide Start
351
Peptide End
361
CPTAC ID
CPTAC-2235
Peptide Molecular Mass
1,271.6259
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 3.6 1.3 1.6 4.2 1.4 1.5 5.5 1.9 2.2 15 15 15
y6 (2+) 4.3 3.6 3.9 4.9 3.2 4.7 6.5 4.8 6.1 15 15 15
y9 (2+) 3.9 1.6 1.5 5.1 1.3 1.5 6.4 2.1 2.1 15 15 15
sum 3.3 1.2 1.1 4 1.2 1.2 5.2 1.7 1.6 15 15 15

Additional Resources and Comments