ACE, CPTAC-2597 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACE	DCP, DCP1

Sequence Length (AA)	Molecular Weight (Da)
1306	149715

Protein Name
Angiotensin-converting enzyme

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAASGRRGP	GLLLPLPLLL	LLPPQPALAL	DPGLQPGNFS	ADEAGAQLFA
60	70	80	90	100
QSYNSSAEQV	LFQSVAASWA	HDTNITAENA	RRQEEAALLS	QEFAEAWGQK
110	120	130	140	150
AKELYEPIWQ	NFTDPQLRRI	IGAVRTLGSA	NLPLAKRQQY	NALLSNMSRI
160	170	180	190	200
YSTAKVCLPN	KTATCWSLDP	DLTNILASSR	SYAMLLFAWE	GWHNAAGIPL
210	220	230	240	250
KPLYEDFTAL	SNEAYKQDGF	TDTGAYWRSW	YNSPTFEDDL	EHLYQQLEPL
260	270	280	290	300
YLNLHAFVRR	ALHRRYGDRY	INLRGPIPAH	LLGDMWAQSW	ENIYDMVVPF
310	320	330	340	350
PDKPNLDVTS	TMLQQGWNAT	HMFRVAEEFF	TSLELSPMPP	EFWEGSMLEK
360	370	380	390	400
PADGREVVCH	ASAWDFYNRK	DFRIKQCTRV	TMDQLSTVHH	EMGHIQYYLQ
410	420	430	440	450
YKDLPVSLRR	GANPGFHEAI	GDVLALSVST	PEHLHKIGLL	DRVTNDTESD
460	470	480	490	500
INYLLKMALE	KIAFLPFGYL	VDQWRWGVFS	GRTPPSRYNF	DWWYLRTKYQ
510	520	530	540	550
GICPPVTRNE	THFDAGAKFH	VPNVTPYIRY	FVSFVLQFQF	HEALCKEAGY
560	570	580	590	600
EGPLHQCDIY	RSTKAGAKLR	KVLQAGSSRP	WQEVLKDMVG	LDALDAQPLL
610	620	630	640	650
KYFQPVTQWL	QEQNQQNGEV	LGWPEYQWHP	PLPDNYPEGI	DLVTDEAEAS
660	670	680	690	700
KFVEEYDRTS	QVVWNEYAEA	NWNYNTNITT	ETSKILLQKN	MQIANHTLKY
710	720	730	740	750
GTQARKFDVN	QLQNTTIKRI	IKKVQDLERA	ALPAQELEEY	NKILLDMETT
760	770	780	790	800
YSVATVCHPN	GSCLQLEPDL	TNVMATSRKY	EDLLWAWEGW	RDKAGRAILQ
810	820	830	840	850
FYPKYVELIN	QAARLNGYVD	AGDSWRSMYE	TPSLEQDLER	LFQELQPLYL
860	870	880	890	900
NLHAYVRRAL	HRHYGAQHIN	LEGPIPAHLL	GNMWAQTWSN	IYDLVVPFPS
910	920	930	940	950
APSMDTTEAM	LKQGWTPRRM	FKEADDFFTS	LGLLPVPPEF	WNKSMLEKPT
960	970	980	990	1000
DGREVVCHAS	AWDFYNGKDF	RIKQCTTVNL	EDLVVAHHEM	GHIQYFMQYK
1010	1020	1030	1040	1050
DLPVALREGA	NPGFHEAIGD	VLALSVSTPK	HLHSLNLLSS	EGGSDEHDIN
1060	1070	1080	1090	1100
FLMKMALDKI	AFIPFSYLVD	QWRWRVFDGS	ITKENYNQEW	WSLRLKYQGL
1110	1120	1130	1140	1150
CPPVPRTQGD	FDPGAKFHIP	SSVPYIRYFV	SFIIQFQFHE	ALCQAAGHTG
1160	1170	1180	1190	1200
PLHKCDIYQS	KEAGQRLATA	MKLGFSRPWP	EAMQLITGQP	NMSASAMLSY
1210	1220	1230	1240	1250
FKPLLDWLRT	ENELHGEKLG	WPQYNWTPNS	ARSEGPLPDS	GRVSFLGLDL
1260	1270	1280	1290	1300
DAQQARVGQW	LLLFLGIALL	VATLGLSQRL	FSIRHRSLHR	HSHGPQFGSE
1306
VELRHS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2597 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACE
Peptide Modified Sequence: ELYEPIWQN[+1.0]FTDPQLR
Modification Type: Deamidated (N)
Protein - Site of Modification: 111
Peptide - Site of Modification: 9
Peptide Start: 103
Peptide End: 118

CPTAC ID: CPTAC-2597
Peptide Molecular Mass: 2,048.9844
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

CPTAC-2597: ACE. ELYEPIWQN[+1.0]FTDPQLR	CPTAC-2598: BST1. N[+1.0]C[+57.0]TAIWEAFK	CPTAC-2599: HYOU1. VIN[+1.0]ETWAWK
CPTAC-2600: ICOSLG. TDNSLLDQALQN[+1.0]DTVFLNM[+16.0]R	CPTAC-2601: IGF2R. TN[+1.0]ITLVC[+57.0]KPGDLESAPVLR	CPTAC-2602: PECAM1. DDVLFYN[+1.0]ISSM[+16.0]K
CPTAC-2603: PLXNB2. SIN[+1.0]VTGQGFSLIQR	CPTAC-2604: TNC. LLETVEYN[+1.0]ISGAER	CPTAC-2605: KAT5. SHDDIVTR
CPTAC-2606: KAT5. GFHIVGYFSK	CPTAC-2607: HDAC1. YGEYFPGTGDLR	CPTAC-2608: TP53BP1. ITDVYYVDGTEVER
CPTAC-2609: TP53BP1. WSSNGYFYSGK	CPTAC-2610: BRCA1. TSVYIELGSDSSEDTVNK	CPTAC-2611: BRCA1. SVESNIEDK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	60.9	14.4	13.3	70.4	15.8	16.5	93.1	21.4	21.2	15	15	15
y5 (1+)	43.9	19.9	14.6	44.6	19.8	16.9	62.6	28.1	22.3	15	15	15
y4 (1+)	26.3	7.4	7.7	24.9	11.4	9.3	36.2	13.6	12.1	15	15	15
sum	25.9	5.7	8.1	23.5	10.4	9.7	35	11.9	12.6	15	15	15

ACE

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2597 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

ACE

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2597 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments