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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ICOSLG

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ICOSLG B7H2, B7RP1, ICOSL, KIAA0653
Sequence Length (AA) Molecular Weight (Da)
302 33349
Protein Name
ICOS ligand
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLGSPGLLF LLFSSLRADT QEKEVRAMVG SDVELSCACP EGSRFDLNDV
60 70 80 90 100
YVYWQTSESK TVVTYHIPQN SSLENVDSRY RNRALMSPAG MLRGDFSLRL
110 120 130 140 150
FNVTPQDEQK FHCLVLSQSL GFQEVLSVEV TLHVAANFSV PVVSAPHSPS
160 170 180 190 200
QDELTFTCTS INGYPRPNVY WINKTDNSLL DQALQNDTVF LNMRGLYDVV
210 220 230 240 250
SVLRIARTPS VNIGCCIENV LLQQNLTVGS QTGNDIGERD KITENPVSTG
260 270 280 290 300
EKNAATWSIL AVLCLLVVVA VAIGWVCRDR CLQHSYAGAW AVSPETELTG
302
HV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5937 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICOSLG
Peptide Sequence
GLYDVVSVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
195
Peptide End
204
CPTAC ID
CPTAC-5937
Peptide Molecular Mass
1,119.6288
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.5 7.3 2.5 15.1 16.7 12.7 17.3 18.2 12.9 13 25 14
y8 (1+) 13.7 6.9 3.3 32.9 26.4 19.3 35.6 27.3 19.6 13 25 14
y7 (1+) 7.3 7.2 4.2 15.7 15.9 12.1 17.3 17.5 12.8 13 25 14
y5 (1+) 7.2 5.9 3.5 13.8 15.4 12.8 15.6 16.5 13.3 13 25 14
y4 (1+) 7.6 5.9 5.3 13.5 16.5 12.8 15.5 17.5 13.9 13 25 14
b4 (1+) 8.8 7.4 3.6 13.3 16.7 13.7 15.9 18.3 14.2 13 25 14
sum 7.9 6.2 3 15.3 16.7 13.1 17.2 17.8 13.4 13 25 14

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-2600 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICOSLG
Peptide Modified Sequence
TDNSLLDQALQN[+1.0]DTVFLNM[+16.0]R
Modification Type
Deamidated (N), Oxidation (M)
Protein - Site of Modification
186, 193
Peptide - Site of Modification
12, 19
Peptide Start
175
Peptide End
194
CPTAC ID
CPTAC-2600
Peptide Molecular Mass
2,324.0954
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 37.8 20.1 20.6 29.9 20.3 23.5 48.2 28.6 31.3 15 15 15
y5 (1+) 18.1 10.4 7.4 20.7 16.3 8.4 27.5 19.3 11.2 15 15 15
y4 (1+) 31.8 13.1 14.3 28.7 14.8 13.9 42.8 19.8 19.9 15 15 15
sum 14.2 9.9 6.2 16.4 15.5 8.2 21.7 18.4 10.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments