PLXNB2, CPTAC-2603 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PLXNB2	KIAA0315

Sequence Length (AA)	Molecular Weight (Da)
1838	205127

Protein Name
Plexin-B2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALQLWALTL	LGLLGAGASL	RPRKLDFFRS	EKELNHLAVD	EASGVVYLGA
60	70	80	90	100
VNALYQLDAK	LQLEQQVATG	PALDNKKCTP	PIEASQCHEA	EMTDNVNQLL
110	120	130	140	150
LLDPPRKRLV	ECGSLFKGIC	ALRALSNISL	RLFYEDGSGE	KSFVASNDEG
160	170	180	190	200
VATVGLVSST	GPGGDRVLFV	GKGNGPHDNG	IIVSTRLLDR	TDSREAFEAY
210	220	230	240	250
TDHATYKAGY	LSTNTQQFVA	AFEDGPYVFF	VFNQQDKHPA	RNRTLLARMC
260	270	280	290	300
REDPNYYSYL	EMDLQCRDPD	IHAAAFGTCL	AASVAAPGSG	RVLYAVFSRD
310	320	330	340	350
SRSSGGPGAG	LCLFPLDKVH	AKMEANRNAC	YTGTREARDI	FYKPFHGDIQ
360	370	380	390	400
CGGHAPGSSK	SFPCGSEHLP	YPLGSRDGLR	GTAVLQRGGL	NLTAVTVAAE
410	420	430	440	450
NNHTVAFLGT	SDGRILKVYL	TPDGTSSEYD	SILVEINKRV	KRDLVLSGDL
460	470	480	490	500
GSLYAMTQDK	VFRLPVQECL	SYPTCTQCRD	SQDPYCGWCV	VEGRCTRKAE
510	520	530	540	550
CPRAEEASHW	LWSRSKSCVA	VTSAQPQNMS	RRAQGEVQLT	VSPLPALSEE
560	570	580	590	600
DELLCLFGES	PPHPARVEGE	AVICNSPSSI	PVTPPGQDHV	AVTIQLLLRR
610	620	630	640	650
GNIFLTSYQY	PFYDCRQAMS	LEENLPCISC	VSNRWTCQWD	LRYHECREAS
660	670	680	690	700
PNPEDGIVRA	HMEDSCPQFL	GPSPLVIPMN	HETDVNFQGK	NLDTVKGSSL
710	720	730	740	750
HVGSDLLKFM	EPVTMQESGT	FAFRTPKLSH	DANETLPLHL	YVKSYGKNID
760	770	780	790	800
SKLHVTLYNC	SFGRSDCSLC	RAANPDYRCA	WCGGQSRCVY	EALCNTTSEC
810	820	830	840	850
PPPVITRIQP	ETGPLGGGIR	ITILGSNLGV	QAGDIQRISV	AGRNCSFQPE
860	870	880	890	900
RYSVSTRIVC	VIEAAETPFT	GGVEVDVFGK	LGRSPPNVQF	TFQQPKPLSV
910	920	930	940	950
EPQQGPQAGG	TTLTIHGTHL	DTGSQEDVRV	TLNGVPCKVT	KFGAQLQCVT
960	970	980	990	1000
GPQATRGQML	LEVSYGGSPV	PNPGIFFTYR	ENPVLRAFEP	LRSFASGGRS
1010	1020	1030	1040	1050
INVTGQGFSL	IQRFAMVVIA	EPLQSWQPPR	EAESLQPMTV	VGTDYVFHND
1060	1070	1080	1090	1100
TKVVFLSPAV	PEEPEAYNLT	VLIEMDGHRA	LLRTEAGAFE	YVPDPTFENF
1110	1120	1130	1140	1150
TGGVKKQVNK	LIHARGTNLN	KAMTLQEAEA	FVGAERCTMK	TLTETDLYCE
1160	1170	1180	1190	1200
PPEVQPPPKR	RQKRDTTHNL	PEFIVKFGSR	EWVLGRVEYD	TRVSDVPLSL
1210	1220	1230	1240	1250
ILPLVIVPMV	VVIAVSVYCY	WRKSQQAERE	YEKIKSQLEG	LEESVRDRCK
1260	1270	1280	1290	1300
KEFTDLMIEM	EDQTNDVHEA	GIPVLDYKTY	TDRVFFLPSK	DGDKDVMITG
1310	1320	1330	1340	1350
KLDIPEPRRP	VVEQALYQFS	NLLNSKSFLI	NFIHTLENQR	EFSARAKVYF
1360	1370	1380	1390	1400
ASLLTVALHG	KLEYYTDIMH	TLFLELLEQY	VVAKNPKLML	RRSETVVERM
1410	1420	1430	1440	1450
LSNWMSICLY	QYLKDSAGEP	LYKLFKAIKH	QVEKGPVDAV	QKKAKYTLND
1460	1470	1480	1490	1500
TGLLGDDVEY	APLTVSVIVQ	DEGVDAIPVK	VLNCDTISQV	KEKIIDQVYR
1510	1520	1530	1540	1550
GQPCSCWPRP	DSVVLEWRPG	STAQILSDLD	LTSQREGRWK	RVNTLMHYNV
1560	1570	1580	1590	1600
RDGATLILSK	VGVSQQPEDS	QQDLPGERHA	LLEEENRVWH	LVRPTDEVDE
1610	1620	1630	1640	1650
GKSKRGSVKE	KERTKAITEI	YLTRLLSVKG	TLQQFVDNFF	QSVLAPGHAV
1660	1670	1680	1690	1700
PPAVKYFFDF	LDEQAEKHNI	QDEDTIHIWK	TNSLPLRFWV	NILKNPHFIF
1710	1720	1730	1740	1750
DVHVHEVVDA	SLSVIAQTFM	DACTRTEHKL	SRDSPSNKLL	YAKEISTYKK
1760	1770	1780	1790	1800
MVEDYYKGIR	QMVQVSDQDM	NTHLAEISRA	HTDSLNTLVA	LHQLYQYTQK
1810	1820	1830	1838
YYDEIINALE	EDPAAQKMQL	AFRLQQIAAA	LENKVTDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1504 Collapse assay details

Data source: Panorama

Official Gene Symbol: PLXNB2
Peptide Modified Sequence: LSHDAN[+1.0]ETLPLHLYVK
Modification Type: Deamidated (N)
Protein - Site of Modification: 733
Peptide - Site of Modification: 6
Peptide Start: 728
Peptide End: 743

CPTAC ID: CPTAC-1504
Peptide Molecular Mass: 1,849.9574
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

CPTAC-2597: ACE. ELYEPIWQN[+1.0]FTDPQLR	CPTAC-2598: BST1. N[+1.0]C[+57.0]TAIWEAFK	CPTAC-2599: HYOU1. VIN[+1.0]ETWAWK
CPTAC-2600: ICOSLG. TDNSLLDQALQN[+1.0]DTVFLNM[+16.0]R	CPTAC-2601: IGF2R. TN[+1.0]ITLVC[+57.0]KPGDLESAPVLR	CPTAC-2602: PECAM1. DDVLFYN[+1.0]ISSM[+16.0]K
CPTAC-2603: PLXNB2. SIN[+1.0]VTGQGFSLIQR	CPTAC-2604: TNC. LLETVEYN[+1.0]ISGAER	CPTAC-2605: KAT5. SHDDIVTR
CPTAC-2606: KAT5. GFHIVGYFSK	CPTAC-2607: HDAC1. YGEYFPGTGDLR	CPTAC-2608: TP53BP1. ITDVYYVDGTEVER
CPTAC-2609: TP53BP1. WSSNGYFYSGK	CPTAC-2610: BRCA1. TSVYIELGSDSSEDTVNK	CPTAC-2611: BRCA1. SVESNIEDK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	14.8	4	7.1	23.6	5.3	6.9	27.9	6.6	9.9	15	15	15
y7 (2+)	28.4	5.4	5.1	67.2	7.1	6.2	73	8.9	8	15	15	15
y7 (1+)	9.6	8.4	4.3	11.3	9.9	4.6	14.8	13	6.3	15	15	15
sum	16.1	4.7	3.1	35.1	6.1	3.2	38.6	7.7	4.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2603 Collapse assay details

Data source: Panorama

Official Gene Symbol: PLXNB2
Peptide Modified Sequence: SIN[+1.0]VTGQGFSLIQR
Modification Type: Deamidated (N)
Protein - Site of Modification: 1002
Peptide - Site of Modification: 3
Peptide Start: 1000
Peptide End: 1013

CPTAC ID: CPTAC-2603
Peptide Molecular Mass: 1,519.7995
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang