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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

IGF2R

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
IGF2R MPRI
Sequence Length (AA) Molecular Weight (Da)
2491 274375
Protein Name
Cation-independent mannose-6-phosphate receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAAAGRSPH LGPAPARRPQ RSLLLLQLLL LVAAPGSTQA QAAPFPELCS
60 70 80 90 100
YTWEAVDTKN NVLYKINICG SVDIVQCGPS SAVCMHDLKT RTYHSVGDSV
110 120 130 140 150
LRSATRSLLE FNTTVSCDQQ GTNHRVQSSI AFLCGKTLGT PEFVTATECV
160 170 180 190 200
HYFEWRTTAA CKKDIFKANK EVPCYVFDEE LRKHDLNPLI KLSGAYLVDD
210 220 230 240 250
SDPDTSLFIN VCRDIDTLRD PGSQLRACPP GTAACLVRGH QAFDVGQPRD
260 270 280 290 300
GLKLVRKDRL VLSYVREEAG KLDFCDGHSP AVTITFVCPS ERREGTIPKL
310 320 330 340 350
TAKSNCRYEI EWITEYACHR DYLESKTCSL SGEQQDVSID LTPLAQSGGS
360 370 380 390 400
SYISDGKEYL FYLNVCGETE IQFCNKKQAA VCQVKKSDTS QVKAAGRYHN
410 420 430 440 450
QTLRYSDGDL TLIYFGGDEC SSGFQRMSVI NFECNKTAGN DGKGTPVFTG
460 470 480 490 500
EVDCTYFFTW DTEYACVKEK EDLLCGATDG KKRYDLSALV RHAEPEQNWE
510 520 530 540 550
AVDGSQTETE KKHFFINICH RVLQEGKARG CPEDAAVCAV DKNGSKNLGK
560 570 580 590 600
FISSPMKEKG NIQLSYSDGD DCGHGKKIKT NITLVCKPGD LESAPVLRTS
610 620 630 640 650
GEGGCFYEFE WHTAAACVLS KTEGENCTVF DSQAGFSFDL SPLTKKNGAY
660 670 680 690 700
KVETKKYDFY INVCGPVSVS PCQPDSGACQ VAKSDEKTWN LGLSNAKLSY
710 720 730 740 750
YDGMIQLNYR GGTPYNNERH TPRATLITFL CDRDAGVGFP EYQEEDNSTY
760 770 780 790 800
NFRWYTSYAC PEEPLECVVT DPSTLEQYDL SSLAKSEGGL GGNWYAMDNS
810 820 830 840 850
GEHVTWRKYY INVCRPLNPV PGCNRYASAC QMKYEKDQGS FTEVVSISNL
860 870 880 890 900
GMAKTGPVVE DSGSLLLEYV NGSACTTSDG RQTTYTTRIH LVCSRGRLNS
910 920 930 940 950
HPIFSLNWEC VVSFLWNTEA ACPIQTTTDT DQACSIRDPN SGFVFNLNPL
960 970 980 990 1000
NSSQGYNVSG IGKIFMFNVC GTMPVCGTIL GKPASGCEAE TQTEELKNWK
1010 1020 1030 1040 1050
PARPVGIEKS LQLSTEGFIT LTYKGPLSAK GTADAFIVRF VCNDDVYSGP
1060 1070 1080 1090 1100
LKFLHQDIDS GQGIRNTYFE FETALACVPS PVDCQVTDLA GNEYDLTGLS
1110 1120 1130 1140 1150
TVRKPWTAVD TSVDGRKRTF YLSVCNPLPY IPGCQGSAVG SCLVSEGNSW
1160 1170 1180 1190 1200
NLGVVQMSPQ AAANGSLSIM YVNGDKCGNQ RFSTRITFEC AQISGSPAFQ
1210 1220 1230 1240 1250
LQDGCEYVFI WRTVEACPVV RVEGDNCEVK DPRHGNLYDL KPLGLNDTIV
1260 1270 1280 1290 1300
SAGEYTYYFR VCGKLSSDVC PTSDKSKVVS SCQEKREPQG FHKVAGLLTQ
1310 1320 1330 1340 1350
KLTYENGLLK MNFTGGDTCH KVYQRSTAIF FYCDRGTQRP VFLKETSDCS
1360 1370 1380 1390 1400
YLFEWRTQYA CPPFDLTECS FKDGAGNSFD LSSLSRYSDN WEAITGTGDP
1410 1420 1430 1440 1450
EHYLINVCKS LAPQAGTEPC PPEAAACLLG GSKPVNLGRV RDGPQWRDGI
1460 1470 1480 1490 1500
IVLKYVDGDL CPDGIRKKST TIRFTCSESQ VNSRPMFISA VEDCEYTFAW
1510 1520 1530 1540 1550
PTATACPMKS NEHDDCQVTN PSTGHLFDLS SLSGRAGFTA AYSEKGLVYM
1560 1570 1580 1590 1600
SICGENENCP PGVGACFGQT RISVGKANKR LRYVDQVLQL VYKDGSPCPS
1610 1620 1630 1640 1650
KSGLSYKSVI SFVCRPEARP TNRPMLISLD KQTCTLFFSW HTPLACEQAT
1660 1670 1680 1690 1700
ECSVRNGSSI VDLSPLIHRT GGYEAYDESE DDASDTNPDF YINICQPLNP
1710 1720 1730 1740 1750
MHGVPCPAGA AVCKVPIDGP PIDIGRVAGP PILNPIANEI YLNFESSTPC
1760 1770 1780 1790 1800
LADKHFNYTS LIAFHCKRGV SMGTPKLLRT SECDFVFEWE TPVVCPDEVR
1810 1820 1830 1840 1850
MDGCTLTDEQ LLYSFNLSSL STSTFKVTRD SRTYSVGVCT FAVGPEQGGC
1860 1870 1880 1890 1900
KDGGVCLLSG TKGASFGRLQ SMKLDYRHQD EAVVLSYVNG DRCPPETDDG
1910 1920 1930 1940 1950
VPCVFPFIFN GKSYEECIIE SRAKLWCSTT ADYDRDHEWG FCRHSNSYRT
1960 1970 1980 1990 2000
SSIIFKCDED EDIGRPQVFS EVRGCDVTFE WKTKVVCPPK KLECKFVQKH
2010 2020 2030 2040 2050
KTYDLRLLSS LTGSWSLVHN GVSYYINLCQ KIYKGPLGCS ERASICRRTT
2060 2070 2080 2090 2100
TGDVQVLGLV HTQKLGVIGD KVVVTYSKGY PCGGNKTASS VIELTCTKTV
2110 2120 2130 2140 2150
GRPAFKRFDI DSCTYYFSWD SRAACAVKPQ EVQMVNGTIT NPINGKSFSL
2160 2170 2180 2190 2200
GDIYFKLFRA SGDMRTNGDN YLYEIQLSSI TSSRNPACSG ANICQVKPND
2210 2220 2230 2240 2250
QHFSRKVGTS DKTKYYLQDG DLDVVFASSS KCGKDKTKSV SSTIFFHCDP
2260 2270 2280 2290 2300
LVEDGIPEFS HETADCQYLF SWYTSAVCPL GVGFDSENPG DDGQMHKGLS
2310 2320 2330 2340 2350
ERSQAVGAVL SLLLVALTCC LLALLLYKKE RRETVISKLT TCCRRSSNVS
2360 2370 2380 2390 2400
YKYSKVNKEE ETDENETEWL MEEIQLPPPR QGKEGQENGH ITTKSVKALS
2410 2420 2430 2440 2450
SLHGDDQDSE DEVLTIPEVK VHSGRGAGAE SSHPVRNAQS NALQEREDDR
2460 2470 2480 2490 2491
VGLVRGEKAR KGKSSSAQQK TVSSTKLVSF HDDSDEDLLH I

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2601 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGF2R
Peptide Modified Sequence
TN[+1.0]ITLVC[+57.0]KPGDLESAPVLR
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
581, 586
Peptide - Site of Modification
2, 7
Peptide Start
580
Peptide End
598
CPTAC ID
CPTAC-2601
Peptide Molecular Mass
2,083.0983
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 20.5 4.4 8.5 18.8 6.9 9.2 27.8 8.2 12.5 15 15 15
y6 (1+) 21.9 12.6 11.3 22.6 10.8 12.6 31.5 16.6 16.9 15 15 15
y4 (1+) 25.6 10 9.2 23.8 11 9.1 35 14.9 12.9 15 15 15
y14 (2+) 23.7 16.5 14.1 32.5 17.1 12 40.2 23.8 18.5 15 15 15
sum 8.1 5.6 3.4 10 5.7 3.9 12.9 8 5.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-2642 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGF2R
Peptide Sequence
GHQAFDVGQPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
239
Peptide End
249
CPTAC ID
non-CPTAC-2642
Peptide Molecular Mass
1,210.5843
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 17.6 7 5.6 28.1 12 7.3 33.2 13.9 9.2 15 15 15
y2 (1+) 16.6 8.6 6.4 20.4 13.6 12.1 26.3 16.1 13.7 15 15 15
y4 (1+) 6.1 6.3 7.2 8.8 10.1 10.1 10.7 11.9 12.4 15 15 15
b3 (1+) 17.6 11 7.7 26.7 16.9 11.7 32 20.2 14 15 15 15
b6 (1+) 15.3 6.4 7.9 15.8 10.4 10.5 22 12.2 13.1 15 15 15
sum 5.8 4.3 6 12.4 10 8.2 13.7 10.9 10.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments