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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

HYOU1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HYOU1 GRP170, ORP150
Sequence Length (AA) Molecular Weight (Da)
999 111335
Protein Name
Hypoxia up-regulated protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MADKVRRQRP RRRVCWALVA VLLADLLALS DTLAVMSVDL GSESMKVAIV
60 70 80 90 100
KPGVPMEIVL NKESRRKTPV IVTLKENERF FGDSAASMAI KNPKATLRYF
110 120 130 140 150
QHLLGKQADN PHVALYQARF PEHELTFDPQ RQTVHFQISS QLQFSPEEVL
160 170 180 190 200
GMVLNYSRSL AEDFAEQPIK DAVITVPVFF NQAERRAVLQ AARMAGLKVL
210 220 230 240 250
QLINDNTATA LSYGVFRRKD INTTAQNIMF YDMGSGSTVC TIVTYQMVKT
260 270 280 290 300
KEAGMQPQLQ IRGVGFDRTL GGLEMELRLR ERLAGLFNEQ RKGQRAKDVR
310 320 330 340 350
ENPRAMAKLL REANRLKTVL SANADHMAQI EGLMDDVDFK AKVTRVEFEE
360 370 380 390 400
LCADLFERVP GPVQQALQSA EMSLDEIEQV ILVGGATRVP RVQEVLLKAV
410 420 430 440 450
GKEELGKNIN ADEAAAMGAV YQAAALSKAF KVKPFVVRDA VVYPILVEFT
460 470 480 490 500
REVEEEPGIH SLKHNKRVLF SRMGPYPQRK VITFNRYSHD FNFHINYGDL
510 520 530 540 550
GFLGPEDLRV FGSQNLTTVK LKGVGDSFKK YPDYESKGIK AHFNLDESGV
560 570 580 590 600
LSLDRVESVF ETLVEDSAEE ESTLTKLGNT ISSLFGGGTT PDAKENGTDT
610 620 630 640 650
VQEEEESPAE GSKDEPGEQV ELKEEAEAPV EDGSQPPPPE PKGDATPEGE
660 670 680 690 700
KATEKENGDK SEAQKPSEKA EAGPEGVAPA PEGEKKQKPA RKRRMVEEIG
710 720 730 740 750
VELVVLDLPD LPEDKLAQSV QKLQDLTLRD LEKQEREKAA NSLEAFIFET
760 770 780 790 800
QDKLYQPEYQ EVSTEEQREE ISGKLSAAST WLEDEGVGAT TVMLKEKLAE
810 820 830 840 850
LRKLCQGLFF RVEERKKWPE RLSALDNLLN HSSMFLKGAR LIPEMDQIFT
860 870 880 890 900
EVEMTTLEKV INETWAWKNA TLAEQAKLPA TEKPVLLSKD IEAKMMALDR
910 920 930 940 950
EVQYLLNKAK FTKPRPRPKD KNGTRAEPPL NASASDQGEK VIPPAGQTED
960 970 980 990 999
AEPISEPEKV ETGSEPGDTE PLELGGPGAE PEQKEQSTGQ KRPLKNDEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2220 Collapse assay details

Data source: Panorama

Official Gene Symbol
HYOU1
Peptide Modified Sequence
VFGSQN[+1.0]LTTVK
Modification Type
Deamidated (N)
Protein - Site of Modification
515
Peptide - Site of Modification
6
Peptide Start
510
Peptide End
520
CPTAC ID
CPTAC-2220
Peptide Molecular Mass
1,193.6292
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 9 5.3 4.5 10.2 5.4 4.2 13.6 7.6 6.2 15 15 15
y6 (1+) 16.2 13.6 10.9 12.8 11.9 12.4 20.6 18.1 16.5 15 15 15
y9 (1+) 6.6 2.8 4.9 6.9 3.8 4.4 9.5 4.7 6.6 15 15 15
sum 7.9 2.5 3.9 8 3 3.3 11.2 3.9 5.1 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-2599 Collapse assay details

Data source: Panorama

Official Gene Symbol
HYOU1
Peptide Modified Sequence
VIN[+1.0]ETWAWK
Modification Type
Deamidated (N)
Protein - Site of Modification
862
Peptide - Site of Modification
3
Peptide Start
860
Peptide End
868
CPTAC ID
CPTAC-2599
Peptide Molecular Mass
1,146.5710
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 62.5 18.8 18.3 60 23.2 20.3 86.6 29.9 27.3 15 15 15
y8 (1+) 81.7 19.7 18.5 91 22.5 21.3 122.3 29.9 28.2 15 15 15
y7 (1+) 19 6.7 4.4 16.1 7.1 5.1 24.9 9.8 6.7 15 15 15
y6 (1+) 67.9 27.2 13.6 84.3 22 15.6 108.2 35 20.7 15 15 15
sum 19 6.4 3.6 14.1 6.9 4.8 23.7 9.4 6 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3714 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hyou1
Peptide Sequence
LYQPEYQEVSTEEQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
754
Peptide End
768
CPTAC ID
non-CPTAC-3714
Peptide Molecular Mass
1,897.8694
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12 10.8 8.1 12.1 11.1 9.3 17 15.5 12.3 15 15 15
y6 (1+) 8.8 6.2 5.9 16.6 7.4 7.3 18.8 9.7 9.4 15 15 15
b10 (2+) 32.3 22.9 24.4 35.3 26.1 21.3 47.8 34.7 32.4 15 15 15
b9 (2+) 18.5 16.4 12.8 23.4 19.2 13.7 29.8 25.3 18.7 15 15 15
b5 (1+) 22.8 14.8 12.6 30.2 16.2 10.4 37.8 21.9 16.3 15 15 15
sum 6.7 3.8 5.9 13.3 6.6 7 14.9 7.6 9.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments