KAT5, CPTAC-2605 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KAT5	HTATIP, TIP60

Sequence Length (AA)	Molecular Weight (Da)
513	58582

Protein Name
Histone acetyltransferase KAT5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAEVGEIIEG	CRLPVLRRNQ	DNEDEWPLAE	ILSVKDISGR	KLFYVHYIDF
60	70	80	90	100
NKRLDEWVTH	ERLDLKKIQF	PKKEAKTPTK	NGLPGSRPGS	PEREVPASAQ
110	120	130	140	150
ASGKTLPIPV	QITLRFNLPK	EREAIPGGEP	DQPLSSSSCL	QPNHRSTKRK
160	170	180	190	200
VEVVSPATPV	PSETAPASVF	PQNGAARRAV	AAQPGRKRKS	NCLGTDEDSQ
210	220	230	240	250
DSSDGIPSAP	RMTGSLVSDR	SHDDIVTRMK	NIECIELGRH	RLKPWYFSPY
260	270	280	290	300
PQELTTLPVL	YLCEFCLKYG	RSLKCLQRHL	TKCDLRHPPG	NEIYRKGTIS
310	320	330	340	350
FFEIDGRKNK	SYSQNLCLLA	KCFLDHKTLY	YDTDPFLFYV	MTEYDCKGFH
360	370	380	390	400
IVGYFSKEKE	STEDYNVACI	LTLPPYQRRG	YGKLLIEFSY	ELSKVEGKTG
410	420	430	440	450
TPEKPLSDLG	LLSYRSYWSQ	TILEILMGLK	SESGERPQIT	INEISEITSI
460	470	480	490	500
KKEDVISTLQ	YLNLINYYKG	QYILTLSEDI	VDGHERAMLK	RLLRIDSKCL
510	513
HFTPKDWSKR	GKW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-2606 Collapse assay details

Data source: Panorama

Official Gene Symbol: KAT5
Peptide Sequence: GFHIVGYFSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 348
Peptide End: 357

CPTAC ID: CPTAC-2606
Peptide Molecular Mass: 1,153.5920
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

CPTAC-2597: ACE. ELYEPIWQN[+1.0]FTDPQLR	CPTAC-2598: BST1. N[+1.0]C[+57.0]TAIWEAFK	CPTAC-2599: HYOU1. VIN[+1.0]ETWAWK
CPTAC-2600: ICOSLG. TDNSLLDQALQN[+1.0]DTVFLNM[+16.0]R	CPTAC-2601: IGF2R. TN[+1.0]ITLVC[+57.0]KPGDLESAPVLR	CPTAC-2602: PECAM1. DDVLFYN[+1.0]ISSM[+16.0]K
CPTAC-2603: PLXNB2. SIN[+1.0]VTGQGFSLIQR	CPTAC-2604: TNC. LLETVEYN[+1.0]ISGAER	CPTAC-2605: KAT5. SHDDIVTR
CPTAC-2606: KAT5. GFHIVGYFSK	CPTAC-2607: HDAC1. YGEYFPGTGDLR	CPTAC-2608: TP53BP1. ITDVYYVDGTEVER
CPTAC-2609: TP53BP1. WSSNGYFYSGK	CPTAC-2610: BRCA1. TSVYIELGSDSSEDTVNK	CPTAC-2611: BRCA1. SVESNIEDK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	7.3	7.2	4.8	7.4	12.3	6.1	10.4	14.3	7.8	15	15	15
b4 (1+)	19.1	8.5	6.8	17.5	13.4	7.5	25.9	15.9	10.1	15	15	15
b6 (1+)	9.7	7.7	5.5	9.3	12.7	6.4	13.4	14.9	8.4	15	15	15
sum	5	7	4.7	4.5	12.5	5.8	6.7	14.3	7.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2605 Collapse assay details

Data source: Panorama

Official Gene Symbol: KAT5
Peptide Sequence: SHDDIVTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 221
Peptide End: 228

CPTAC ID: CPTAC-2605
Peptide Molecular Mass: 941.4567
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang