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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PECAM1 N/A
Sequence Length (AA) Molecular Weight (Da)
738 82536
Protein Name
Platelet endothelial cell adhesion molecule
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPRWAQGAT MWLGVLLTLL LCSSLEGQEN SFTINSVDMK SLPDWTVQNG
60 70 80 90 100
KNLTLQCFAD VSTTSHVKPQ HQMLFYKDDV LFYNISSMKS TESYFIPEVR
110 120 130 140 150
IYDSGTYKCT VIVNNKEKTT AEYQLLVEGV PSPRVTLDKK EAIQGGIVRV
160 170 180 190 200
NCSVPEEKAP IHFTIEKLEL NEKMVKLKRE KNSRDQNFVI LEFPVEEQDR
210 220 230 240 250
VLSFRCQARI ISGIHMQTSE STKSELVTVT ESFSTPKFHI SPTGMIMEGA
260 270 280 290 300
QLHIKCTIQV THLAQEFPEI IIQKDKAIVA HNRHGNKAVY SVMAMVEHSG
310 320 330 340 350
NYTCKVESSR ISKVSSIVVN ITELFSKPEL ESSFTHLDQG ERLNLSCSIP
360 370 380 390 400
GAPPANFTIQ KEDTIVSQTQ DFTKIASKSD SGTYICTAGI DKVVKKSNTV
410 420 430 440 450
QIVVCEMLSQ PRISYDAQFE VIKGQTIEVR CESISGTLPI SYQLLKTSKV
460 470 480 490 500
LENSTKNSND PAVFKDNPTE DVEYQCVADN CHSHAKMLSE VLRVKVIAPV
510 520 530 540 550
DEVQISILSS KVVESGEDIV LQCAVNEGSG PITYKFYREK EGKPFYQMTS
560 570 580 590 600
NATQAFWTKQ KASKEQEGEY YCTAFNRANH ASSVPRSKIL TVRVILAPWK
610 620 630 640 650
KGLIAVVIIG VIIALLIIAA KCYFLRKAKA KQMPVEMSRP AVPLLNSNNE
660 670 680 690 700
KMSDPNMEAN SHYGHNDDVR NHAMKPINDN KEPLNSDVQY TEVQVSSAES
710 720 730 738
HKDLGKKDTE TVYSEVRKAV PDAVESRYSR TEGSLDGT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2602 Collapse assay details

Data source: Panorama

Official Gene Symbol
PECAM1
Peptide Modified Sequence
DDVLFYN[+1.0]ISSM[+16.0]K
Modification Type
Deamidated (N), Oxidation (M)
Protein - Site of Modification
84, 88
Peptide - Site of Modification
7, 11
Peptide Start
78
Peptide End
89
CPTAC ID
CPTAC-2602
Peptide Molecular Mass
1,447.6541
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.2 18 13.6 30 16.3 16.9 38.5 24.3 21.7 15 15 15
y4 (1+) 15.2 9.4 9.9 16.9 16.1 10 22.7 18.6 14.1 15 15 15
b4 (1+) 38.4 14.3 10.4 32.1 17.7 9.9 50 22.8 14.4 15 15 15
sum 13.7 7.9 7 15.4 14.8 7.3 20.6 16.8 10.1 15 15 15

Additional Resources and Comments