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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BST1 N/A
Sequence Length (AA) Molecular Weight (Da)
318 35724
Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAQGCAASR LLQLLLQLLL LLLLLAAGGA RARWRGEGTS AHLRDIFLGR
60 70 80 90 100
CAEYRALLSP EQRNKNCTAI WEAFKVALDK DPCSVLPSDY DLFINLSRHS
110 120 130 140 150
IPRDKSLFWE NSHLLVNSFA DNTRRFMPLS DVLYGRVADF LSWCRQKNDS
160 170 180 190 200
GLDYQSCPTS EDCENNPVDS FWKRASIQYS KDSSGVIHVM LNGSEPTGAY
210 220 230 240 250
PIKGFFADYE IPNLQKEKIT RIEIWVMHEI GGPNVESCGE GSMKVLEKRL
260 270 280 290 300
KDMGFQYSCI NDYRPVKLLQ CVDHSTHPDC ALKSAAAATQ RKAPSLYTEQ
310 318
RAGLIIPLFL VLASRTQL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2598 Collapse assay details

Data source: Panorama

Official Gene Symbol
BST1
Peptide Modified Sequence
N[+1.0]C[+57.0]TAIWEAFK
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
66, 67
Peptide - Site of Modification
1, 2
Peptide Start
66
Peptide End
75
CPTAC ID
CPTAC-2598
Peptide Molecular Mass
1,239.5594
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 21.6 5.7 4.5 21.7 5.5 4.5 30.6 7.9 6.4 15 15 15
y5 (1+) 27.9 7.9 6 27.3 7.2 7.4 39 10.7 9.5 15 15 15
y6 (1+) 29.4 7.3 13.2 27 9.8 13.6 39.9 12.2 19 15 15 15
y7 (1+) 21.2 9.6 8.2 14.9 9.4 9 25.9 13.4 12.2 15 15 15
sum 15.6 4.3 3.1 14.8 4.1 4.4 21.5 5.9 5.4 15 15 15


Additional Resources and Comments