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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLXNB2 KIAA0315
Sequence Length (AA) Molecular Weight (Da)
1838 205127
Protein Name
Plexin-B2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALQLWALTL LGLLGAGASL RPRKLDFFRS EKELNHLAVD EASGVVYLGA
60 70 80 90 100
VNALYQLDAK LQLEQQVATG PALDNKKCTP PIEASQCHEA EMTDNVNQLL
110 120 130 140 150
LLDPPRKRLV ECGSLFKGIC ALRALSNISL RLFYEDGSGE KSFVASNDEG
160 170 180 190 200
VATVGLVSST GPGGDRVLFV GKGNGPHDNG IIVSTRLLDR TDSREAFEAY
210 220 230 240 250
TDHATYKAGY LSTNTQQFVA AFEDGPYVFF VFNQQDKHPA RNRTLLARMC
260 270 280 290 300
REDPNYYSYL EMDLQCRDPD IHAAAFGTCL AASVAAPGSG RVLYAVFSRD
310 320 330 340 350
SRSSGGPGAG LCLFPLDKVH AKMEANRNAC YTGTREARDI FYKPFHGDIQ
360 370 380 390 400
CGGHAPGSSK SFPCGSEHLP YPLGSRDGLR GTAVLQRGGL NLTAVTVAAE
410 420 430 440 450
NNHTVAFLGT SDGRILKVYL TPDGTSSEYD SILVEINKRV KRDLVLSGDL
460 470 480 490 500
GSLYAMTQDK VFRLPVQECL SYPTCTQCRD SQDPYCGWCV VEGRCTRKAE
510 520 530 540 550
CPRAEEASHW LWSRSKSCVA VTSAQPQNMS RRAQGEVQLT VSPLPALSEE
560 570 580 590 600
DELLCLFGES PPHPARVEGE AVICNSPSSI PVTPPGQDHV AVTIQLLLRR
610 620 630 640 650
GNIFLTSYQY PFYDCRQAMS LEENLPCISC VSNRWTCQWD LRYHECREAS
660 670 680 690 700
PNPEDGIVRA HMEDSCPQFL GPSPLVIPMN HETDVNFQGK NLDTVKGSSL
710 720 730 740 750
HVGSDLLKFM EPVTMQESGT FAFRTPKLSH DANETLPLHL YVKSYGKNID
760 770 780 790 800
SKLHVTLYNC SFGRSDCSLC RAANPDYRCA WCGGQSRCVY EALCNTTSEC
810 820 830 840 850
PPPVITRIQP ETGPLGGGIR ITILGSNLGV QAGDIQRISV AGRNCSFQPE
860 870 880 890 900
RYSVSTRIVC VIEAAETPFT GGVEVDVFGK LGRSPPNVQF TFQQPKPLSV
910 920 930 940 950
EPQQGPQAGG TTLTIHGTHL DTGSQEDVRV TLNGVPCKVT KFGAQLQCVT
960 970 980 990 1000
GPQATRGQML LEVSYGGSPV PNPGIFFTYR ENPVLRAFEP LRSFASGGRS
1010 1020 1030 1040 1050
INVTGQGFSL IQRFAMVVIA EPLQSWQPPR EAESLQPMTV VGTDYVFHND
1060 1070 1080 1090 1100
TKVVFLSPAV PEEPEAYNLT VLIEMDGHRA LLRTEAGAFE YVPDPTFENF
1110 1120 1130 1140 1150
TGGVKKQVNK LIHARGTNLN KAMTLQEAEA FVGAERCTMK TLTETDLYCE
1160 1170 1180 1190 1200
PPEVQPPPKR RQKRDTTHNL PEFIVKFGSR EWVLGRVEYD TRVSDVPLSL
1210 1220 1230 1240 1250
ILPLVIVPMV VVIAVSVYCY WRKSQQAERE YEKIKSQLEG LEESVRDRCK
1260 1270 1280 1290 1300
KEFTDLMIEM EDQTNDVHEA GIPVLDYKTY TDRVFFLPSK DGDKDVMITG
1310 1320 1330 1340 1350
KLDIPEPRRP VVEQALYQFS NLLNSKSFLI NFIHTLENQR EFSARAKVYF
1360 1370 1380 1390 1400
ASLLTVALHG KLEYYTDIMH TLFLELLEQY VVAKNPKLML RRSETVVERM
1410 1420 1430 1440 1450
LSNWMSICLY QYLKDSAGEP LYKLFKAIKH QVEKGPVDAV QKKAKYTLND
1460 1470 1480 1490 1500
TGLLGDDVEY APLTVSVIVQ DEGVDAIPVK VLNCDTISQV KEKIIDQVYR
1510 1520 1530 1540 1550
GQPCSCWPRP DSVVLEWRPG STAQILSDLD LTSQREGRWK RVNTLMHYNV
1560 1570 1580 1590 1600
RDGATLILSK VGVSQQPEDS QQDLPGERHA LLEEENRVWH LVRPTDEVDE
1610 1620 1630 1640 1650
GKSKRGSVKE KERTKAITEI YLTRLLSVKG TLQQFVDNFF QSVLAPGHAV
1660 1670 1680 1690 1700
PPAVKYFFDF LDEQAEKHNI QDEDTIHIWK TNSLPLRFWV NILKNPHFIF
1710 1720 1730 1740 1750
DVHVHEVVDA SLSVIAQTFM DACTRTEHKL SRDSPSNKLL YAKEISTYKK
1760 1770 1780 1790 1800
MVEDYYKGIR QMVQVSDQDM NTHLAEISRA HTDSLNTLVA LHQLYQYTQK
1810 1820 1830 1838
YYDEIINALE EDPAAQKMQL AFRLQQIAAA LENKVTDL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1504 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLXNB2
Peptide Modified Sequence
LSHDAN[+1.0]ETLPLHLYVK
Modification Type
Deamidated (N)
Protein - Site of Modification
733
Peptide - Site of Modification
6
Peptide Start
728
Peptide End
743
CPTAC ID
CPTAC-1504
Peptide Molecular Mass
1,849.9574
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 14.8 4 7.1 23.6 5.3 6.9 27.9 6.6 9.9 15 15 15
y7 (2+) 28.4 5.4 5.1 67.2 7.1 6.2 73 8.9 8 15 15 15
y7 (1+) 9.6 8.4 4.3 11.3 9.9 4.6 14.8 13 6.3 15 15 15
sum 16.1 4.7 3.1 35.1 6.1 3.2 38.6 7.7 4.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2603 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLXNB2
Peptide Modified Sequence
SIN[+1.0]VTGQGFSLIQR
Modification Type
Deamidated (N)
Protein - Site of Modification
1002
Peptide - Site of Modification
3
Peptide Start
1000
Peptide End
1013
CPTAC ID
CPTAC-2603
Peptide Molecular Mass
1,519.7995
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 84.7 25.3 20.1 146.4 25.9 20 169.1 36.2 28.4 15 15 15
y5 (1+) 69.4 13.7 11.4 110.1 14.5 13.2 130.1 19.9 17.4 15 15 15
y4 (1+) 101.9 15.8 18.1 108.9 21.9 16.4 149.1 27 24.4 15 15 15
sum 68.7 11 11.8 78.1 12 13 104 16.3 17.6 15 15 15


Additional Resources and Comments