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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MET

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MET N/A
Sequence Length (AA) Molecular Weight (Da)
1390 155541
Protein Name
Hepatocyte growth factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKAPAVLAPG ILVLLFTLVQ RSNGECKEAL AKSEMNVNMK YQLPNFTAET
60 70 80 90 100
PIQNVILHEH HIFLGATNYI YVLNEEDLQK VAEYKTGPVL EHPDCFPCQD
110 120 130 140 150
CSSKANLSGG VWKDNINMAL VVDTYYDDQL ISCGSVNRGT CQRHVFPHNH
160 170 180 190 200
TADIQSEVHC IFSPQIEEPS QCPDCVVSAL GAKVLSSVKD RFINFFVGNT
210 220 230 240 250
INSSYFPDHP LHSISVRRLK ETKDGFMFLT DQSYIDVLPE FRDSYPIKYV
260 270 280 290 300
HAFESNNFIY FLTVQRETLD AQTFHTRIIR FCSINSGLHS YMEMPLECIL
310 320 330 340 350
TEKRKKRSTK KEVFNILQAA YVSKPGAQLA RQIGASLNDD ILFGVFAQSK
360 370 380 390 400
PDSAEPMDRS AMCAFPIKYV NDFFNKIVNK NNVRCLQHFY GPNHEHCFNR
410 420 430 440 450
TLLRNSSGCE ARRDEYRTEF TTALQRVDLF MGQFSEVLLT SISTFIKGDL
460 470 480 490 500
TIANLGTSEG RFMQVVVSRS GPSTPHVNFL LDSHPVSPEV IVEHTLNQNG
510 520 530 540 550
YTLVITGKKI TKIPLNGLGC RHFQSCSQCL SAPPFVQCGW CHDKCVRSEE
560 570 580 590 600
CLSGTWTQQI CLPAIYKVFP NSAPLEGGTR LTICGWDFGF RRNNKFDLKK
610 620 630 640 650
TRVLLGNESC TLTLSESTMN TLKCTVGPAM NKHFNMSIII SNGHGTTQYS
660 670 680 690 700
TFSYVDPVIT SISPKYGPMA GGTLLTLTGN YLNSGNSRHI SIGGKTCTLK
710 720 730 740 750
SVSNSILECY TPAQTISTEF AVKLKIDLAN RETSIFSYRE DPIVYEIHPT
760 770 780 790 800
KSFISGGSTI TGVGKNLNSV SVPRMVINVH EAGRNFTVAC QHRSNSEIIC
810 820 830 840 850
CTTPSLQQLN LQLPLKTKAF FMLDGILSKY FDLIYVHNPV FKPFEKPVMI
860 870 880 890 900
SMGNENVLEI KGNDIDPEAV KGEVLKVGNK SCENIHLHSE AVLCTVPNDL
910 920 930 940 950
LKLNSELNIE WKQAISSTVL GKVIVQPDQN FTGLIAGVVS ISTALLLLLG
960 970 980 990 1000
FFLWLKKRKQ IKDLGSELVR YDARVHTPHL DRLVSARSVS PTTEMVSNES
1010 1020 1030 1040 1050
VDYRATFPED QFPNSSQNGS CRQVQYPLTD MSPILTSGDS DISSPLLQNT
1060 1070 1080 1090 1100
VHIDLSALNP ELVQAVQHVV IGPSSLIVHF NEVIGRGHFG CVYHGTLLDN
1110 1120 1130 1140 1150
DGKKIHCAVK SLNRITDIGE VSQFLTEGII MKDFSHPNVL SLLGICLRSE
1160 1170 1180 1190 1200
GSPLVVLPYM KHGDLRNFIR NETHNPTVKD LIGFGLQVAK GMKYLASKKF
1210 1220 1230 1240 1250
VHRDLAARNC MLDEKFTVKV ADFGLARDMY DKEYYSVHNK TGAKLPVKWM
1260 1270 1280 1290 1300
ALESLQTQKF TTKSDVWSFG VLLWELMTRG APPYPDVNTF DITVYLLQGR
1310 1320 1330 1340 1350
RLLQPEYCPD PLYEVMLKCW HPKAEMRPSF SELVSRISAI FSTFIGEHYV
1360 1370 1380 1390
HVNATYVNVK CVAPYPSLLS SEDNADDEVD TRPASFWETS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1496 Collapse assay details

Data source: Panorama

Official Gene Symbol
MET
Peptide Modified Sequence
FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR
Modification Type
Deamidated (N)
Protein - Site of Modification
202
Peptide - Site of Modification
11
Peptide Start
192
Peptide End
217
CPTAC ID
CPTAC-1496
Peptide Molecular Mass
3,008.4821
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 27.2 29.2 13 26.8 29.4 13.4 38.2 41.4 18.7 15 15 15
y8 (1+) 40.2 14.3 4.5 40.9 15.5 6.1 57.3 21.1 7.6 15 15 15
y11 (1+) 72.8 28.5 23.5 69.1 32.9 28.4 100.4 43.5 36.9 15 15 15
sum 20.7 5.4 4.7 23.8 4.4 7.6 31.5 7 8.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-2784 Collapse assay details

Data source: Panorama

Official Gene Symbol
MET
Peptide Modified Sequence
DMYDKEY[+80.0]YSVHNK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
1228
Peptide End
1240
CPTAC ID
CPTAC-2784
Peptide Molecular Mass
1,770.6961
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 36.9 9.3 4.6 32.3 9.9 7.8 49 13.6 9.1 15 15 15
y7 (1+) 26.9 9.6 5.6 27.3 11 6.8 38.3 14.6 8.8 15 15 15
y5 (1+) 32.6 8.9 5.3 32.6 9.8 6.2 46.1 13.2 8.2 15 15 15
y3 (1+) 22.5 5.2 4.4 26.5 7 5.4 34.8 8.7 7 15 15 15
y2 (1+) 25.7 9.7 4.4 26.1 10.3 5.8 36.6 14.1 7.3 15 15 15
b2 (1+) 31.7 8 5.3 28.4 8.4 6.7 42.6 11.6 8.5 15 15 15
sum 26.4 7.8 3.3 26.7 8.4 5.2 37.5 11.5 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2816 Collapse assay details

Data source: Panorama

Official Gene Symbol
MET
Peptide Sequence
TEFTTALQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
418
Peptide End
426
CPTAC ID
CPTAC-2816
Peptide Molecular Mass
1,065.5455
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.5 2.5 2.3 7.8 3.1 7.3 10.8 4 7.7 15 15 15
y4 (1+) 8.7 4 4.9 8.5 3.6 7.2 12.2 5.4 8.7 15 15 15
y3 (1+) 7.8 3.8 5.5 13.3 3.9 7.8 15.4 5.4 9.5 15 15 15
sum 5.4 2.7 2.9 5.5 2.9 7.2 7.7 4 7.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments