FOLH1, CPTAC-1491 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FOLH1	FOLH, NAALAD1, PSM, PSMA

Sequence Length (AA)	Molecular Weight (Da)
750	84331

Protein Name
Glutamate carboxypeptidase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWNLLHETDS	AVATARRPRW	LCAGALVLAG	GFFLLGFLFG	WFIKSSNEAT
60	70	80	90	100
NITPKHNMKA	FLDELKAENI	KKFLYNFTQI	PHLAGTEQNF	QLAKQIQSQW
110	120	130	140	150
KEFGLDSVEL	AHYDVLLSYP	NKTHPNYISI	INEDGNEIFN	TSLFEPPPPG
160	170	180	190	200
YENVSDIVPP	FSAFSPQGMP	EGDLVYVNYA	RTEDFFKLER	DMKINCSGKI
210	220	230	240	250
VIARYGKVFR	GNKVKNAQLA	GAKGVILYSD	PADYFAPGVK	SYPDGWNLPG
260	270	280	290	300
GGVQRGNILN	LNGAGDPLTP	GYPANEYAYR	RGIAEAVGLP	SIPVHPIGYY
310	320	330	340	350
DAQKLLEKMG	GSAPPDSSWR	GSLKVPYNVG	PGFTGNFSTQ	KVKMHIHSTN
360	370	380	390	400
EVTRIYNVIG	TLRGAVEPDR	YVILGGHRDS	WVFGGIDPQS	GAAVVHEIVR
410	420	430	440	450
SFGTLKKEGW	RPRRTILFAS	WDAEEFGLLG	STEWAEENSR	LLQERGVAYI
460	470	480	490	500
NADSSIEGNY	TLRVDCTPLM	YSLVHNLTKE	LKSPDEGFEG	KSLYESWTKK
510	520	530	540	550
SPSPEFSGMP	RISKLGSGND	FEVFFQRLGI	ASGRARYTKN	WETNKFSGYP
560	570	580	590	600
LYHSVYETYE	LVEKFYDPMF	KYHLTVAQVR	GGMVFELANS	IVLPFDCRDY
610	620	630	640	650
AVVLRKYADK	IYSISMKHPQ	EMKTYSVSFD	SLFSAVKNFT	EIASKFSERL
660	670	680	690	700
QDFDKSNPIV	LRMMNDQLMF	LERAFIDPLG	LPDRPFYRHV	IYAPSSHNKY
710	720	730	740	750
AGESFPGIYD	ALFDIESKVD	PSKAWGEVKR	QIYVAAFTVQ	AAAETLSEVA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1491 Collapse assay details

Data source: Panorama

Official Gene Symbol: FOLH1
Peptide Modified Sequence: VPYNVGPGFTGN[+1.0]FSTQK
Modification Type: Deamidated (N)
Protein - Site of Modification: 336
Peptide - Site of Modification: 12
Peptide Start: 325
Peptide End: 341

CPTAC ID: CPTAC-1491
Peptide Molecular Mass: 1,812.8683
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	12.6	5.3	8.8	11.5	5.8	10	17.1	7.9	13.3	15	15	15
y5 (1+)	33.6	10.4	9.2	36.4	12.8	10.8	49.5	16.5	14.2	15	15	15
b6 (1+)	10.8	13.3	11.7	14.6	9.8	10.9	18.2	16.5	16	15	15	15
sum	7.3	4.3	5.1	11.3	5.2	7.3	13.5	6.7	8.9	15	15	15

FOLH1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1491 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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FOLH1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1491 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments