ENTPD2, CPTAC-1489 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ENTPD2	CD39L1

Sequence Length (AA)	Molecular Weight (Da)
495	53665

Protein Name
Ectonucleoside triphosphate diphosphohydrolase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGKVRSLLP	PLLLAAAGLA	GLLLLCVPTR	DVREPPALKY	GIVLDAGSSH
60	70	80	90	100
TSMFIYKWPA	DKENDTGIVG	QHSSCDVPGG	GISSYADNPS	GASQSLVGCL
110	120	130	140	150
EQALQDVPKE	RHAGTPLYLG	ATAGMRLLNL	TNPEASTSVL	MAVTHTLTQY
160	170	180	190	200
PFDFRGARIL	SGQEEGVFGW	VTANYLLENF	IKYGWVGRWF	RPRKGTLGAM
210	220	230	240	250
DLGGASTQIT	FETTSPAEDR	ASEVQLHLYG	QHYRVYTHSF	LCYGRDQVLQ
260	270	280	290	300
RLLASALQTH	GFHPCWPRGF	STQVLLGDVY	QSPCTMAQRP	QNFNSSARVS
310	320	330	340	350
LSGSSDPHLC	RDLVSGLFSF	SSCPFSRCSF	NGVFQPPVAG	NFVAFSAFFY
360	370	380	390	400
TVDFLRTSMG	LPVATLQQLE	AAAVNVCNQT	WAQLQARVPG	QRARLADYCA
410	420	430	440	450
GAMFVQQLLS	RGYGFDERAF	GGVIFQKKAA	DTAVGWALGY	MLNLTNLIPA
460	470	480	490	495
DPPGLRKGTD	FSSWVVLLLL	FASALLAALV	LLLRQVHSAK	LPSTI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1489 Collapse assay details

Data source: Panorama

Official Gene Symbol: ENTPD2
Peptide Modified Sequence: LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
Modification Type: Deamidated (N), Oxidation (M)
Protein - Site of Modification: 129, 141
Peptide - Site of Modification: 3, 15
Peptide Start: 127
Peptide End: 155

CPTAC ID: CPTAC-1489
Peptide Molecular Mass: 3,295.6435
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	13.6	8	5	11.8	8.5	5.7	18	11.7	7.6	15	15	15
y5 (1+)	9.3	1.9	4.1	10.1	2.5	4.2	13.7	3.1	5.9	15	15	15
y8 (1+)	16.6	4.5	7.6	17.5	6.4	7.3	24.1	7.8	10.5	15	15	15
sum	9	2	1.9	8.3	2.2	1.8	12.2	3	2.6	15	15	15

ENTPD2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1489 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ENTPD2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1489 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments