CPE, CPTAC-1486 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CPE	N/A

Sequence Length (AA)	Molecular Weight (Da)
476	53151

Protein Name
Carboxypeptidase E

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGRGGSALL	ALCGALAACG	WLLGAEAQEP	GAPAAGMRRR	RRLQQEDGIS
60	70	80	90	100
FEYHRYPELR	EALVSVWLQC	TAISRIYTVG	RSFEGRELLV	IELSDNPGVH
110	120	130	140	150
EPGEPEFKYI	GNMHGNEAVG	RELLIFLAQY	LCNEYQKGNE	TIVNLIHSTR
160	170	180	190	200
IHIMPSLNPD	GFEKAASQPG	ELKDWFVGRS	NAQGIDLNRN	FPDLDRIVYV
210	220	230	240	250
NEKEGGPNNH	LLKNMKKIVD	QNTKLAPETK	AVIHWIMDIP	FVLSANLHGG
260	270	280	290	300
DLVANYPYDE	TRSGSAHEYS	SSPDDAIFQS	LARAYSSFNP	AMSDPNRPPC
310	320	330	340	350
RKNDDDSSFV	DGTTNGGAWY	SVPGGMQDFN	YLSSNCFEIT	VELSCEKFPP
360	370	380	390	400
EETLKTYWED	NKNSLISYLE	QIHRGVKGFV	RDLQGNPIAN	ATISVEGIDH
410	420	430	440	450
DVTSAKDGDY	WRLLIPGNYK	LTASAPGYLA	ITKKVAVPYS	PAAGVDFELE
460	470	476
SFSERKEEEK	EELMEWWKMM	SETLNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1486 Collapse assay details

Data source: Panorama

Official Gene Symbol: CPE
Peptide Modified Sequence: GN[+1.0]ETIVNLIHSTR
Modification Type: Deamidated (N)
Protein - Site of Modification: 139
Peptide - Site of Modification: 2
Peptide Start: 138
Peptide End: 150

CPTAC ID: CPTAC-1486
Peptide Molecular Mass: 1,453.7525
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	9.7	7.5	7.8	11.9	5.9	9	15.4	9.5	11.9	15	15	15
y8 (2+)	10	6.4	11.6	13.6	7.6	12.2	16.9	9.9	16.8	15	15	15
y4 (1+)	11.3	4.9	4.9	10.2	5.2	5.3	15.2	7.1	7.2	15	15	15
sum	7	3.7	4.5	7	2.9	5.2	9.9	4.7	6.9	15	15	15

CPE

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1486 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CPE

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1486 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments