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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CPE N/A
Sequence Length (AA) Molecular Weight (Da)
476 53151
Protein Name
Carboxypeptidase E
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGRGGSALL ALCGALAACG WLLGAEAQEP GAPAAGMRRR RRLQQEDGIS
60 70 80 90 100
FEYHRYPELR EALVSVWLQC TAISRIYTVG RSFEGRELLV IELSDNPGVH
110 120 130 140 150
EPGEPEFKYI GNMHGNEAVG RELLIFLAQY LCNEYQKGNE TIVNLIHSTR
160 170 180 190 200
IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV
210 220 230 240 250
NEKEGGPNNH LLKNMKKIVD QNTKLAPETK AVIHWIMDIP FVLSANLHGG
260 270 280 290 300
DLVANYPYDE TRSGSAHEYS SSPDDAIFQS LARAYSSFNP AMSDPNRPPC
310 320 330 340 350
RKNDDDSSFV DGTTNGGAWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP
360 370 380 390 400
EETLKTYWED NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVEGIDH
410 420 430 440 450
DVTSAKDGDY WRLLIPGNYK LTASAPGYLA ITKKVAVPYS PAAGVDFELE
460 470 476
SFSERKEEEK EELMEWWKMM SETLNF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1486 Collapse assay details

Data source: Panorama

Official Gene Symbol
CPE
Peptide Modified Sequence
GN[+1.0]ETIVNLIHSTR
Modification Type
Deamidated (N)
Protein - Site of Modification
139
Peptide - Site of Modification
2
Peptide Start
138
Peptide End
150
CPTAC ID
CPTAC-1486
Peptide Molecular Mass
1,453.7525
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 9.7 7.5 7.8 11.9 5.9 9 15.4 9.5 11.9 15 15 15
y8 (2+) 10 6.4 11.6 13.6 7.6 12.2 16.9 9.9 16.8 15 15 15
y4 (1+) 11.3 4.9 4.9 10.2 5.2 5.3 15.2 7.1 7.2 15 15 15
sum 7 3.7 4.5 7 2.9 5.2 9.9 4.7 6.9 15 15 15

Additional Resources and Comments