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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TMX4 KIAA1162, TXNDC13
Sequence Length (AA) Molecular Weight (Da)
349 38952
Protein Name
Thioredoxin-related transmembrane protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGGRCGPQL TALLAAWIAA VAATAGPEEA ALPPEQSRVQ PMTASNWTLV
60 70 80 90 100
MEGEWMLKFY APWCPSCQQT DSEWEAFAKN GEILQISVGK VDVIQEPGLS
110 120 130 140 150
GRFFVTTLPA FFHAKDGIFR RYRGPGIFED LQNYILEKKW QSVEPLTGWK
160 170 180 190 200
SPASLTMSGM AGLFSISGKI WHLHNYFTVT LGIPAWCSYV FFVIATLVFG
210 220 230 240 250
LFMGLVLVVI SECFYVPLPR HLSERSEQNR RSEEAHRAEQ LQDAEEEKDD
260 270 280 290 300
SNEEENKDSL VDDEEEKEDL GDEDEAEEEE EEDNLAAGVD EERSEANDQG
310 320 330 340 349
PPGEDGVTRE EVEPEEAEEG ISEQPCPADT EVVEDSLRQR KSQHADKGL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1512 Collapse assay details

Data source: Panorama

Official Gene Symbol
TMX4
Peptide Modified Sequence
VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
Modification Type
Oxidation (M), Deamidated (N), Oxidation (M), Oxidation (M)
Protein - Site of Modification
42, 46, 51, 56
Peptide - Site of Modification
4, 8, 13, 18
Peptide Start
39
Peptide End
58
CPTAC ID
CPTAC-1512
Peptide Molecular Mass
2,399.0847
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 9.9 4.4 5.6 8.8 6 6.3 13.2 7.4 8.4 15 15 15
y7 (1+) 6.3 7 3.3 9 8.2 4.7 11 10.8 5.7 15 15 15
y6 (1+) 7.8 3 6.2 5.4 4.1 5.4 9.5 5.1 8.2 15 15 15
sum 2.8 3.1 3 4.2 3.4 3.4 5 4.6 4.5 15 15 15


Additional Resources and Comments