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Overview Data source: UniProt

Official Gene Symbol Other Aliases
F3 N/A
Sequence Length (AA) Molecular Weight (Da)
295 33068
Protein Name
Tissue factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
METPAWPRVP RPETAVARTL LLGWVFAQVA GASGTTNTVA AYNLTWKSTN
60 70 80 90 100
FKTILEWEPK PVNQVYTVQI STKSGDWKSK CFYTTDTECD LTDEIVKDVK
110 120 130 140 150
QTYLARVFSY PAGNVESTGS AGEPLYENSP EFTPYLETNL GQPTIQSFEQ
160 170 180 190 200
VGTKVNVTVE DERTLVRRNN TFLSLRDVFG KDLIYTLYYW KSSSSGKKTA
210 220 230 240 250
KTNTNEFLID VDKGENYCFS VQAVIPSRTV NRKSTDSPVE CMGQEKGEFR
260 270 280 290 295
EIFYIIGAVV FVVIILVIIL AISLHKCRKA GVGQSWKENS PLNVS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1479 Collapse assay details

Data source: Panorama

Official Gene Symbol
F3
Peptide Modified Sequence
N[+1.0]NTFLSLR
Modification Type
Deamidated (N)
Protein - Site of Modification
169
Peptide - Site of Modification
1
Peptide Start
169
Peptide End
176
CPTAC ID
CPTAC-1479
Peptide Molecular Mass
964.4978
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.9 4.6 4.5 6.1 4.8 4.7 8.5 6.6 6.5 15 15 15
y4 (1+) 6 3.4 4.2 10.2 5.2 4.3 11.8 6.2 6 15 15 15
y6 (1+) 3.8 6.3 6.2 4.7 6.8 6 6 9.3 8.6 15 15 15
sum 4.2 2.2 3.2 5.3 2.9 3.3 6.8 3.6 4.6 15 15 15

Additional Resources and Comments