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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLOD1 LLH, PLOD
Sequence Length (AA) Molecular Weight (Da)
727 83550
Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPLLLLALL GWLLLAEAKG DAKPEDNLLV LTVATKETEG FRRFKRSAQF
60 70 80 90 100
FNYKIQALGL GEDWNVEKGT SAGGGQKVRL LKKALEKHAD KEDLVILFAD
110 120 130 140 150
SYDVLFASGP RELLKKFRQA RSQVVFSAEE LIYPDRRLET KYPVVSDGKR
160 170 180 190 200
FLGSGGFIGY APNLSKLVAE WEGQDSDSDQ LFYTKIFLDP EKREQINITL
210 220 230 240 250
DHRCRIFQNL DGALDEVVLK FEMGHVRARN LAYDTLPVLI HGNGPTKLQL
260 270 280 290 300
NYLGNYIPRF WTFETGCTVC DEGLRSLKGI GDEALPTVLV GVFIEQPTPF
310 320 330 340 350
VSLFFQRLLR LHYPQKHMRL FIHNHEQHHK AQVEEFLAQH GSEYQSVKLV
360 370 380 390 400
GPEVRMANAD ARNMGADLCR QDRSCTYYFS VDADVALTEP NSLRLLIQQN
410 420 430 440 450
KNVIAPLMTR HGRLWSNFWG ALSADGYYAR SEDYVDIVQG RRVGVWNVPY
460 470 480 490 500
ISNIYLIKGS ALRGELQSSD LFHHSKLDPD MAFCANIRQQ DVFMFLTNRH
510 520 530 540 550
TLGHLLSLDS YRTTHLHNDL WEVFSNPEDW KEKYIHQNYT KALAGKLVET
560 570 580 590 600
PCPDVYWFPI FTEVACDELV EEMEHFGQWS LGNNKDNRIQ GGYENVPTID
610 620 630 640 650
IHMNQIGFER EWHKFLLEYI APMTEKLYPG YYTRAQFDLA FVVRYKPDEQ
660 670 680 690 700
PSLMPHHDAS TFTINIALNR VGVDYEGGGC RFLRYNCSIR APRKGWTLMH
710 720 727
PGRLTHYHEG LPTTRGTRYI AVSFVDP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1500 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD1
Peptide Modified Sequence
EQIN[+1.0]ITLDHR
Modification Type
Deamidated (N)
Protein - Site of Modification
197
Peptide - Site of Modification
4
Peptide Start
194
Peptide End
203
CPTAC ID
CPTAC-1500
Peptide Molecular Mass
1,238.6255
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.5 5 3.8 7.7 4.9 4 10.7 7 5.5 15 15 15
y7 (1+) 6.8 4.9 3.5 7 5.2 4 9.8 7.1 5.3 15 15 15
y5 (1+) 7.9 5.6 5.8 8.3 5.8 4.6 11.5 8.1 7.4 15 15 15
sum 3.5 3 3 3.7 3.4 2.7 5.1 4.5 4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-2232 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD1
Peptide Modified Sequence
YIHQN[+1.0]YTK
Modification Type
Deamidated (N)
Protein - Site of Modification
538
Peptide - Site of Modification
5
Peptide Start
534
Peptide End
541
CPTAC ID
CPTAC-2232
Peptide Molecular Mass
1,066.5084
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 3.8 2.3 1.2 4.8 2 1.2 6.1 3 1.7 15 15 15
y7 (2+) 4.8 2.3 1.3 5.4 2.4 1.6 7.2 3.3 2.1 15 15 15
y2 (1+) 5.3 2 2.2 4.9 2.2 2.7 7.2 3 3.5 15 15 15
sum 3.6 1.9 0.6 4.6 1.9 0.9 5.8 2.7 1.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-566 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD1
Peptide Sequence
LQLNYLGNYIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
248
Peptide End
259
CPTAC ID
CPTAC-566
Peptide Molecular Mass
1,462.7932
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 9.3 13.3 10.8 15.3 14.8 13.5 17.9 19.9 17.3 15 25 15
y9 (1+) 22.4 18 11.1 35.3 22.5 10.8 41.8 28.8 15.5 15 25 15
y7 (1+) 20.7 15.7 23.3 33.3 17 30.8 39.2 23.1 38.6 15 25 15
sum 13 11.2 11.6 22.5 14.5 16.1 26 18.3 19.8 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-567 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLOD1
Peptide Sequence
SEDYVDIVQGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
431
Peptide End
441
CPTAC ID
CPTAC-567
Peptide Molecular Mass
1,279.6044
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.7 15.9 10.2 18.8 17.6 14 22.7 23.7 17.3 15 25 15
y7 (1+) 17.5 16.2 26 19.6 17.2 44.7 26.3 23.6 51.7 15 25 15
y6 (1+) 9.8 11.6 8.6 15.8 14.1 11.7 18.6 18.3 14.5 15 25 15
sum 8.8 9.4 4.8 13.2 10.4 10.5 15.9 14 11.5 15 25 15

Additional Resources and Comments