PLOD3, CPTAC-1501 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PLOD3	N/A

Sequence Length (AA)	Molecular Weight (Da)
738	84785

Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSSGPGPRF	LLLLPLLLPP	AASASDRPRG	RDPVNPEKLL	VITVATAETE
60	70	80	90	100
GYLRFLRSAE	FFNYTVRTLG	LGEEWRGGDV	ARTVGGGQKV	RWLKKEMEKY
110	120	130	140	150
ADREDMIIMF	VDSYDVILAG	SPTELLKKFV	QSGSRLLFSA	ESFCWPEWGL
160	170	180	190	200
AEQYPEVGTG	KRFLNSGGFI	GFATTIHQIV	RQWKYKDDDD	DQLFYTRLYL
210	220	230	240	250
DPGLREKLSL	NLDHKSRIFQ	NLNGALDEVV	LKFDRNRVRI	RNVAYDTLPI
260	270	280	290	300
VVHGNGPTKL	QLNYLGNYVP	NGWTPEGGCG	FCNQDRRTLP	GGQPPPRVFL
310	320	330	340	350
AVFVEQPTPF	LPRFLQRLLL	LDYPPDRVTL	FLHNNEVFHE	PHIADSWPQL
360	370	380	390	400
QDHFSAVKLV	GPEEALSPGE	ARDMAMDLCR	QDPECEFYFS	LDADAVLTNL
410	420	430	440	450
QTLRILIEEN	RKVIAPMLSR	HGKLWSNFWG	ALSPDEYYAR	SEDYVELVQR
460	470	480	490	500
KRVGVWNVPY	ISQAYVIRGD	TLRMELPQRD	VFSGSDTDPD	MAFCKSFRDK
510	520	530	540	550
GIFLHLSNQH	EFGRLLATSR	YDTEHLHPDL	WQIFDNPVDW	KEQYIHENYS
560	570	580	590	600
RALEGEGIVE	QPCPDVYWFP	LLSEQMCDEL	VAEMEHYGQW	SGGRHEDSRL
610	620	630	640	650
AGGYENVPTV	DIHMKQVGYE	DQWLQLLRTY	VGPMTESLFP	GYHTKARAVM
660	670	680	690	700
NFVVRYRPDE	QPSLRPHHDS	STFTLNVALN	HKGLDYEGGG	CRFLRYDCVI
710	720	730	738
SSPRKGWALL	HPGRLTHYHE	GLPTTWGTRY	IMVSFVDP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-113 Collapse assay details

Data source: Panorama

Official Gene Symbol: PLOD3
Peptide Sequence: LVGPEEALSPGEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 359
Peptide End: 372

CPTAC ID: CPTAC-113
Peptide Molecular Mass: 1,423.7307
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (1+)	8.2	9.5	9.2	7.1	11.7	7	10.8	15.1	11.6	15	25	15
y8 (1+)	11.2	14.3	10	10.3	14.5	22.6	15.2	20.4	24.7	15	25	15
y7 (1+)	9.3	11	13.9	8.7	10.7	19.5	12.7	15.3	23.9	15	25	15
sum	6.5	9.1	6.7	6.5	10.2	14.5	9.2	13.7	16	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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