LAMP2, CPTAC-1494 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LAMP2	N/A

Sequence Length (AA)	Molecular Weight (Da)
410	44961

Protein Name
Lysosome-associated membrane glycoprotein 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVCFRLFPVP	GSGLVLVCLV	LGAVRSYALE	LNLTDSENAT	CLYAKWQMNF
60	70	80	90	100
TVRYETTNKT	YKTVTISDHG	TVTYNGSICG	DDQNGPKIAV	QFGPGFSWIA
110	120	130	140	150
NFTKAASTYS	IDSVSFSYNT	GDNTTFPDAE	DKGILTVDEL	LAIRIPLNDL
160	170	180	190	200
FRCNSLSTLE	KNDVVQHYWD	VLVQAFVQNG	TVSTNEFLCD	KDKTSTVAPT
210	220	230	240	250
IHTTVPSPTT	TPTPKEKPEA	GTYSVNNGND	TCLLATMGLQ	LNITQDKVAS
260	270	280	290	300
VININPNTTH	STGSCRSHTA	LLRLNSSTIK	YLDFVFAVKN	ENRFYLKEVN
310	320	330	340	350
ISMYLVNGSV	FSIANNNLSY	WDAPLGSSYM	CNKEQTVSVS	GAFQINTFDL
360	370	380	390	400
RVQPFNVTQG	KYSTAQDCSA	DDDNFLVPIA	VGAALAGVLI	LVLLAYFIGL
410
KHHHAGYEQF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1494 Collapse assay details

Data source: Panorama

Official Gene Symbol: LAMP2
Peptide Modified Sequence: VASVININPN[+1.0]TTHSTGSC[+57.0]R
Modification Type: Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification: 257, 265
Peptide - Site of Modification: 10, 18
Peptide Start: 248
Peptide End: 266

CPTAC ID: CPTAC-1494
Peptide Molecular Mass: 2,027.9695
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	8.1	9.5	5.5	7.8	6.8	7.1	11.2	11.7	9	15	15	15
y6 (1+)	7.3	6.9	5.2	8.2	7.1	7	11	9.9	8.7	15	15	15
y11 (1+)	8.3	9.1	7.9	11.3	8.9	8.1	14	12.7	11.3	15	15	15
sum	3.2	4.5	3.3	4.3	5.1	4.1	5.4	6.8	5.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-689 Collapse assay details

Data source: Panorama

Official Gene Symbol: LAMP2
Peptide Modified Sequence: VQPFN[+1.0]VTQGK
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 356
Peptide - Site of Modification: 5
Peptide Start: 352
Peptide End: 361

CPTAC ID: CPTAC-689
Peptide Molecular Mass: 1,117.5768
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang