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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CADM1 N/A
Sequence Length (AA) Molecular Weight (Da)
387 42737
Protein Name
CADM1 protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASVVLPSGS QCAAAAAAAA PPGLRLRLLL LLFSAAALIP TGDGQNLFTK
60 70 80 90 100
DVTVIEGEVA TISCQVNKSD DSVIQLLNPN RQTIYFRDFR PLKDSRFQLL
110 120 130 140 150
NFSSSELKVS LTNVSISDEG RYFCQLYTDP PQESYTTITV LVPPRNLMID
160 170 180 190 200
IQKDTAVEGE EIEVNCTAMA SKPATTIRWF KGNTELKGKS EVEEWSDMYT
210 220 230 240 250
VTSQLMLKVH KEDDGVPVIC QVEHPAVTGN LQTQRYLEVQ YKPQVHIQMT
260 270 280 290 300
YPLQGLTREG DALELTCEAI GKPQPVMVTW VRVDDEMPQH AVLSGPNLFI
310 320 330 340 350
NNLNKTDNGT YRCEASNIVG KAHSDYMLYV YDSRAGEEGS IRAVDHAVIG
360 370 380 387
GVVAVVVFAM LCLLIILGRY FARHKGLFSL TSSPRIK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1282 Collapse assay details

Data source: Panorama

Official Gene Symbol
CADM1
Peptide Modified Sequence
FQLLN[+1.0]FSSSELK
Modification Type
Deamidated (N)
Protein - Site of Modification
101
Peptide - Site of Modification
5
Peptide Start
97
Peptide End
108
CPTAC ID
CPTAC-1282
Peptide Molecular Mass
1,412.7187
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 52.4 7.5 5.2 66.6 9.1 5.4 84.7 11.8 7.5 15 15 15
y9 (1+) 40.8 6.2 3.3 46.5 6.1 4.9 61.9 8.7 5.9 15 15 15
y10 (1+) 36.4 6.7 4.9 39.9 6.4 6.8 54 9.3 8.4 15 15 15
sum 20 4.4 3.2 28.4 3.3 4.1 34.7 5.5 5.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1480 Collapse assay details

Data source: Panorama

Official Gene Symbol
CADM1
Peptide Modified Sequence
TDN[+1.0]GTYR
Modification Type
Deamidated (N)
Protein - Site of Modification
308
Peptide - Site of Modification
3
Peptide Start
306
Peptide End
312
CPTAC ID
CPTAC-1480
Peptide Molecular Mass
826.3457
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.3 13.1 8.9 10.7 8.9 9.9 13.5 15.8 13.3 15 15 15
y4 (1+) 3.4 4.5 3.8 3 3.7 4.8 4.5 5.8 6.1 15 15 15
y5 (1+) 2.5 2.6 3.6 2.4 3.4 3.4 3.5 4.3 5 15 15 15
sum 2.4 2.2 3.1 2 2.4 3 3.1 3.3 4.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-2210 Collapse assay details

Data source: Panorama

Official Gene Symbol
CADM1
Peptide Modified Sequence
VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K
Modification Type
Oxidation (M), Deamidated (N)
Protein - Site of Modification
287, 304
Peptide - Site of Modification
5, 22
Peptide Start
283
Peptide End
305
CPTAC ID
CPTAC-2210
Peptide Molecular Mass
2,581.2483
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b11 (2+) 40.5 21.8 58.6 40.2 20.2 45 57.1 29.7 73.9 15 15 15
b15 (2+) 10.5 4.9 7 15.5 6.2 9.1 18.7 7.9 11.5 15 15 15
b16 (2+) 9 7.2 6.3 10.3 7.1 5.8 13.7 10.1 8.6 15 15 15
sum 6.6 4.4 4.8 10.7 4.5 5 12.6 6.3 6.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1283 Collapse assay details

Data source: Panorama

Official Gene Symbol
CADM1
Peptide Modified Sequence
VSLTN[+1.0]VSISDEGR
Modification Type
Deamidated (N)
Protein - Site of Modification
113
Peptide - Site of Modification
5
Peptide Start
109
Peptide End
121
CPTAC ID
CPTAC-1283
Peptide Molecular Mass
1,376.6783
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 36.5 22.4 25 50.1 23.5 24.1 62 32.5 34.7 9 15 15
y7 (1+) 35.9 11.6 10.5 46.7 15.2 8.9 58.9 19.1 13.8 15 15 15
y10 (1+) 61.9 29.3 10 67.4 26.9 12.2 91.5 39.8 15.8 14 15 15
sum 26.7 10.4 4.4 29.9 11.6 4.2 40.1 15.6 6.1 15 15 15

Additional Resources and Comments