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Overview Data source: UniProt

Official Gene Symbol Other Aliases
POFUT1 FUT12, KIAA0180
Sequence Length (AA) Molecular Weight (Da)
388 43956
Protein Name
GDP-fucose protein O-fucosyltransferase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAAAWARPL SVSFLLLLLP LPGMPAGSWD PAGYLLYCPC MGRFGNQADH
60 70 80 90 100
FLGSLAFAKL LNRTLAVPPW IEYQHHKPPF TNLHVSYQKY FKLEPLQAYH
110 120 130 140 150
RVISLEDFME KLAPTHWPPE KRVAYCFEVA AQRSPDKKTC PMKEGNPFGP
160 170 180 190 200
FWDQFHVSFN KSELFTGISF SASYREQWSQ RFSPKEHPVL ALPGAPAQFP
210 220 230 240 250
VLEEHRPLQK YMVWSDEMVK TGEAQIHAHL VRPYVGIHLR IGSDWKNACA
260 270 280 290 300
MLKDGTAGSH FMASPQCVGY SRSTAAPLTM TMCLPDLKEI QRAVKLWVRS
310 320 330 340 350
LDAQSVYVAT DSESYVPELQ QLFKGKVKVV SLKPEVAQVD LYILGQADHF
360 370 380 388
IGNCVSSFTA FVKRERDLQG RPSSFFGMDR PPKLRDEF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1506 Collapse assay details

Data source: Panorama

Official Gene Symbol
POFUT1
Peptide Modified Sequence
EGNPFGPFWDQFHVSFN[+1.0]K
Modification Type
Deamidated (N)
Protein - Site of Modification
160
Peptide - Site of Modification
17
Peptide Start
144
Peptide End
161
CPTAC ID
CPTAC-1506
Peptide Molecular Mass
2,152.9643
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12 1.7 1.8 17.5 2.3 2.2 21.2 2.9 2.8 15 15 15
y13 (2+) 3.9 1.8 2.3 4.6 2.7 2.1 6 3.2 3.1 15 15 15
y5 (1+) 4.7 1.8 1.7 6.4 1.9 2.1 7.9 2.6 2.7 15 15 15
sum 4.9 1.1 1.2 5.9 1.6 1.2 7.7 1.9 1.7 15 15 15

Additional Resources and Comments