POFUT1, CPTAC-1506 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
POFUT1	FUT12, KIAA0180

Sequence Length (AA)	Molecular Weight (Da)
388	43956

Protein Name
GDP-fucose protein O-fucosyltransferase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGAAAWARPL	SVSFLLLLLP	LPGMPAGSWD	PAGYLLYCPC	MGRFGNQADH
60	70	80	90	100
FLGSLAFAKL	LNRTLAVPPW	IEYQHHKPPF	TNLHVSYQKY	FKLEPLQAYH
110	120	130	140	150
RVISLEDFME	KLAPTHWPPE	KRVAYCFEVA	AQRSPDKKTC	PMKEGNPFGP
160	170	180	190	200
FWDQFHVSFN	KSELFTGISF	SASYREQWSQ	RFSPKEHPVL	ALPGAPAQFP
210	220	230	240	250
VLEEHRPLQK	YMVWSDEMVK	TGEAQIHAHL	VRPYVGIHLR	IGSDWKNACA
260	270	280	290	300
MLKDGTAGSH	FMASPQCVGY	SRSTAAPLTM	TMCLPDLKEI	QRAVKLWVRS
310	320	330	340	350
LDAQSVYVAT	DSESYVPELQ	QLFKGKVKVV	SLKPEVAQVD	LYILGQADHF
360	370	380	388
IGNCVSSFTA	FVKRERDLQG	RPSSFFGMDR	PPKLRDEF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1506 Collapse assay details

Data source: Panorama

Official Gene Symbol: POFUT1
Peptide Modified Sequence: EGNPFGPFWDQFHVSFN[+1.0]K
Modification Type: Deamidated (N)
Protein - Site of Modification: 160
Peptide - Site of Modification: 17
Peptide Start: 144
Peptide End: 161

CPTAC ID: CPTAC-1506
Peptide Molecular Mass: 2,152.9643
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	12	1.7	1.8	17.5	2.3	2.2	21.2	2.9	2.8	15	15	15
y13 (2+)	3.9	1.8	2.3	4.6	2.7	2.1	6	3.2	3.1	15	15	15
y5 (1+)	4.7	1.8	1.7	6.4	1.9	2.1	7.9	2.6	2.7	15	15	15
sum	4.9	1.1	1.2	5.9	1.6	1.2	7.7	1.9	1.7	15	15	15

POFUT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1506 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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POFUT1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1506 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments