GGT7, CPTAC-1493 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GGT7	GGTL3, GGTL5

Sequence Length (AA)	Molecular Weight (Da)
662	70467

Protein Name
Gamma-glutamyltransferase 7

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAENEASQE	SALGAYSPVD	YMSITSFPRL	PEDEPAPAAP	LRGRKDEDAF
60	70	80	90	100
LGDPDTDPDS	FLKSARLQRL	PSSSSEMGSQ	DGSPLRETRK	DPFSAAAAEC
110	120	130	140	150
SCRQDGLTVI	VTACLTFATG	VTVALVMQIY	FGDPQIFQQG	AVVTDAARCT
160	170	180	190	200
SLGIEVLSKQ	GSSVDAAVAA	ALCLGIVAPH	SSGLGGGGVM	LVHDIRRNES
210	220	230	240	250
HLIDFRESAP	GALREETLQR	SWETKPGLLV	GVPGMVKGLH	EAHQLYGRLP
260	270	280	290	300
WSQVLAFAAA	VAQDGFNVTH	DLARALAEQL	PPNMSERFRE	TFLPSGRPPL
310	320	330	340	350
PGSLLHRPDL	AEVLDVLGTS	GPAAFYAGGN	LTLEMVAEAQ	HAGGVITEED
360	370	380	390	400
FSNYSALVEK	PVCGVYRGHL	VLSPPPPHTG	PALISALNIL	EGFNLTSLVS
410	420	430	440	450
REQALHWVAE	TLKIALALAS	RLGDPVYDST	ITESMDDMLS	KVEAAYLRGH
460	470	480	490	500
INDSQAAPAP	LLPVYELDGA	PTAAQVLIMG	PDDFIVAMVS	SLNQPFGSGL
510	520	530	540	550
ITPSGILLNS	QMLDFSWPNR	TANHSAPSLE	NSVQPGKRPL	SFLLPTVVRP
560	570	580	590	600
AEGLCGTYLA	LGANGAARGL	SGLTQVLLNV	LTLNRNLSDS	LARGRLHPDL
610	620	630	640	650
QSNLLQVDSE	FTEEEIEFLE	ARGHHVEKVD	VLSWVHGSRR	TNNFIIAVKD
660	662
PRSPDAAGAT	IL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1493 Collapse assay details

Data source: Panorama

Official Gene Symbol: GGT7
Peptide Modified Sequence: TAN[+1.0]HSAPSLENSVQPGK
Modification Type: Deamidated (N)
Protein - Site of Modification: 523
Peptide - Site of Modification: 3
Peptide Start: 521
Peptide End: 537

CPTAC ID: CPTAC-1493
Peptide Molecular Mass: 1,736.8329
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	8	7.3	7.8	9.5	8.2	8.8	12.4	11	11.8	15	15	15
y3 (1+)	4.2	3.4	2.8	8.7	6.2	2.9	9.7	7.1	4	15	15	15
b6 (1+)	9.5	8.2	9.3	10.6	8.3	8.2	14.2	11.7	12.4	15	15	15
sum	3.1	2.9	2.8	7.3	5	2.5	7.9	5.8	3.8	15	15	15

GGT7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1493 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GGT7

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1493 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments