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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SUN1 KIAA0810, UNC84A
Sequence Length (AA) Molecular Weight (Da)
812 90064
Protein Name
SUN domain-containing protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDFSRLHMYS PPQCVPENTG YTYALSSSYS SDALDFETEH KLDPVFDSPR
60 70 80 90 100
MSRRSLRLAT TACTLGDGEA VGADSGTSSA VSLKNRAART TKQRRSTNKS
110 120 130 140 150
AFSINHVSRQ VTSSGVSHGG TVSLQDAVTR RPPVLDESWI REQTTVDHFW
160 170 180 190 200
GLDDDGDLKG GNKAAIQGNG DVGAAAATAH NGFSCSNCSM LSERKDVLTA
210 220 230 240 250
HPAAPGPVSR VYSRDRNQKC DDCKGKRHLD AHPGRAGTLW HIWACAGYFL
260 270 280 290 300
LQILRRIGAV GQAVSRTAWS ALWLAVVAPG KAASGVFWWL GIGWYQFVTL
310 320 330 340 350
ISWLNVFLLT RCLRNICKFL VLLIPLFLLL AGLSLRGQGN FFSFLPVLNW
360 370 380 390 400
ASMHRTQRVD DPQDVFKPTT SRLKQPLQGD SEAFPWHWMS GVEQQVASLS
410 420 430 440 450
GQCHHHGENL RELTTLLQKL QARVDQMEGG AAGPSASVRD AVGQPPRETD
460 470 480 490 500
FMAFHQEHEV RMSHLEDILG KLREKSEAIQ KELEQTKQKT ISAVGEQLLP
510 520 530 540 550
TVEHLQLELD QLKSELSSWR HVKTGCETVD AVQERVDVQV REMVKLLFSE
560 570 580 590 600
DQQGGSLEQL LQRFSSQFVS KGDLQTMLRD LQLQILRNVT HHVSVTKQLP
610 620 630 640 650
TSEAVVSAVS EAGASGITEA QARAIVNSAL KLYSQDKTGM VDFALESGGG
660 670 680 690 700
SILSTRCSET YETKTALMSL FGIPLWYFSQ SPRVVIQPDI YPGNCWAFKG
710 720 730 740 750
SQGYLVVRLS MMIHPAAFTL EHIPKTLSPT GNISSAPKDF AVYGLENEYQ
760 770 780 790 800
EEGQLLGQFT YDQDGESLQM FQALKRPDDT AFQIVELRIF SNWGHPEYTC
810 812
LYRFRVHGEP VK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1509 Collapse assay details

Data source: Panorama

Official Gene Symbol
SUN1
Peptide Modified Sequence
N[+1.0]VTHHVSVTK
Modification Type
Deamidated (N)
Protein - Site of Modification
588
Peptide - Site of Modification
1
Peptide Start
588
Peptide End
597
CPTAC ID
CPTAC-1509
Peptide Molecular Mass
1,121.5829
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 0.9 1.2 1.7 0.9 1.4 2 1.3 1.8 2.6 15 15 15
y7 (2+) 3.5 3.5 2.1 4 3.4 2.9 5.3 4.9 3.6 15 15 15
y8 (2+) 1.8 1.5 1.1 1.9 2 2.6 2.6 2.5 2.8 15 15 15
sum 1.3 1.1 1.1 1.3 1.6 2.2 1.8 1.9 2.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1297 Collapse assay details

Data source: Panorama

Official Gene Symbol
SUN1
Peptide Modified Sequence
TLSPTGN[+1.0]ISSAPK
Modification Type
Deamidated (N)
Protein - Site of Modification
732
Peptide - Site of Modification
7
Peptide Start
726
Peptide End
738
CPTAC ID
CPTAC-1297
Peptide Molecular Mass
1,272.6561
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 65.7 31.3 22.3 74.9 38.9 22.5 99.6 49.9 31.7 15 15 15
y10 (2+) 57.1 13.6 21.8 67 18.7 21.6 88 23.1 30.7 15 15 15
b3 (1+) 57.1 25.9 26 80.5 18.8 23.4 98.7 32 35 15 15 15
sum 23.1 9.6 11.7 36.6 13.8 12.8 43.3 16.8 17.3 15 15 15


Additional Resources and Comments