SUN1, CPTAC-1509 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SUN1	KIAA0810, UNC84A

Sequence Length (AA)	Molecular Weight (Da)
812	90064

Protein Name
SUN domain-containing protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDFSRLHMYS	PPQCVPENTG	YTYALSSSYS	SDALDFETEH	KLDPVFDSPR
60	70	80	90	100
MSRRSLRLAT	TACTLGDGEA	VGADSGTSSA	VSLKNRAART	TKQRRSTNKS
110	120	130	140	150
AFSINHVSRQ	VTSSGVSHGG	TVSLQDAVTR	RPPVLDESWI	REQTTVDHFW
160	170	180	190	200
GLDDDGDLKG	GNKAAIQGNG	DVGAAAATAH	NGFSCSNCSM	LSERKDVLTA
210	220	230	240	250
HPAAPGPVSR	VYSRDRNQKC	DDCKGKRHLD	AHPGRAGTLW	HIWACAGYFL
260	270	280	290	300
LQILRRIGAV	GQAVSRTAWS	ALWLAVVAPG	KAASGVFWWL	GIGWYQFVTL
310	320	330	340	350
ISWLNVFLLT	RCLRNICKFL	VLLIPLFLLL	AGLSLRGQGN	FFSFLPVLNW
360	370	380	390	400
ASMHRTQRVD	DPQDVFKPTT	SRLKQPLQGD	SEAFPWHWMS	GVEQQVASLS
410	420	430	440	450
GQCHHHGENL	RELTTLLQKL	QARVDQMEGG	AAGPSASVRD	AVGQPPRETD
460	470	480	490	500
FMAFHQEHEV	RMSHLEDILG	KLREKSEAIQ	KELEQTKQKT	ISAVGEQLLP
510	520	530	540	550
TVEHLQLELD	QLKSELSSWR	HVKTGCETVD	AVQERVDVQV	REMVKLLFSE
560	570	580	590	600
DQQGGSLEQL	LQRFSSQFVS	KGDLQTMLRD	LQLQILRNVT	HHVSVTKQLP
610	620	630	640	650
TSEAVVSAVS	EAGASGITEA	QARAIVNSAL	KLYSQDKTGM	VDFALESGGG
660	670	680	690	700
SILSTRCSET	YETKTALMSL	FGIPLWYFSQ	SPRVVIQPDI	YPGNCWAFKG
710	720	730	740	750
SQGYLVVRLS	MMIHPAAFTL	EHIPKTLSPT	GNISSAPKDF	AVYGLENEYQ
760	770	780	790	800
EEGQLLGQFT	YDQDGESLQM	FQALKRPDDT	AFQIVELRIF	SNWGHPEYTC
810	812
LYRFRVHGEP	VK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1509 Collapse assay details

Data source: Panorama

Official Gene Symbol: SUN1
Peptide Modified Sequence: N[+1.0]VTHHVSVTK
Modification Type: Deamidated (N)
Protein - Site of Modification: 588
Peptide - Site of Modification: 1
Peptide Start: 588
Peptide End: 597

CPTAC ID: CPTAC-1509
Peptide Molecular Mass: 1,121.5829
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	0.9	1.2	1.7	0.9	1.4	2	1.3	1.8	2.6	15	15	15
y7 (2+)	3.5	3.5	2.1	4	3.4	2.9	5.3	4.9	3.6	15	15	15
y8 (2+)	1.8	1.5	1.1	1.9	2	2.6	2.6	2.5	2.8	15	15	15
sum	1.3	1.1	1.1	1.3	1.6	2.2	1.8	1.9	2.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1297 Collapse assay details

Data source: Panorama

Official Gene Symbol: SUN1
Peptide Modified Sequence: TLSPTGN[+1.0]ISSAPK
Modification Type: Deamidated (N)
Protein - Site of Modification: 732
Peptide - Site of Modification: 7
Peptide Start: 726
Peptide End: 738

CPTAC ID: CPTAC-1297
Peptide Molecular Mass: 1,272.6561
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang