PIGT, CPTAC-1499 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PIGT	N/A

Sequence Length (AA)	Molecular Weight (Da)
578	65700

Protein Name
GPI transamidase component PIG-T

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAMPLALL	VLLLLGPGGW	CLAEPPRDSL	REELVITPLP	SGDVAATFQF
60	70	80	90	100
RTRWDSELQR	EGVSHYRLFP	KALGQLISKY	SLRELHLSFT	QGFWRTRYWG
110	120	130	140	150
PPFLQAPSGA	ELWVWFQDTV	TDVDKSWKEL	SNVLSGIFCA	SLNFIDSTNT
160	170	180	190	200
VTPTASFKPL	GLANDTDHYF	LRYAVLPREV	VCTENLTPWK	KLLPCSSKAG
210	220	230	240	250
LSVLLKADRL	FHTSYHSQAV	HIRPVCRNAR	CTSISWELRQ	TLSVVFDAFI
260	270	280	290	300
TGQGKKDWSL	FRMFSRTLTE	PCPLASESRV	YVDITTYNQD	NETLEVHPPP
310	320	330	340	350
TTTYQDVILG	TRKTYAIYDL	LDTAMINNSR	NLNIQLKWKR	PPENEAPPVP
360	370	380	390	400
FLHAQRYVSG	YGLQKGELST	LLYNTHPYRA	FPVLLLDTVP	WYLRLYVHTL
410	420	430	440	450
TITSKGKENK	PSYIHYQPAQ	DRLQPHLLEM	LIQLPANSVT	KVSIQFERAL
460	470	480	490	500
LKWTEYTPDP	NHGFYVSPSV	LSALVPSMVA	AKPVDWEESP	LFNSLFPVSD
510	520	530	540	550
GSNYFVRLYT	EPLLVNLPTP	DFSMPYNVIC	LTCTVVAVCY	GSFYNLLTRT
560	570	578
FHIEEPRTGG	LAKRLANLIR	RARGVPPL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1499 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIGT
Peptide Modified Sequence: TYAIYDLLDTAM[+16.0]IN[+1.0]NSR
Modification Type: Oxidation (M), Deamidated (N)
Protein - Site of Modification: 325, 327
Peptide - Site of Modification: 12, 14
Peptide Start: 314
Peptide End: 330

CPTAC ID: CPTAC-1499
Peptide Molecular Mass: 1,989.9354
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8-64 (1+)	12.4	8.9	5.2	15.7	10.6	5.4	20	13.8	7.5	15	15	15
y6 (1+)	15.6	6.8	6.8	13.3	7.8	8.6	20.5	10.3	11	15	15	15
y5 (1+)	24.8	6.9	6.2	36.6	8.5	4.2	44.2	10.9	7.5	15	15	15
sum	15.1	4.5	3.9	15.7	6.1	3.5	21.8	7.6	5.2	15	15	15

PIGT

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1499 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

PIGT

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1499 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments