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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
THY1 N/A
Sequence Length (AA) Molecular Weight (Da)
161 17935
Protein Name
Thy-1 membrane glycoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNLAISIALL LTVLQVSRGQ KVTSLTACLV DQSLRLDCRH ENTSSSPIQY
60 70 80 90 100
EFSLTRETKK HVLFGTVGVP EHTYRSRTNF TSKYNMKVLY LSAFTSKDEG
110 120 130 140 150
TYTCALHHSG HSPPISSQNV TVLRDKLVKC EGISLLAQNT SWLLLLLLSL
160 161
SLLQATDFMS L

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1511 Collapse assay details

Data source: Panorama

Official Gene Symbol
THY1
Peptide Modified Sequence
DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR
Modification Type
Carbamidomethyl (C), Deamidated (N)
Protein - Site of Modification
104, 119
Peptide - Site of Modification
7, 22
Peptide Start
98
Peptide End
124
CPTAC ID
CPTAC-1511
Peptide Molecular Mass
2,963.3832
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 7 8.8 7.8 8.6 9.1 8.9 11.1 12.7 11.8 15 15 15
y9 (1+) 8.9 7.4 7.7 8.4 7.3 8.5 12.2 10.4 11.5 15 15 15
y6 (1+) 6.3 5.6 6.6 7.7 6.4 7.6 9.9 8.5 10.1 15 15 15
sum 4.4 3.9 5.4 4.4 4.1 5.6 6.2 5.7 7.8 15 15 15


Additional Resources and Comments