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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ALCAM MEMD
Sequence Length (AA) Molecular Weight (Da)
583 65102
Protein Name
CD166 antigen
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESKGASSCR LLFCLLISAT VFRPGLGWYT VNSAYGDTII IPCRLDVPQN
60 70 80 90 100
LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL NLSENYTLSI
110 120 130 140 150
SNARISDEKR FVCMLVTEDN VFEAPTIVKV FKQPSKPEIV SKALFLETEQ
160 170 180 190 200
LKKLGDCISE DSYPDGNITW YRNGKVLHPL EGAVVIIFKK EMDPVTQLYT
210 220 230 240 250
MTSTLEYKTT KADIQMPFTC SVTYYGPSGQ KTIHSEQAVF DIYYPTEQVT
260 270 280 290 300
IQVLPPKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL
310 320 330 340 350
TDVRRNATGD YKCSLIDKKS MIASTAITVH YLDLSLNPSG EVTRQIGDAL
360 370 380 390 400
PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE
410 420 430 440 450
GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT
460 470 480 490 500
GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV
510 520 530 540 550
SAISIPEHDE ADEISDENRE KVNDQAKLIV GIVVGLLLAA LVAGVVYWLY
560 570 580 583
MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6040 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALCAM
Peptide Sequence
ALFLETEQLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
143
Peptide End
152
CPTAC ID
CPTAC-6040
Peptide Molecular Mass
1,190.6547
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 13.4 5.6 6.8 16.8 8.3 7.2 21.5 10 9.9 15 15 15
y4 (1+) 22.7 8 8.5 23.5 7.1 8.6 32.7 10.7 12.1 15 15 15
y5 (1+) 17.9 5.6 6.4 20.8 7.9 8.9 27.4 9.7 11 15 15 15
y6 (1+) 14.5 6.1 6.3 16.4 5.7 6.6 21.9 8.3 9.1 15 15 15
y7 (1+) 17.3 5.6 6.5 20.3 6.2 8.5 26.7 8.4 10.7 15 15 15
y8 (1+) 13.8 5.8 5.6 17.1 6.1 9.9 22 8.4 11.4 15 15 15
y8 (2+) 12.6 6.9 8.1 19.6 5 6.6 23.3 8.5 10.4 15 15 15
sum 13.7 5.8 6.1 17 6.1 7.4 21.8 8.4 9.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1502 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALCAM
Peptide Modified Sequence
LN[+1.0]LSEN[+1.0]YTLSISNAR
Modification Type
Deamidated (N), Deamidated (N)
Protein - Site of Modification
91, 95
Peptide - Site of Modification
2, 6
Peptide Start
90
Peptide End
104
CPTAC ID
CPTAC-1502
Peptide Molecular Mass
1,695.8315
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 47.7 14.4 5.1 45 17.1 9 65.6 22.4 10.3 15 15 15
y7 (1+) 79.9 45.9 10.2 97 51.6 10.1 125.7 69.1 14.4 15 15 15
y8 (1+) 105.9 33.6 9.6 107.3 39.6 9.9 150.8 51.9 13.8 15 15 15
sum 46.3 16 5.7 60 24 7.5 75.8 28.8 9.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1503 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALCAM
Peptide Modified Sequence
TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR
Modification Type
Carbamidomethyl (C), Deamidated (N)
Protein - Site of Modification
435, 457
Peptide - Site of Modification
4, 26
Peptide Start
432
Peptide End
468
CPTAC ID
CPTAC-1503
Peptide Molecular Mass
4,100.0313
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 23.9 9.1 8.2 19.3 7.7 8.5 30.7 11.9 11.8 15 15 15
y6 (1+) 11.4 4.6 3.8 15 5.6 3.2 18.8 7.2 5 15 15 15
y8 (1+) 21.9 12.1 4.8 23 11.2 5.9 31.8 16.5 7.6 15 15 15
sum 8.4 4.3 3.5 11.6 4.9 2.8 14.3 6.5 4.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3640 Collapse assay details

Data source: Panorama

Official Gene Symbol
Alcam
Peptide Sequence
SVQYDDVPEYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
77
Peptide End
87
CPTAC ID
non-CPTAC-3640
Peptide Molecular Mass
1,341.6089
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 11.5 6.3 4.7 20.8 8.3 7 23.8 10.4 8.4 15 15 15
b3 (1+) 18.9 10.7 6.4 17.9 9 7.7 26 14 10 15 15 15
y9 (2+) 13.2 6.3 5.2 15 8.3 5 20 10.4 7.2 15 15 15
y2 (1+) 11.4 8.7 6.9 22.8 10.9 6.7 25.5 13.9 9.6 15 15 15
y4 (1+) 12.1 7 4.1 10.6 9.4 5.1 16.1 11.7 6.5 15 15 15
sum 3.5 3.6 1.7 7.9 5.2 3.6 8.6 6.3 4 15 15 15


Additional Resources and Comments