DPY19L4, CPTAC-1488 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DPY19L4	N/A

Sequence Length (AA)	Molecular Weight (Da)
723	83756

Protein Name
Probable C-mannosyltransferase DPY19L4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAEEEGPPVE	LRQRKKPKSS	ENKESAKEEK	ISDIPIPERA	PKHVLFQRFA
60	70	80	90	100
KIFIGCLAAV	TSGMMYALYL	SAYHERKFWF	SNRQELEREI	TFQGDSAIYY
110	120	130	140	150
SYYKDMLKAP	SFERGVYELT	HNNKTVSLKT	INAVQQMSLY	PELIASILYQ
160	170	180	190	200
ATGSNEIIEP	VYFYIGIVFG	LQGIYVTALF	VTSWLMSGTW	LAGMLTVAWF
210	220	230	240	250
VINRVDTTRI	EYSIPLRENW	ALPYFACQIA	ALTGYLKSNL	NTYGERFCYL
260	270	280	290	300
LMSASTYTFM	MMWEYSHYLL	FLQAISLFLL	DTFSVEQSDK	VYEVYKIYIF
310	320	330	340	350
SLFLGYLLQF	ENPALLVSPL	LSLVAALMLA	KCLQLNVKKG	SFVAKIIKVI
360	370	380	390	400
NFYLVCTLTI	TLNIIMKMFV	PHKENGHMLK	FLEVKFGLNM	TKNFTMNWLL
410	420	430	440	450
CQESLQAPSQ	DFFLRLTQSS	LLPFYILVLI	ICFLSMLQVI	FRRINGKSLK
460	470	480	490	500
ETVTLEDGRI	GERPEIIYHV	IHTILLGSLA	MVIEGLKYIW	IPYVCMLAAF
510	520	530	540	550
GVCSPELWMT	LFKWLRLRTV	HPILLALILS	MAVPTIIGLS	LWKEFFPRLM
560	570	580	590	600
TELMELQEFY	DPDTVELMTW	IKRQAPVAAV	FAGSPQLMGA	IKLCTGWMVT
610	620	630	640	650
SLPLYNDDDL	LKRNENIYQI	YSKRSAEDIY	KILTSYKANY	LIVEDAICNE
660	670	680	690	700
VGPMRGCRVK	DLLDIANGHM	VCEEGDKLTY	SKYGRFCHEV	KINYSPYVNY
710	720	723
FTRVYWNRSY	FVYKINTVIS	FQS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1488 Collapse assay details

Data source: Panorama

Official Gene Symbol: DPY19L4
Peptide Modified Sequence: GVYELTHNN[+1.0]K
Modification Type: Deamidated (N)
Protein - Site of Modification: 123
Peptide - Site of Modification: 9
Peptide Start: 115
Peptide End: 124

CPTAC ID: CPTAC-1488
Peptide Molecular Mass: 1,174.5619
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	32.7	11.9	7.8	64.6	12.9	8.7	72.4	17.6	11.7	15	15	15
y6 (1+)	11.1	7.8	8.6	20.2	8.1	9.5	23	11.2	12.8	15	15	15
y5 (1+)	15.6	10.7	11.4	32.2	11.4	13.8	35.8	15.6	17.9	15	15	15
sum	15.4	6.1	4	32.6	6.6	4.3	36.1	9	5.9	15	15	15

DPY19L4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1488 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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DPY19L4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1488 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments