BPIFB1, CPTAC-1490 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BPIFB1	C20orf114, LPLUNC1

Sequence Length (AA)	Molecular Weight (Da)
484	52442

Protein Name
BPI fold-containing family B member 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGPWTFTLL	CGLLAATLIQ	ATLSPTAVLI	LGPKVIKEKL	TQELKDHNAT
60	70	80	90	100
SILQQLPLLS	AMREKPAGGI	PVLGSLVNTV	LKHIIWLKVI	TANILQLQVK
110	120	130	140	150
PSANDQELLV	KIPLDMVAGF	NTPLVKTIVE	FHMTTEAQAT	IRMDTSASGP
160	170	180	190	200
TRLVLSDCAT	SHGSLRIQLL	HKLSFLVNAL	AKQVMNLLVP	SLPNLVKNQL
210	220	230	240	250
CPVIEASFNG	MYADLLQLVK	VPISLSIDRL	EFDLLYPAIK	GDTIQLYLGA
260	270	280	290	300
KLLDSQGKVT	KWFNNSAASL	TMPTLDNIPF	SLIVSQDVVK	AAVAAVLSPE
310	320	330	340	350
EFMVLLDSVL	PESAHRLKSS	IGLINEKAAD	KLGSTQIVKI	LTQDTPEFFI
360	370	380	390	400
DQGHAKVAQL	IVLEVFPSSE	ALRPLFTLGI	EASSEAQFYT	KGDQLILNLN
410	420	430	440	450
NISSDRIQLM	NSGIGWFQPD	VLKNIITEII	HSILLPNQNG	KLRSGVPVSL
460	470	480	484
VKALGFEAAE	SSLTKDALVL	TPASLWKPSS	PVSQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1490 Collapse assay details

Data source: Panorama

Official Gene Symbol: BPIFB1
Peptide Modified Sequence: DHN[+1.0]ATSILQQLPLLSAM[+16.0]R
Modification Type: Deamidated (N), Oxidation (M)
Protein - Site of Modification: 48, 62
Peptide - Site of Modification: 3, 17
Peptide Start: 46
Peptide End: 63

CPTAC ID: CPTAC-1490
Peptide Molecular Mass: 2,024.0361
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	3.1	3.7	5.7	3.2	3.9	6.2	4.5	5.4	8.4	15	15	15
b8 (1+)	3.4	3.5	4.5	3.7	3.6	3.5	5	5	5.7	15	15	15
y7 (1+)	2.5	2.1	2.7	3.2	2.4	2.1	4.1	3.2	3.4	15	15	15
sum	2.2	2.3	2.3	2.5	2.5	2.5	3.3	3.4	3.4	15	15	15

BPIFB1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1490 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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BPIFB1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1490 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments