LRIG1, CPTAC-1495 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LRIG1	LIG1

Sequence Length (AA)	Molecular Weight (Da)
1093	119113

Protein Name
Leucine-rich repeats and immunoglobulin-like domains protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARPVRGGLG	APRRSPCLLL	LWLLLLRLEP	VTAAAGPRAP	CAAACTCAGD
60	70	80	90	100
SLDCGGRGLA	ALPGDLPSWT	RSLNLSYNKL	SEIDPAGFED	LPNLQEVYLN
110	120	130	140	150
NNELTAVPSL	GAASSHVVSL	FLQHNKIRSV	EGSQLKAYLS	LEVLDLSLNN
160	170	180	190	200
ITEVRNTCFP	HGPPIKELNL	AGNRIGTLEL	GAFDGLSRSL	LTLRLSKNRI
210	220	230	240	250
TQLPVRAFKL	PRLTQLDLNR	NRIRLIEGLT	FQGLNSLEVL	KLQRNNISKL
260	270	280	290	300
TDGAFWGLSK	MHVLHLEYNS	LVEVNSGSLY	GLTALHQLHL	SNNSIARIHR
310	320	330	340	350
KGWSFCQKLH	ELVLSFNNLT	RLDEESLAEL	SSLSVLRLSH	NSISHIAEGA
360	370	380	390	400
FKGLRSLRVL	DLDHNEISGT	IEDTSGAFSG	LDSLSKLTLF	GNKIKSVAKR
410	420	430	440	450
AFSGLEGLEH	LNLGGNAIRS	VQFDAFVKMK	NLKELHISSD	SFLCDCQLKW
460	470	480	490	500
LPPWLIGRML	QAFVTATCAH	PESLKGQSIF	SVPPESFVCD	DFLKPQIITQ
510	520	530	540	550
PETTMAMVGK	DIRFTCSAAS	SSSSPMTFAW	KKDNEVLTNA	DMENFVHVHA
560	570	580	590	600
QDGEVMEYTT	ILHLRQVTFG	HEGRYQCVIT	NHFGSTYSHK	ARLTVNVLPS
610	620	630	640	650
FTKTPHDITI	RTTTMARLEC	AATGHPNPQI	AWQKDGGTDF	PAARERRMHV
660	670	680	690	700
MPDDDVFFIT	DVKIDDAGVY	SCTAQNSAGS	ISANATLTVL	ETPSLVVPLE
710	720	730	740	750
DRVVSVGETV	ALQCKATGNP	PPRITWFKGD	RPLSLTERHH	LTPDNQLLVV
760	770	780	790	800
QNVVAEDAGR	YTCEMSNTLG	TERAHSQLSV	LPAAGCRKDG	TTVGIFTIAV
810	820	830	840	850
VSSIVLTSLV	WVCIIYQTRK	KSEEYSVTNT	DETVVPPDVP	SYLSSQGTLS
860	870	880	890	900
DRQETVVRTE	GGPQANGHIE	SNGVCPRDAS	HFPEPDTHSV	ACRQPKLCAG
910	920	930	940	950
SAYHKEPWKA	MEKAEGTPGP	HKMEHGGRVV	CSDCNTEVDC	YSRGQAFHPQ
960	970	980	990	1000
PVSRDSAQPS	APNGPEPGGS	DQEHSPHHQC	SRTAAGSCPE	CQGSLYPSNH
1010	1020	1030	1040	1050
DRMLTAVKKK	PMASLDGKGD	SSWTLARLYH	PDSTELQPAS	SLTSGSPERA
1060	1070	1080	1090	1093
EAQYLLVSNG	HLPKACDASP	ESTPLTGQLP	GKQRVPLLLA	PKS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1495 Collapse assay details

Data source: Panorama

Official Gene Symbol: LRIG1
Peptide Modified Sequence: LHELVLSFNN[+1.0]LTR
Modification Type: Deamidated (N)
Protein - Site of Modification: 318
Peptide - Site of Modification: 10
Peptide Start: 309
Peptide End: 321

CPTAC ID: CPTAC-1495
Peptide Molecular Mass: 1,555.8358
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	24.8	13.1	9.9	51.5	17.1	9.2	57.2	21.5	13.5	15	15	15
b5 (1+)	8.1	6.7	3.7	12.1	5.8	3.9	14.6	8.9	5.4	15	15	15
y5 (1+)	6.2	5.3	3.2	6.9	4.8	5.2	9.3	7.2	6.1	15	15	15
sum	6.5	4.2	2.4	11.9	4.8	3.2	13.6	6.4	4	15	15	15

LRIG1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1495 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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LRIG1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1495 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments