ITGA9, CPTAC-1492 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ITGA9	N/A

Sequence Length (AA)	Molecular Weight (Da)
1035	114489

Protein Name
Integrin alpha-9

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGGPAAPRGA	GRLRALLLAL	VVAGIPAGAY	NLDPQRPVHF	QGPADSFFGY
60	70	80	90	100
AVLEHFHDNT	RWVLVGAPKA	DSKYSPSVKS	PGAVFKCRVH	TNPDRRCTEL
110	120	130	140	150
DMARGKNRGT	SCGKTCREDR	DDEWMGVSLA	RQPKADGRVL	ACAHRWKNIY
160	170	180	190	200
YEADHILPHG	FCYIIPSNLQ	AKGRTLIPCY	EEYKKKYGEE	HGSCQAGIAG
210	220	230	240	250
FFTEELVVMG	APGSFYWAGT	IKVLNLTDNT	YLKLNDEVIM	NRRYTYLGYA
260	270	280	290	300
VTAGHFSHPS	TIDVVGGAPQ	DKGIGKVYIF	RADRRSGTLI	KIFQASGKKM
310	320	330	340	350
GSYFGSSLCA	VDLNGDGLSD	LLVGAPMFSE	IRDEGQVTVY	INRGNGALEE
360	370	380	390	400
QLALTGDGAY	NAHFGESIAS	LDDLDNDGFP	DVAIGAPKED	DFAGAVYIYH
410	420	430	440	450
GDAGGIVPQY	SMKLSGQKIN	PVLRMFGQSI	SGGIDMDGNG	YPDVTVGAFM
460	470	480	490	500
SDSVVLLRAR	PVITVDVSIF	LPGSINITAP	QCHDGQQPVN	CLNVTTCFSF
510	520	530	540	550
HGKHVPGEIG	LNYVLMADVA	KKEKGQMPRV	YFVLLGETMG	QVTEKLQLTY
560	570	580	590	600
MEETCRHYVA	HVKRRVQDVI	SPIVFEAAYS	LSEHVTGEEE	RELPPLTPVL
610	620	630	640	650
RWKKGQKIAQ	KNQTVFERNC	RSEDCAADLQ	LQGKLLLSSM	DEKTLYLALG
660	670	680	690	700
AVKNISLNIS	ISNLGDDAYD	ANVSFNVSRE	LFFINMWQKE	EMGISCELLE
710	720	730	740	750
SDFLKCSVGF	PFMRSKSKYE	FSVIFDTSHL	SGEEEVLSFI	VTAQSGNTER
760	770	780	790	800
SESLHDNTLV	LMVPLMHEVD	TSITGIMSPT	SFVYGESVDA	ANFIQLDDLE
810	820	830	840	850
CHFQPINITL	QVYNTGPSTL	PGSSVSISFP	NRLSSGGAEM	FHVQEMVVGQ
860	870	880	890	900
EKGNCSFQKN	PTPCIIPQEQ	ENIFHTIFAF	FTKSGRKVLD	CEKPGISCLT
910	920	930	940	950
AHCNFSALAK	EESRTIDIYM	LLNTEILKKD	SSSVIQFMSR	AKVKVDPALR
960	970	980	990	1000
VVEIAHGNPE	EVTVVFEALH	NLEPRGYVVG	WIIAISLLVG	ILIFLLLAVL
1010	1020	1030	1035
LWKMGFFRRR	YKEIIEAEKN	RKENEDSWDW	VQKNQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1492 Collapse assay details

Data source: Panorama

Official Gene Symbol: ITGA9
Peptide Modified Sequence: N[+1.0]QTVFER
Modification Type: Deamidated (N)
Protein - Site of Modification: 612
Peptide - Site of Modification: 1
Peptide Start: 612
Peptide End: 618

CPTAC ID: CPTAC-1492
Peptide Molecular Mass: 893.4243
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	7.3	12.8	19.4	13	12	26.5	14.9	17.5	32.8	15	15	15
y5 (1+)	6.6	10	7.1	6.6	10.1	7.5	9.3	14.2	10.3	15	15	15
y3 (1+)	8.4	15.5	15.4	10.6	13.1	16.3	13.5	20.3	22.4	15	15	15
sum	4.5	9	4.4	5.5	9	5.2	7.1	12.7	6.8	15	15	15

ITGA9

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1492 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

ITGA9

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1492 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments